##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR805228_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 17791075 Sequences flagged as poor quality 0 Sequence length 53 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.174733005172538 35.0 28.0 35.0 19.0 37.0 2 34.58442499961357 35.0 33.0 37.0 32.0 37.0 3 37.52043690445912 39.0 37.0 39.0 35.0 39.0 4 37.99518561975597 39.0 38.0 39.0 35.0 39.0 5 38.01066495419754 39.0 39.0 39.0 35.0 39.0 6 38.06530875734041 39.0 39.0 39.0 35.0 39.0 7 38.05182278192858 39.0 39.0 39.0 35.0 39.0 8 39.0303651128445 40.0 40.0 40.0 37.0 40.0 9 39.093859814541844 40.0 40.0 40.0 37.0 40.0 10 38.96284715791485 40.0 40.0 40.0 37.0 40.0 11 39.078600702880514 40.0 40.0 40.0 37.0 40.0 12 39.11757659388204 40.0 40.0 40.0 37.0 40.0 13 39.13877059143419 40.0 40.0 40.0 37.0 40.0 14 39.10437800976051 40.0 40.0 40.0 37.0 40.0 15 39.07587472932355 40.0 40.0 40.0 37.0 40.0 16 39.010323771891244 40.0 40.0 40.0 37.0 40.0 17 38.956281955980735 40.0 40.0 40.0 37.0 40.0 18 38.93967194225194 40.0 40.0 40.0 37.0 40.0 19 38.910133592264664 40.0 40.0 40.0 37.0 40.0 20 38.88234488360035 40.0 40.0 40.0 37.0 40.0 21 38.84237647247286 40.0 39.0 40.0 37.0 40.0 22 38.876161446118346 40.0 40.0 40.0 37.0 40.0 23 38.72368578065125 40.0 39.0 40.0 36.0 40.0 24 38.76087268475907 40.0 39.0 40.0 36.0 40.0 25 38.800211566754676 40.0 39.0 40.0 36.0 40.0 26 38.82358069987339 40.0 40.0 40.0 37.0 40.0 27 38.834243012296895 40.0 40.0 40.0 37.0 40.0 28 38.81911402205881 40.0 40.0 40.0 36.0 40.0 29 38.786030411315785 40.0 40.0 40.0 36.0 40.0 30 38.68454615586748 40.0 39.0 40.0 36.0 40.0 31 38.69565436602341 40.0 39.0 40.0 36.0 40.0 32 38.67867624637634 40.0 39.0 40.0 36.0 40.0 33 38.57626146817997 40.0 39.0 40.0 35.0 40.0 34 38.578017854457926 40.0 39.0 40.0 35.0 40.0 35 38.54060370157509 40.0 39.0 40.0 35.0 40.0 36 38.468607995863096 40.0 39.0 40.0 35.0 40.0 37 38.41021506570008 40.0 39.0 40.0 35.0 40.0 38 38.250900746582204 40.0 38.0 40.0 35.0 40.0 39 38.257282148493 40.0 38.0 40.0 35.0 40.0 40 38.249677605203736 40.0 38.0 40.0 35.0 40.0 41 38.21055613559046 40.0 38.0 40.0 35.0 40.0 42 38.18299827301048 40.0 38.0 40.0 35.0 40.0 43 38.06247908010056 40.0 38.0 40.0 34.0 40.0 44 37.85994842919835 40.0 38.0 40.0 34.0 40.0 45 37.78191042418741 40.0 38.0 40.0 34.0 40.0 46 37.6967467676911 40.0 37.0 40.0 34.0 40.0 47 37.78320410655343 40.0 37.0 40.0 34.0 40.0 48 37.75845388769369 40.0 37.0 40.0 34.0 40.0 49 37.67203257813257 40.0 37.0 40.0 34.0 40.0 50 37.57027992968384 40.0 37.0 40.0 34.0 40.0 51 37.51151248589532 40.0 37.0 40.0 33.0 40.0 52 37.41661838871457 40.0 37.0 40.0 33.0 40.0 53 36.97207088385609 39.0 36.0 40.0 33.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 2.0 10 2.0 11 5.0 12 20.0 13 60.0 14 201.0 15 483.0 16 983.0 17 1729.0 18 2941.0 19 4147.0 20 6075.0 21 8746.0 22 12677.0 23 18082.0 24 25582.0 25 35128.0 26 47542.0 27 62787.0 28 82569.0 29 105524.0 30 133779.0 31 168423.0 32 207966.0 33 259952.0 34 340790.0 35 472830.0 36 722798.0 37 1318382.0 38 3216318.0 39 1.0534551E7 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 8.37738585217588 45.32936317788554 32.99838823679851 13.294862733140073 2 32.648926498258255 43.740240541957135 13.725078445231667 9.885754514552943 3 35.59650555123847 26.31459875246437 21.860415966994687 16.22847972930247 4 23.75050411512514 28.44263205005881 20.258494778983284 27.548369055832772 