FastQCFastQC Report
Thu 26 May 2016
SRR805227_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805227_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14147208
Sequences flagged as poor quality0
Sequence length53
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT899830.6360477629225498No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT571000.041.1270561
TCAACGC569150.041.05842212
CAACGCA570000.040.9930713
ATCAACG574000.040.71968511
GGTATCA576700.040.402728
GTGGTAT577200.040.3432966
AACGCAG580200.040.28050614
GTATCAA581000.040.144159
AGTGGTA583000.039.9782145
ACGCAGA585500.039.90384315
TATCAAC585650.039.80534410
TGGTATC585650.039.769237
CGCAGAG587450.039.7313816
AGAGTAC584400.039.399919
CAGAGTA600300.038.4385318
CAGTGGT611300.038.177414
GCAGTGG617750.037.988023
GTACATG446200.037.49888222
AGCAGTG628100.037.4780272
GCAGAGT622600.037.20896517
Produced by FastQC (version 0.11.5)