FastQCFastQC Report
Thu 26 May 2016
SRR805226_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805226_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14388610
Sequences flagged as poor quality0
Sequence length53
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGTA96850.041.15229810
CTCGTAT97400.040.84753811
TATGCCG98350.040.35739515
TCGTATG101450.039.2400212
ATGCCGT100850.039.21715516
CGTATGC103850.038.37843313
GAGCTCG104250.038.1860928
TGCCGTC103350.038.086617
GCCGTCT107100.036.7969218
AGCTCGT109050.036.5052729
CCGTCTT110000.035.95519
ATCGGAA114600.035.0244331
GTATGCC114300.034.9518814
CGGAAGA121850.032.612643
TCGGAAG123900.032.3006442
CGTCTTC126450.031.3519220
GCTTGAA132250.029.85255228
TGCTTGA141450.027.96076427
AGAGCTC144400.027.8289477
CTTGAAA151300.026.10938529
Produced by FastQC (version 0.11.5)