##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805227_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	14147208
Sequences flagged as poor quality	0
Sequence length	53
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.739680861410957	35.0	28.0	37.0	19.0	37.0
2	34.88499568254033	35.0	33.0	37.0	33.0	37.0
3	37.549920662790846	39.0	37.0	39.0	35.0	39.0
4	38.069451159550354	39.0	39.0	39.0	37.0	39.0
5	38.09841913683604	39.0	39.0	39.0	37.0	39.0
6	38.13894423549862	39.0	39.0	39.0	37.0	39.0
7	38.109626789964494	39.0	39.0	39.0	37.0	39.0
8	39.03859100679088	40.0	40.0	40.0	37.0	40.0
9	39.10712467081844	40.0	40.0	40.0	37.0	40.0
10	38.966361207101784	40.0	40.0	40.0	37.0	40.0
11	39.09496679486157	40.0	40.0	40.0	37.0	40.0
12	39.14093360329473	40.0	40.0	40.0	38.0	40.0
13	39.18326103638258	40.0	40.0	40.0	38.0	40.0
14	39.15433165328452	40.0	40.0	40.0	38.0	40.0
15	39.130208024085036	40.0	40.0	40.0	37.0	40.0
16	39.07867425148481	40.0	40.0	40.0	37.0	40.0
17	39.01934897684405	40.0	40.0	40.0	37.0	40.0
18	38.9920526368171	40.0	40.0	40.0	37.0	40.0
19	38.9687329118226	40.0	40.0	40.0	37.0	40.0
20	38.944638970459756	40.0	40.0	40.0	37.0	40.0
21	38.8963915000048	40.0	40.0	40.0	37.0	40.0
22	38.914512248635916	40.0	40.0	40.0	37.0	40.0
23	38.746383173273486	40.0	39.0	40.0	36.0	40.0
24	38.802816428513665	40.0	39.0	40.0	37.0	40.0
25	38.84110942597295	40.0	40.0	40.0	37.0	40.0
26	38.86628068237917	40.0	40.0	40.0	37.0	40.0
27	38.87559036383716	40.0	40.0	40.0	37.0	40.0
28	38.84951087168578	40.0	40.0	40.0	37.0	40.0
29	38.80805958320539	40.0	40.0	40.0	37.0	40.0
30	38.70085828949429	40.0	39.0	40.0	36.0	40.0
31	38.714229479060464	40.0	39.0	40.0	36.0	40.0
32	38.68858463097454	40.0	39.0	40.0	36.0	40.0
33	38.63405528497213	40.0	39.0	40.0	36.0	40.0
34	38.623850303183495	40.0	39.0	40.0	36.0	40.0
35	38.594675076523934	40.0	39.0	40.0	35.0	40.0
36	38.52185392340312	40.0	39.0	40.0	35.0	40.0
37	38.4734100891144	40.0	39.0	40.0	35.0	40.0
38	38.32085574764999	40.0	39.0	40.0	35.0	40.0
39	38.314031998398555	40.0	38.0	40.0	35.0	40.0
40	38.30294853938671	40.0	38.0	40.0	35.0	40.0
41	38.26624659791529	40.0	38.0	40.0	35.0	40.0
42	38.218872020542854	40.0	38.0	40.0	35.0	40.0
43	38.10425187782635	40.0	38.0	40.0	35.0	40.0
44	37.98302279856209	40.0	38.0	40.0	34.0	40.0
45	37.89608719967926	40.0	38.0	40.0	34.0	40.0
46	37.80088735530007	40.0	38.0	40.0	34.0	40.0
47	37.90273282191087	40.0	38.0	40.0	34.0	40.0
48	37.896229842665775	40.0	38.0	40.0	34.0	40.0
49	37.81500484053108	40.0	38.0	40.0	34.0	40.0
50	37.70953533729058	40.0	37.0	40.0	34.0	40.0
51	37.62460409149282	40.0	37.0	40.0	34.0	40.0
52	37.54303251920803	40.0	37.0	40.0	34.0	40.0
53	37.15917593068541	39.0	36.0	40.0	33.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	6.0
11	19.0
12	58.0
13	98.0
14	247.0
15	492.0
16	1007.0
17	1819.0
18	2628.0
19	3933.0
20	5482.0
21	7602.0
22	10631.0
23	15143.0
24	20825.0
25	28775.0
26	38532.0
27	50866.0
28	65379.0
29	83384.0
30	105345.0
31	130878.0
32	160739.0
33	196987.0
34	255491.0
35	350410.0
36	523675.0
37	930696.0
38	2245112.0
39	8910948.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	12.331366019358732	35.90294989654495	28.80567671020317	22.960007373893138
2	29.8396757862046	40.815622418218496	13.899109986931697	15.445591808645212
3	30.667938154298714	27.91564950483516	20.34643867539093	21.069973665475196
4	22.666196750623868	26.594887132499924	21.40220883159419	29.336707285282017
5	21.26249221754568	30.31542336834236	25.417460462870128	23.00462395124183
6	26.195182823352848	25.403662687365593	24.134557150781976	24.266597338499583
