FastQCFastQC Report
Thu 26 May 2016
SRR805225_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805225_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15439924
Sequences flagged as poor quality0
Sequence length53
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1502430.973081214648466No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGG200240.12968975754025733No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCAGTG160400.10388652172122091No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT912700.043.5626681
TCAACGC962850.041.27906812
CAACGCA962850.041.25954413
AGTGGTA960700.041.109715
ATCAACG969550.041.03259311
GTGGTAT963100.041.0194666
GGTATCA965600.040.9838458
GTATCAA966250.040.975739
AACGCAG973200.040.81833314
TGGTATC970150.040.7334947
TATCAAC972650.040.7012810
AGCAGTG978750.040.6468962
CGCAGAG977750.040.5851216
ACGCAGA978700.040.56733315
GCAGTGG979200.040.553823
AGAGTAC971400.040.2359519
CAGTGGT990400.039.860314
CAGAGTA986650.039.7474318
GCAGAGT1014800.038.86716517
GAGTACT276950.038.7183620
Produced by FastQC (version 0.11.5)