5 19.875724204411483 33.1718965829777 25.411415555271393 21.540963657339425 6 25.609441812819068 25.88797472890199 23.71272674641639 24.789856711862548 7 23.184995847637087 25.771989607148527 28.304427922427394 22.73858662278699 8 23.239444496749073 27.34430606357401 26.982422366270733 22.43382707340619 9 25.187921471861596 25.903257672737595 26.88740281292727 22.02141804247354 10 22.371784729140877 27.62245114474533 28.496108301493866 21.509655824619927 11 21.73933840422796 30.2349970420562 26.507791125606516 21.51787342810932 12 21.803786449104397 26.609881640092013 29.318790460947415 22.26754144985618 13 21.82398758928283 27.33932041768134 26.31328910703822 24.523402885997612 14 22.10068250513249 30.269750422613583 25.833329351936296 21.79623772031763 15 22.051657923987168 29.794039989151866 25.770438267502104 22.383863819358865 16 22.54113930720881 26.645877216525705 26.015437515720663 24.797545960544824 17 25.38701005981932 26.513872826684164 25.945745268343817 22.153371845152698 18 22.379788742389092 26.438177569371156 25.872185913442554 25.309847774797195 19 22.634410793052133 29.71238668826926 25.65743216753344 21.995770351145165 20 25.646494098866988 26.949119151035 25.62487651814182 21.77951023195619 21 22.89549675890861 29.289506114723252 25.631790097000884 22.183207029367253 22 25.30228218362297 26.578483874639392 26.178907120564666 21.940326821172977 23 22.45491629932424 26.638171105455967 29.117284930786923 21.789627664432867 24 22.68440215107856 28.984561079080386 25.68320351636987 22.64783325347119 25 22.126886655247084 26.80840252767188 25.909232578694652 25.15547823838638 26 22.46987323700226 28.479768648044036 27.026534371869044 22.023823743084662 27 22.81964411931263 26.00877687267352 28.82792636195396 22.34365264605989 28 24.690211243558917 26.39331799792874 26.776408957862298 22.140061800650045 29 24.917257669927196 26.406695491981232 26.62043749464268 22.055609343448893 30 25.003458194628486 25.968689356882596 26.580704088988437 22.44714835950048 31 23.827969923121564 26.777949055917084 26.915636070333015 22.478444950628333 32 23.4941171345745 27.01414051708511 27.152310919941602 22.33943142839879 33 23.67330248453227 26.552071755079442 26.931110121226514 22.843515639161772 34 23.119198811763763 27.06084370955662 27.046977206267748 22.772980272411868 35 23.01367399103202 27.06854982062635 27.24276076628309 22.675015422058532 36 23.36015108699165 26.6762744803223 27.124274390389562 22.83930004229649 37 23.245897170350865 27.093185768707063 27.052609243679765 22.608307817262308 38 23.187440893818952 26.962625923391364 27.391689372339783 22.4582438104499 39 23.30884446274326 26.595559852341694 27.285804820675537 22.809790864239513 40 23.032689143292355 27.10927248634498 27.131064311740577 22.72697405862209 41 23.177638225908215 27.039495927030828 27.191510349992903 22.59135549706805 42 23.33426732223882 26.759479120851324 27.053907647514276 22.852345909395584 43 23.031531259353354 27.145020747762572 27.22259335087959 22.60085464200449 44 23.134150128645963 26.905001524640866 27.307495471746368 22.653352874966803 45 23.39879405825674 26.56476351204185 27.09058334024223 22.94585908945918 46 23.254300260102326 26.93343150990033 26.934802984080502 22.87746524591684 47 23.23568980514106 26.910093965654124 27.08259056858565 22.771625660619158 48 23.296192051351593 26.542803062771643 27.11077885962484 23.050226026251927 