7	23.53969772692958	25.140006423882365	28.138152771910896	23.182143077277157
8	25.18851069412424	26.90613582552826	25.576205566497645	22.329147913849855
9	25.93016233309074	26.019473241645986	25.721753719885932	22.328610705377343
10	22.384706579559726	26.76561339877098	28.623852847855208	22.225827173814082
11	22.1202162292376	30.07497309716518	25.601115075144154	22.203695598453066
12	21.94756732211755	26.201551571165137	29.068081843428047	22.782799263289267
13	22.053687201036418	26.523813037879982	25.729663407790426	25.692836353293174
14	22.51461913898488	30.03656269138052	25.25461561037344	22.194202559261164
15	22.32927514743545	29.668638504502088	25.287993220994558	22.7140931270679
16	22.636798723818863	26.01976305147984	25.56315705544161	25.780281169259688
17	26.055741882073125	26.001476757816803	25.43892052764051	22.503860832469556
18	22.399663594399684	26.04732325982625	25.51265945902541	26.040353686748652
19	22.76023650744373	29.469411915057727	25.35716588036311	22.413185697135436
20	26.235480527323833	26.240287129446322	25.301953572747358	22.222278770482486
21	22.757797863719823	29.34193799935648	25.400432367997983	22.499831768925713
22	25.8572716256098	25.848160287174686	25.81527747383088	22.479290613384634
23	22.572065102881076	26.151407401375593	29.032300931745684	22.244226563997646
24	22.60447432454517	29.12631948296795	25.444228995572836	22.82497719691405
25	22.36998282629336	26.087295811300713	25.63659910846013	25.906122253945796
26	22.702811749145134	28.40055790513577	26.553946192068427	22.342684153650673
27	22.815738624893335	25.786367175770653	28.84902095169591	22.5488732476401
28	25.166774956585076	25.848817660700256	26.43636115338094	22.548046229333732
29	25.430487768328565	25.989234059469545	26.212055410509265	22.368222761692625
30	25.298779801640016	25.750522647295494	26.244089999949104	22.706607551115386
31	24.128534761063808	26.278188600888598	26.63635114433887	22.956925493708724
32	23.728689081266072	26.726814223696998	26.763733169117188	22.780763525919742
33	23.692915238116242	26.363018059817882	26.75923758242616	23.18482911963972
34	23.27851544983293	26.59434285549488	26.881798868016926	23.245342826655264
35	23.2844106059655	26.72214192369265	26.89406984049432	23.099377629847528
36	23.347221586054296	26.55572746226676	26.87801720311174	23.21903374856721
37	23.43369801306378	26.655337222722675	26.87082143699308	23.040143327220466
38	23.528494102864677	26.637913290028674	26.9545694104448	22.879023196661844
39	23.44415944121271	26.39112961370187	27.04296847830328	23.121742466782138
40	23.283060516251687	26.620496425867206	26.95688788911565	23.139555168765455
41	23.445050076311876	26.73200252657627	26.847417525776113	22.97552987133574
42	23.43940231881796	26.574621649727636	26.873415588432714	23.11256044302169
43	23.29850525983643	26.617464025410527	27.01084199794051	23.07318871681253
44	23.43339406616486	26.60246459937537	26.919841710109864	23.044299624349907
45	23.4896313109979	26.322543642533564	26.909599406469457	23.27822563999907
46	23.493936047310537	26.483161907282344	26.759993915407193	23.262908129999925
47	23.50627063658073	26.561191437914815	26.823179527720242	23.109358397784213
48	23.437027291886853	26.411918167881606	26.848753478424857	23.30230106180668
49	23.30730558284009	26.534019998857726	26.841105326224085	23.3175690920781
50	23.582172538920755	26.586935033400227	26.739565856386644	23.09132657129237
51	23.48365133247493	26.41569276425426	26.844731483413547	23.25592441985726
52	23.402349071279648	26.470544576710825	26.83746503196956	23.28964132003997
53	23.717075482314247	26.530132305964543	26.579124304951197	23.173667906770014
