##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805226_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	14388610
Sequences flagged as poor quality	0
Sequence length	53
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.730211813371827	35.0	28.0	37.0	17.0	37.0
2	34.92519068902416	35.0	33.0	37.0	33.0	37.0
3	37.61379222871424	39.0	37.0	39.0	35.0	39.0
4	38.17044787509009	39.0	39.0	39.0	37.0	39.0
5	38.21854077635018	39.0	39.0	39.0	37.0	39.0
6	38.20522851060665	39.0	39.0	39.0	37.0	39.0
7	38.21982741904882	39.0	39.0	39.0	37.0	39.0
8	39.1451823351943	40.0	40.0	40.0	38.0	40.0
9	39.173832149179105	40.0	40.0	40.0	38.0	40.0
10	39.161898612861144	40.0	40.0	40.0	38.0	40.0
11	39.21552095720156	40.0	40.0	40.0	38.0	40.0
12	39.218196476240585	40.0	40.0	40.0	38.0	40.0
13	39.24604989641112	40.0	40.0	40.0	38.0	40.0
14	39.21092120781646	40.0	40.0	40.0	38.0	40.0
15	39.172042747701134	40.0	40.0	40.0	38.0	40.0
16	39.12652799679746	40.0	40.0	40.0	38.0	40.0
17	39.074452917967754	40.0	40.0	40.0	37.0	40.0
18	39.046037456015554	40.0	40.0	40.0	37.0	40.0
19	39.01967750880731	40.0	40.0	40.0	37.0	40.0
20	38.99184660644774	40.0	40.0	40.0	37.0	40.0
21	38.9695691244672	40.0	40.0	40.0	37.0	40.0
22	38.95697200772	40.0	40.0	40.0	37.0	40.0
23	38.937640328009444	40.0	40.0	40.0	37.0	40.0
24	38.923083049717796	40.0	40.0	40.0	37.0	40.0
25	38.9162798213309	40.0	40.0	40.0	37.0	40.0
26	38.93126577202384	40.0	40.0	40.0	37.0	40.0
27	38.93651096249047	40.0	40.0	40.0	37.0	40.0
28	38.90605937613154	40.0	40.0	40.0	37.0	40.0
29	38.867611395402335	40.0	40.0	40.0	37.0	40.0
30	38.754317825001856	40.0	40.0	40.0	36.0	40.0
31	38.78075171958931	40.0	40.0	40.0	37.0	40.0
32	38.75685184322877	40.0	39.0	40.0	36.0	40.0
33	38.680950696418904	40.0	39.0	40.0	36.0	40.0
34	38.68925671068991	40.0	39.0	40.0	36.0	40.0
35	38.66451700337976	40.0	39.0	40.0	36.0	40.0
36	38.601527597175824	40.0	39.0	40.0	36.0	40.0
37	38.56281815964155	40.0	39.0	40.0	35.0	40.0
38	38.432967395738714	40.0	39.0	40.0	35.0	40.0
39	38.353850302426714	40.0	39.0	40.0	35.0	40.0
40	38.32811300049136	40.0	39.0	40.0	35.0	40.0
41	38.28277227612674	40.0	39.0	40.0	35.0	40.0
42	38.24067293505071	40.0	38.0	40.0	35.0	40.0
43	38.13506739010926	40.0	38.0	40.0	35.0	40.0
44	38.01318563780657	40.0	38.0	40.0	35.0	40.0
45	37.92800423390446	40.0	38.0	40.0	34.0	40.0
46	37.83787454104323	40.0	38.0	40.0	34.0	40.0
47	37.9540417038199	40.0	38.0	40.0	34.0	40.0
48	37.96377690409289	40.0	38.0	40.0	34.0	40.0
49	37.90923376198257	40.0	38.0	40.0	34.0	40.0
50	37.8203366412739	40.0	38.0	40.0	34.0	40.0
51	37.76582567739344	40.0	38.0	40.0	34.0	40.0
52	37.704176636937135	40.0	37.0	40.0	34.0	40.0
53	37.3274606789676	40.0	37.0	40.0	33.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	4.0
12	16.0
13	37.0
14	114.0
15	323.0
16	691.0
17	1306.0
18	2138.0
19	3042.0
20	4717.0
21	6780.0
22	9568.0
23	14072.0
24	19608.0
25	27136.0
26	36903.0
27	48920.0
28	64071.0
29	84097.0
30	113365.0
31	143263.0
32	156593.0
33	182723.0
34	234519.0
35	321786.0
36	478104.0
37	827844.0
38	2211833.0
39	9395037.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	14.4146376891166	29.039024617388336	25.333809172671995	31.212528520823067
2	23.950096638938717	24.502874148371525	31.94495507210217	19.60207414058759
3	15.151512203055056	25.42525650497164	33.53802069831624	25.885210593657064