49 23.030480170534943 26.991022183875906 27.033920097577013 22.944577548012134 50 23.34487938474769 26.94271706459559 27.0507150354883 22.66168851516842 51 23.41958538199631 26.550582244187044 27.081309027138605 22.94852334667804 52 23.293814454719573 26.90493407509102 26.98356338782226 22.81768808236714 53 23.477457095762905 26.902809414270916 26.852986680119106 22.76674680984707 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1030.0 1 851.0 2 672.0 3 716.0 4 760.0 5 830.5 6 901.0 7 1117.0 8 1609.5 9 1886.0 10 2805.5 11 3725.0 12 5524.5 13 7324.0 14 10129.5 15 12935.0 16 19067.5 17 25200.0 18 35255.5 19 45311.0 20 61703.5 21 78096.0 22 170327.5 23 262559.0 24 230305.0 25 224820.0 26 251589.0 27 290413.5 28 329238.0 29 372039.0 30 414840.0 31 452804.5 32 490769.0 33 544690.5 34 598612.0 35 663585.5 36 728559.0 37 793152.5 38 857746.0 39 918718.5 40 979691.0 41 1040314.5 42 1144045.0 43 1187152.0 44 1227782.5 45 1268413.0 46 1284831.0 47 1301249.0 48 1302963.0 49 1304677.0 50 1280404.0 51 1256131.0 52 1207918.5 53 1159706.0 54 1090480.5 55 1021255.0 56 945950.5 57 870646.0 58 767473.0 59 577168.5 60 490037.0 61 419849.0 62 349661.0 63 292447.5 64 235234.0 65 188808.5 66 142383.0 67 110840.0 68 79297.0 69 58432.0 70 37567.0 71 27187.0 72 16807.0 73 12365.0 74 7923.0 75 5660.0 76 2488.0 77 1579.0 78 1215.5 79 852.0 80 665.5 81 479.0 82 373.5 83 268.0 84 194.5 85 121.0 86 91.0 87 61.0 88 54.5 89 48.0 90 39.5 91 31.0 92 21.0 93 9.0 94 7.0 95 10.0 96 13.0 97 9.0 98 5.0 99 2.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 52 0.0 53 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 53 1.7791075E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.58116011547705 #Duplication Level Percentage of deduplicated Percentage of total 1 72.5759737961217 38.161288987105415 2 14.949378964249089 15.721113778922513 3 5.164935511980541 8.147349034246867 4 2.3904177352465963 5.027637507195092 5 1.2859907987663284 3.380944404848127 6 0.8340529163691429 2.631328196423189 7 0.5570234930791069 2.0502259034372394 8 0.3889757722071033 1.6362237887570419 9 0.2757224205400703 1.30480242676599 >10 1.4444188000800944 13.501126321431212 >50 0.09084562028198584 3.302779283978785 >100 0.04069194387898456 3.6034620352362134 >500 0.001240658473939881 0.4306168506282574 >1k 2.6743236030266293E-4 0.24922535074751134 >5k 4.275757674149646E-5 0.14679902595333086 >10k+ 2.13787883707476E-5 0.7050771043232702 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 115091 0.6469030117629204 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 2.023486495335442E-4 0.0 0.0 0.0 0.0 2 5.002508280134843E-4 0.0 0.0 0.0 0.0 3 5.002508280134843E-4 0.0 0.0 5.620795820376228E-6 0.0 4 5.17113215474613E-4 0.0 0.0 5.620795820376228E-6 5.620795820376228E-6 5 5.339756029357417E-4 0.0 0.0 5.620795820376228E-6 1.1241591640752455E-5 6 5.395963987561179E-4 5.620795820376228E-6 0.0 5.620795820376228E-6 1.1241591640752455E-5 7 5.67700377857999E-4 5.620795820376228E-6 0.0 5.620795820376228E-6 1.1241591640752455E-5 8 5.733211736783753E-4 5.620795820376228E-6 0.0 5.620795820376228E-6 1.6862387461128684E-5 9 6.126667444210089E-4 5.620795820376228E-6 0.0 5.620795820376228E-6 2.248318328150491E-5 10 6.688747026247712E-4 5.620795820376228E-6 0.0 1.1241591640752455E-5 3.372477492225737E-5 11 7.194618650081571E-4 5.620795820376228E-6 0.0 1.1241591640752455E-5 4.496636656300982E-5 12 