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	260.0
1	206.5
2	153.0
3	158.5
4	164.0
5	209.5
6	255.0
7	304.0
8	489.0
9	625.0
10	1125.0
11	1625.0
12	2618.0
13	3611.0
14	4981.5
15	6352.0
16	9695.5
17	13039.0
18	19205.5
19	25372.0
20	36329.5
21	47287.0
22	113647.5
23	180008.0
24	154105.5
25	145218.5
26	162234.0
27	188696.0
28	215158.0
29	247872.0
30	280586.0
31	308618.0
32	336650.0
33	380748.5
34	424847.0
35	477103.5
36	529360.0
37	580616.5
38	631873.0
39	684545.0
40	737217.0
41	791849.5
42	885077.0
43	923672.0
44	965066.5
45	1006461.0
46	1022671.5
47	1038882.0
48	1052777.5
49	1066673.0
50	1054403.5
51	1042134.0
52	1007341.5
53	972549.0
54	924567.0
55	876585.0
56	819300.5
57	762016.0
58	680362.0
59	524381.0
60	450054.0
61	387677.5
62	325301.0
63	273653.0
64	222005.0
65	179038.5
66	136072.0
67	107849.5
68	79627.0
69	60033.5
70	40440.0
71	29431.5
72	18423.0
73	13438.5
74	8454.0
75	6218.0
76	2834.5
77	1687.0
78	1257.5
79	828.0
80	646.0
81	464.0
82	360.0
83	256.0
84	172.0
85	88.0
86	70.0
87	52.0
88	39.5
89	27.0
90	24.0
91	21.0
92	18.0
93	11.0
94	7.0
95	6.0
96	5.0
97	5.5
98	6.0
99	3.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	1.4147208E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.444025143112725
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.14343464868024	39.8222299506637
2	14.773447250834792	16.08651867149798
3	4.983739587429729	8.140045302142513
4	2.4275373360475445	5.286596150384696
5	1.3249091234211257	3.6066692813939607
6	0.8013059439514023	2.61757925758895
7	0.5128569737342111	1.9545398580964268
8	0.361855045682505	1.5760676164240408
9	0.27277966348378074	1.336610057151671
>10	1.276228306502538	12.22656753651731
>50	0.08678062219865809	3.2561156526957387
>100	0.03450978884417811	3.0097943081512697
>500	4.467594900700835E-4	0.15495890907736848
>1k	1.2996243241805908E-4	0.1974140183446104
>5k	2.599151121871675E-5	0.09162659124688043
>10k+	1.2995755609358376E-5	0.6366668386230148
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	89983	0.6360477629225498	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.1101543145474356E-4	7.068532533062354E-6	0.0	1.4137065066124708E-5	7.068532533062354E-6
2	0.001816612860997025	7.068532533062354E-6	0.0	2.1205597599187062E-5	7.068532533062354E-6
3	0.0018307499260631498	7.068532533062354E-6	0.0	2.8274130132249415E-5	1.4137065066124708E-5
4	0.0018307499260631498	7.068532533062354E-6	0.0	3.534266266531177E-5	1.4137065066124708E-5
5	0.0018448869911292744	2.1205597599187062E-5	0.0	4.947972773143648E-5	3.534266266531177E-5
6	0.0018590240561953992	2.1205597599187062E-5	0.0	5.654826026449883E-5	4.2411195198374125E-5
7	0.0018590240561953992	2.1205597599187062E-5	0.0	7.068532533062354E-5	4.2411195198374125E-5
8	0.0018590240561953992	2.1205597599187062E-5	7.068532533062354E-6	8.482239039674825E-5	4.2411195198374125E-5
9	0.0018660925887284616	2.1205597599187062E-5	7.068532533062354E-6	9.895945546287296E-5	4.947972773143648E-5
10	0.0019297093815260227	3.534266266531177E-5	7.068532533062354E-6	9.895945546287296E-5	5.654826026449883E-5
11	0.001993326174323584	4.2411195198374125E-5	7.068532533062354E-6	1.0602798799593531E-4	6.361679279756118E-5
12	0.0020993541623195193	4.2411195198374125E-5	7.068532533062354E-6	1.1309652052899766E-4	9.895945546287296E-5
13	0.0028132759481588167	4.2411195198374125E-5	7.068532533062354E-6	1.2016505306206002E-4	9.895945546287296E-5
14	0.0029970577940184383	4.2411195198374125E-5	7.068532533062354E-6	1.3430211812818474E-4	1.1309652052899766E-4