4	17.494309735269773	28.8223810361112	32.31778469219751	21.365524536421518
5	23.74854833093676	27.795082360283587	29.44167643712631	19.014692871653345
6	21.286614898867924	27.591178022060504	30.48850444900515	20.63370263006642
7	22.893927905475234	28.493697445409943	28.697900631124202	19.914474017990617
8	21.172302258522542	26.018169927463457	30.052006413406158	22.757521400607843
9	20.50370397140516	25.90768670497011	31.522217920980555	22.066391402644175
10	22.387478707116255	26.8945575701892	28.445054803764926	22.272908918929627
11	21.60289979365623	27.21463018317961	28.432044512986316	22.75042551017784
12	21.718803970640664	27.029789534916855	28.691569234276276	22.5598372601662
13	22.048606501948417	27.394689271583566	27.38385431254305	23.172849913924974
14	22.915292026123442	27.43750091217984	27.29613214897061	22.35107491272611
15	22.06876133274861	27.19568464222743	27.8650613228102	22.870492702213767
16	22.58011718991619	27.757837622953158	27.00827946549389	22.653765721636766
17	23.033941430061695	26.731894185748313	27.54876947808023	22.685394906109764
18	22.988356762744978	27.12484388693557	26.920612901454692	22.96618644886476
19	22.965463654932616	27.026898359188273	26.793428969163802	23.214209016715305
20	22.842852784250876	26.988451281951487	26.98655394787961	23.18214198591803
21	23.43724654431526	27.00576358661469	27.034682293842145	22.522307575227906
22	22.734155696762926	26.729809203251737	27.805945119090726	22.73008998089461
23	22.586865583263428	27.063072805503797	27.36427632690023	22.98578528433254
24	22.465526551904595	26.90750531149291	27.736056505805635	22.890911630796857
25	22.51612212715474	26.90156311137768	27.670219708505545	22.91209505296203
26	22.67730517402306	26.691007678990537	27.617108254376205	23.014578892610196
27	22.83666733617771	26.524195179381472	28.03555034155488	22.603587142885935
28	23.58683708850264	26.58819719208457	27.078453026386846	22.74651269302594
29	23.120829600635503	26.59933794855792	27.147632745623103	23.132199705183474
30	22.882814948768505	26.54152138392798	27.73604260592232	22.83962106138119
31	22.83928051424008	26.699222510027028	27.52992123631122	22.931575739421667
32	23.547278020601016	26.77164090207463	26.991036660247236	22.690044417077118
33	22.840705252279406	27.277874652242296	26.973446357917823	22.90797373756047
34	22.886943214111717	27.35438655992483	26.760229097876724	22.99844112808673
35	22.95423254921775	27.3572638357701	26.89687885070205	22.791624764310104
36	22.842880584017497	27.217896655757574	26.976337533646404	22.96288522657852
37	22.898660815742453	27.374464941366817	26.7259797853997	23.000894457491032
38	23.03193289692333	27.403279399469444	26.864158525389183	22.700629178218048
39	22.842136940260385	27.247538156917173	26.953875322216668	22.95644958060577
40	22.946511164038778	27.282440763909786	26.80700915515814	22.964038916893294
41	22.99652989413154	27.22371375692301	26.991231258613585	22.788525090331866
42	22.861270129637262	27.112354841781105	27.055983865015452	22.97039116356618
43	22.937955785861178	27.135185400118566	26.854254858530464	23.072603955489793
44	23.027269486072665	27.065831932340927	26.994386532125063	22.912512049461345
45	22.957519871620676	26.953930921749915	27.04077739267379	23.047771813955624
46	23.14597448954416	27.030936275289967	26.75145827150781	23.07163096365806