8.543609646971867E-4 5.620795820376228E-6 0.0 1.6862387461128684E-5 5.058716238338605E-5 13 0.0012253334888420178 1.1241591640752455E-5 0.0 1.6862387461128684E-5 5.058716238338605E-5 14 0.0014895108923997005 1.1241591640752455E-5 0.0 3.372477492225737E-5 5.058716238338605E-5 15 0.0021583855950244716 1.1241591640752455E-5 0.0 3.372477492225737E-5 5.058716238338605E-5 16 0.0022651807156116198 1.1241591640752455E-5 0.0 3.372477492225737E-5 5.620795820376228E-5 17 0.0023382510612765107 1.1241591640752455E-5 0.0 3.372477492225737E-5 5.620795820376228E-5 18 0.0029003306433141337 1.1241591640752455E-5 0.0 3.372477492225737E-5 6.18287540241385E-5 19 0.003012746559721658 2.248318328150491E-5 0.0 3.372477492225737E-5 6.744954984451474E-5 20 0.0031364040677699353 2.248318328150491E-5 0.0 3.372477492225737E-5 7.307034566489096E-5 21 0.0033218903298423506 2.248318328150491E-5 0.0 3.372477492225737E-5 7.307034566489096E-5 22 0.00350175579609439 2.248318328150491E-5 0.0 4.496636656300982E-5 7.307034566489096E-5 23 0.00379965797457433 2.248318328150491E-5 0.0 5.058716238338605E-5 8.431193730564342E-5 24 0.004091939357233894 2.248318328150491E-5 0.0 6.18287540241385E-5 8.431193730564342E-5 25 0.004316771190048943 2.248318328150491E-5 0.0 6.744954984451474E-5 8.431193730564342E-5 26 0.0046483981434511405 2.248318328150491E-5 0.0 6.744954984451474E-5 8.993273312601964E-5 27 0.005452171945764941 2.248318328150491E-5 0.0 6.744954984451474E-5 1.011743247667721E-4 28 0.005755694920065257 2.248318328150491E-5 0.0 6.744954984451474E-5 1.2927830386865325E-4 29 0.007267688995746463 2.248318328150491E-5 0.0 6.744954984451474E-5 1.2927830386865325E-4 30 0.00764428231571167 2.248318328150491E-5 0.0 7.307034566489096E-5 1.2927830386865325E-4 31 0.0087009919299424 2.248318328150491E-5 0.0 7.307034566489096E-5 1.2927830386865325E-4 32 0.009173138778854005 2.248318328150491E-5 0.0 7.307034566489096E-5 1.4614069132978192E-4 33 0.009454178569872816 2.248318328150491E-5 0.0 7.307034566489096E-5 1.4614069132978192E-4 34 0.00974645995253238 2.248318328150491E-5 0.0 7.307034566489096E-5 1.4614069132978192E-4 35 0.010100570089216082 2.248318328150491E-5 0.0 7.86911414852672E-5 1.573822829705344E-4 36 0.01062330410051107 2.248318328150491E-5 0.0 7.86911414852672E-5 1.6300307879091062E-4 37 0.011320282782237723 2.248318328150491E-5 0.0 7.86911414852672E-5 1.7424467043166306E-4 38 0.011944191118299485 2.810397910188114E-5 0.0 7.86911414852672E-5 1.7424467043166306E-4 39 0.012213989317677544 3.372477492225737E-5 0.0 7.86911414852672E-5 1.7424467043166306E-4 40 0.01259058263764275 3.93455707426336E-5 0.0 8.431193730564342E-5 1.7424467043166306E-4 41 0.01300652152835059 3.93455707426336E-5 0.0 8.993273312601964E-5 1.9110705789279176E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AAGCAGT 65300 0.0 39.964394 1 TCAACGC 68140 0.0 38.878117 12 CAACGCA 68195 0.0 38.829533 13 ATCAACG 68775 0.0 38.553326 11 GTGGTAT 68800 0.0 38.320713 6 GGTATCA 68910 0.0 38.314106 8 GTATCAA 69045 0.0 38.23579 9 AACGCAG 69260 0.0 38.22567 14 ACGCAGA 69525 0.0 38.03265 15 AGTGGTA 69350 0.0 38.030354 5 CGCAGAG 70105 0.0 37.744812 16 TGGTATC 69955 0.0 37.70481 7 TATCAAC 70090 0.0 37.672417 10 AGAGTAC 70595 0.0 36.9835 19 CAGTGGT 72435 0.0 36.439842 4 GCAGTGG 73325 0.0 36.231503 3 CAGAGTA 72395 0.0 36.190556 18 AGCAGTG 74130 0.0 35.92048 2 GCAGAGT 74500 0.0 35.398254 17 GTACATG 50830 0.0 34.817722 22 >>END_MODULE