15	0.0034989236038658654	4.947972773143648E-5	7.068532533062354E-6	1.5550771572737178E-4	1.1309652052899766E-4
16	0.0036968425147916113	6.361679279756118E-5	7.068532533062354E-6	1.696447807934965E-4	1.2016505306206002E-4
17	0.003774596372655297	6.361679279756118E-5	7.068532533062354E-6	1.7671331332655884E-4	1.3430211812818474E-4
18	0.004318873377701099	6.361679279756118E-5	7.068532533062354E-6	1.9085037839268356E-4	1.3430211812818474E-4
19	0.004446106963296221	7.068532533062354E-5	7.068532533062354E-6	1.9085037839268356E-4	1.3430211812818474E-4
20	0.004587477613957468	7.068532533062354E-5	7.068532533062354E-6	1.979189109257459E-4	1.696447807934965E-4
21	0.004990383968342022	7.775385786368589E-5	7.068532533062354E-6	2.1912450852493298E-4	1.696447807934965E-4
22	0.00510348048887102	7.775385786368589E-5	7.068532533062354E-6	2.2619304105799532E-4	1.696447807934965E-4
23	0.005350879127528202	7.775385786368589E-5	7.068532533062354E-6	2.2619304105799532E-4	1.696447807934965E-4
24	0.00582447080724338	7.775385786368589E-5	7.068532533062354E-6	2.332615735910577E-4	1.7671331332655884E-4
25	0.006121349173631998	8.482239039674825E-5	7.068532533062354E-6	2.4033010612412004E-4	1.7671331332655884E-4
26	0.006467707267752054	8.482239039674825E-5	7.068532533062354E-6	2.4033010612412004E-4	1.8378184585962122E-4
27	0.0072947255741203495	9.189092292981061E-5	7.068532533062354E-6	2.4033010612412004E-4	1.9085037839268356E-4
28	0.007542124212777532	9.189092292981061E-5	7.068532533062354E-6	2.473986386571824E-4	1.9085037839268356E-4
29	0.008171223608220082	9.189092292981061E-5	7.068532533062354E-6	2.473986386571824E-4	1.9085037839268356E-4
30	0.008489307572207887	9.895945546287296E-5	7.068532533062354E-6	2.5446717119024473E-4	1.9085037839268356E-4
31	0.00933753147617537	1.1309652052899766E-4	7.068532533062354E-6	2.615357037233071E-4	1.9085037839268356E-4
32	0.009931288208952607	1.2723358559512237E-4	7.068532533062354E-6	2.756727687894318E-4	1.9085037839268356E-4
33	0.010214029510275101	1.2723358559512237E-4	7.068532533062354E-6	2.898098338555565E-4	1.979189109257459E-4
34	0.010588661734527407	1.4843918319430943E-4	7.068532533062354E-6	2.9687836638861887E-4	1.979189109257459E-4
35	0.010871403035849901	1.5550771572737178E-4	7.068532533062354E-6	2.9687836638861887E-4	1.979189109257459E-4
36	0.011295514987833643	1.6257624826043415E-4	7.068532533062354E-6	3.1101543145474356E-4	2.1912450852493298E-4
37	0.011917545850743129	1.696447807934965E-4	1.4137065066124708E-5	3.251524965208683E-4	2.2619304105799532E-4
38	0.012278041009929309	1.696447807934965E-4	1.4137065066124708E-5	3.3222102905393065E-4	2.332615735910577E-4
39	0.012610262038983239	1.696447807934965E-4	1.4137065066124708E-5	3.3222102905393065E-4	2.332615735910577E-4
40	0.01287886627523961	1.696447807934965E-4	1.4137065066124708E-5	3.3222102905393065E-4	2.4033010612412004E-4
41	0.013218155836826602	1.8378184585962122E-4	1.4137065066124708E-5	3.39289561586993E-4	2.615357037233071E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	57100	0.0	41.127056	1
TCAACGC	56915	0.0	41.058422	12
CAACGCA	57000	0.0	40.99307	13
ATCAACG	57400	0.0	40.719685	11
GGTATCA	57670	0.0	40.40272	8
GTGGTAT	57720	0.0	40.343296	6
AACGCAG	58020	0.0	40.280506	14
GTATCAA	58100	0.0	40.14415	9
AGTGGTA	58300	0.0	39.978214	5
ACGCAGA	58550	0.0	39.903843	15
TATCAAC	58565	0.0	39.805344	10
TGGTATC	58565	0.0	39.76923	7
CGCAGAG	58745	0.0	39.73138	16
AGAGTAC	58440	0.0	39.3999	19
CAGAGTA	60030	0.0	38.43853	18
CAGTGGT	61130	0.0	38.17741	4
GCAGTGG	61775	0.0	37.98802	3
GTACATG	44620	0.0	37.498882	22
AGCAGTG	62810	0.0	37.478027	2
GCAGAGT	62260	0.0	37.208965	17
>>END_MODULE