FastQCFastQC Report
Thu 26 May 2016
SRR805228_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805228_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17791075
Sequences flagged as poor quality0
Sequence length53
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1150910.6469030117629204No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT653000.039.9643941
TCAACGC681400.038.87811712
CAACGCA681950.038.82953313
ATCAACG687750.038.55332611
GTGGTAT688000.038.3207136
GGTATCA689100.038.3141068
GTATCAA690450.038.235799
AACGCAG692600.038.2256714
ACGCAGA695250.038.0326515
AGTGGTA693500.038.0303545
CGCAGAG701050.037.74481216
TGGTATC699550.037.704817
TATCAAC700900.037.67241710
AGAGTAC705950.036.983519
CAGTGGT724350.036.4398424
GCAGTGG733250.036.2315033
CAGAGTA723950.036.19055618
AGCAGTG741300.035.920482
GCAGAGT745000.035.39825417
GTACATG508300.034.81772222
Produced by FastQC (version 0.11.5)