47	23.086851335883036	26.991773354062694	26.955390409497515	22.96598490055676
48	22.941006810247828	26.9011252650534	27.013464121968696	23.144403802730075
49	23.105032383253143	26.972688814277408	26.75786611771394	23.16441268475551
50	23.19083636292873	26.973689605875762	26.902174706243343	22.933299324952166
51	23.0455547825676	26.88722538174292	26.939280444740664	23.12793939094881
52	23.111294280684515	26.92693039841931	26.70934162507706	23.252433695819125
53	23.310945254614587	26.92269093400961	26.766928841632375	22.99943496974343
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	672.0
1	656.0
2	640.0
3	636.5
4	633.0
5	719.5
6	806.0
7	972.0
8	1468.5
9	1799.0
10	2610.0
11	3421.0
12	5216.0
13	7011.0
14	9861.5
15	12712.0
16	18941.0
17	25170.0
18	35851.0
19	46532.0
20	63000.0
21	79468.0
22	107959.5
23	136451.0
24	153680.5
25	200102.0
26	229294.0
27	263159.0
28	297024.0
29	333532.0
30	370040.0
31	408476.5
32	446913.0
33	493883.5
34	540854.0
35	592192.0
36	643530.0
37	690956.5
38	738383.0
39	781753.5
40	825124.0
41	868205.0
42	940195.5
43	969105.0
44	988930.5
45	1008756.0
46	1012895.0
47	1017034.0
48	1010255.5
49	1003477.0
50	982156.5
51	960836.0
52	923306.5
53	885777.0
54	838280.5
55	790784.0
56	725769.0
57	660754.0
58	591216.5
59	454231.5
60	386784.0
61	331982.0
62	277180.0
63	232502.5
64	187825.0
65	149905.0
66	111985.0
67	86980.5
68	61976.0
69	46340.5
70	30705.0
71	22149.5
72	13594.0
73	9685.5
74	5777.0
75	4113.0
76	1798.0
77	1147.0
78	828.5
79	510.0
80	401.0
81	292.0
82	232.5
83	173.0
84	124.5
85	76.0
86	64.5
87	53.0
88	43.5
89	34.0
90	24.5
91	15.0
92	12.5
93	8.0
94	6.0
95	4.5
96	3.0
97	3.0
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	1.438861E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.61847677585139
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.99819621037187	41.89665153591834
2	14.578912165378147	16.508715997052796
3	4.89494084088859	8.314322829570512
4	2.2037806507186612	4.990988143671408
5	1.1821129548227478	3.346471743953241
6	0.712807357139433	2.4214840077512996
7	0.4885274521956101	1.93617761445521
8	0.32877349216885304	1.4891723464702211
9	0.24937097638741704	1.2707104351646121
>10	1.2552305022218992	12.425398998750694
>50	0.08519978548880622	3.3220670795911533
>100	0.021862022176919467	1.8820673390233718
>500	2.1183010282491436E-4	0.07481471623915259
>1k	4.917954907508888E-5	0.03225694567103378
>5k	2.458038915696655E-5	0.08870026671696075
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.1814900813907668E-4	6.9499416552398045E-6	0.0	0.0	1.3899883310479609E-5
2	1.1814900813907668E-4	1.3899883310479609E-5	0.0	0.0	1.3899883310479609E-5
3	1.1814900813907668E-4	2.0849824965719414E-5	0.0	0.0	1.3899883310479609E-5
4	1.3204889144955627E-4	2.0849824965719414E-5	0.0	0.0	1.3899883310479609E-5
5	2.4324795793339316E-4	2.0849824965719414E-5	0.0	0.0	1.3899883310479609E-5
6	2.4324795793339316E-4	2.0849824965719414E-5	0.0	0.0	2.7799766620959218E-5
7	2.5019789958863293E-4	2.0849824965719414E-5	0.0	0.0	2.7799766620959218E-5
8	2.5019789958863293E-4	2.0849824965719414E-5	0.0	0.0	5.5599533241918436E-5
9	2.5019789958863293E-4	3.474970827619902E-5	0.0	0.0	5.5599533241918436E-5
10	3.4749708276199024E-4	3.474970827619902E-5	0.0	0.0	5.5599533241918436E-5
11	4.656460909010669E-4	3.474970827619902E-5	0.0	0.0	6.949941655239804E-5
12	7.436437571106591E-4	3.474970827619902E-5	0.0	0.0	7.644935820763784E-5
13	0.001494237455876558	3.474970827619902E-5	0.0	0.0	8.339929986287766E-5
14	0.0022587310379529363	3.474970827619902E-5	0.0	0.0	8.339929986287766E-5
15	0.0025089289375415692	3.474970827619902E-5	0.0	0.0	1.0424912482859707E-4
16	0.0027938765454064013	3.474970827619902E-5	0.0	6.9499416552398045E-6	1.1814900813907668E-4
17	0.00284252613699308	3.474970827619902E-5	0.0	6.9499416552398045E-6	1.3899883310479607E-4
18	0.0030927240365817127	4.169964993143883E-5	0.0	1.3899883310479609E-5	1.3899883310479607E-4
19	0.003148323569823631	4.864959158667863E-5	0.0	1.3899883310479609E-5	1.4594877476003588E-4
20	0.00319002321975507	4.864959158667863E-5	0.0	1.3899883310479609E-5	1.4594877476003588E-4
21	0.0033637717611360654	5.5599533241918436E-5	0.0	1.3899883310479609E-5	1.4594877476003588E-4
22	0.0034610709443094224	6.254947489715823E-5	0.0	1.3899883310479609E-5	1.4594877476003588E-4
23	0.0036000697774142185	6.254947489715823E-5	0.0	1.3899883310479609E-5	1.5289871641527568E-4
24	0.0037043189022428155	6.254947489715823E-5	0.0	1.3899883310479609E-5	1.5289871641527568E-4
25	0.0039336669768657295	6.254947489715823E-5	0.0	1.3899883310479609E-5	1.5289871641527568E-4
26	0.0042325144680410405	6.254947489715823E-5	0.0	1.3899883310479609E-5	1.5289871641527568E-4
27	0.004906658808599302	6.254947489715823E-5	0.0	1.3899883310479609E-5	1.598486580705155E-4
28	0.00499005810846218	6.254947489715823E-5	0.0	1.3899883310479609E-5	1.598486580705155E-4
29	0.005553003382536604	6.949941655239804E-5	0.0	1.3899883310479609E-5	1.598486580705155E-4
30	0.005768451573849038	6.949941655239804E-5	0.0	1.3899883310479609E-5	1.598486580705155E-4
31	0.005949150056885273	6.949941655239804E-5	0.0	1.3899883310479609E-5	1.598486580705155E-4
32	0.006129848539921507	6.949941655239804E-5	0.0	1.3899883310479609E-5	1.6679859972575532E-4
33	0.006247997548060584	6.949941655239804E-5	0.0	1.3899883310479609E-5	1.8764842469147473E-4
34	0.00638004643951014	6.949941655239804E-5	0.0	1.3899883310479609E-5	1.8764842469147473E-4
35	0.006553794980891136	6.949941655239804E-5	0.0	1.3899883310479609E-5	2.1544819131243394E-4
36	0.006852642472066447	6.949941655239804E-5	0.0	1.3899883310479609E-5	2.2934807462291355E-4
37	0.007019441071792202	6.949941655239804E-5	0.0	1.3899883310479609E-5	2.2934807462291355E-4
38	0.00710284037165508	6.949941655239804E-5	0.0	1.3899883310479609E-5	2.4324795793339316E-4
39	0.007172339788207478	6.949941655239804E-5	0.0	1.3899883310479609E-5	2.4324795793339316E-4
40	0.0072487891464151155	6.949941655239804E-5	0.0	1.3899883310479609E-5	2.4324795793339316E-4
41	0.007366938154554192	7.644935820763784E-5	0.0	1.3899883310479609E-5	2.5714784124387274E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCTCGTA	9685	0.0	41.152298	10
CTCGTAT	9740	0.0	40.847538	11
TATGCCG	9835	0.0	40.357395	15
TCGTATG	10145	0.0	39.24002	12
ATGCCGT	10085	0.0	39.217155	16
CGTATGC	10385	0.0	38.378433	13
GAGCTCG	10425	0.0	38.186092	8
TGCCGTC	10335	0.0	38.0866	17
GCCGTCT	10710	0.0	36.79692	18
AGCTCGT	10905	0.0	36.505272	9
CCGTCTT	11000	0.0	35.955	19
ATCGGAA	11460	0.0	35.024433	1
GTATGCC	11430	0.0	34.95188	14
CGGAAGA	12185	0.0	32.61264	3
TCGGAAG	12390	0.0	32.300644	2
CGTCTTC	12645	0.0	31.35192	20
GCTTGAA	13225	0.0	29.852552	28
TGCTTGA	14145	0.0	27.960764	27
AGAGCTC	14440	0.0	27.828947	7
CTTGAAA	15130	0.0	26.109385	29
>>END_MODULE