##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805225_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	15439924
Sequences flagged as poor quality	0
Sequence length	53
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.81705512281019	35.0	28.0	37.0	19.0	37.0
2	34.94157374090702	35.0	33.0	37.0	33.0	37.0
3	37.592984395519046	39.0	37.0	39.0	35.0	39.0
4	38.08503319057788	39.0	39.0	39.0	37.0	39.0
5	38.11300800444354	39.0	39.0	39.0	37.0	39.0
6	38.13451491082469	39.0	39.0	39.0	37.0	39.0
7	38.086924067760954	39.0	39.0	39.0	37.0	39.0
8	39.030937457982304	40.0	40.0	40.0	37.0	40.0
9	39.10400336167458	40.0	40.0	40.0	37.0	40.0
10	38.89033216743813	40.0	39.0	40.0	37.0	40.0
11	39.065600517204615	40.0	40.0	40.0	37.0	40.0
12	39.136978070617445	40.0	40.0	40.0	37.0	40.0
13	39.17919323955221	40.0	40.0	40.0	38.0	40.0
14	39.15388178076524	40.0	40.0	40.0	38.0	40.0
15	39.13287766183305	40.0	40.0	40.0	37.0	40.0
16	39.074115844093534	40.0	40.0	40.0	37.0	40.0
17	39.01875922446251	40.0	40.0	40.0	37.0	40.0
18	38.99688709607638	40.0	40.0	40.0	37.0	40.0
19	38.97310226397487	40.0	40.0	40.0	37.0	40.0
20	38.94487485819231	40.0	40.0	40.0	37.0	40.0
21	38.89105257253857	40.0	40.0	40.0	37.0	40.0
22	38.90777817300137	40.0	40.0	40.0	37.0	40.0
23	38.6502093533621	40.0	39.0	40.0	36.0	40.0
24	38.747041047611376	40.0	39.0	40.0	36.0	40.0
25	38.813824472193	40.0	39.0	40.0	37.0	40.0
26	38.83506997832373	40.0	40.0	40.0	37.0	40.0
27	38.85292162059865	40.0	40.0	40.0	37.0	40.0
28	38.83294302484909	40.0	40.0	40.0	37.0	40.0
29	38.79161995875109	40.0	40.0	40.0	36.0	40.0
30	38.68518510842411	40.0	39.0	40.0	36.0	40.0
31	38.69144640867403	40.0	39.0	40.0	36.0	40.0
32	38.66775535941757	40.0	39.0	40.0	36.0	40.0
33	38.58238427857547	40.0	39.0	40.0	35.0	40.0
34	38.56889904380358	40.0	39.0	40.0	35.0	40.0
35	38.53806106817624	40.0	39.0	40.0	35.0	40.0
36	38.4613031126319	40.0	39.0	40.0	35.0	40.0
37	38.40962805257332	40.0	38.0	40.0	35.0	40.0
38	38.23339791050785	40.0	38.0	40.0	35.0	40.0
39	38.2245086180476	40.0	38.0	40.0	35.0	40.0
40	38.2045079366971	40.0	38.0	40.0	35.0	40.0
41	38.16126821608707	40.0	38.0	40.0	35.0	40.0
42	38.11690627492726	40.0	38.0	40.0	35.0	40.0
43	37.99865970842862	40.0	38.0	40.0	34.0	40.0
44	37.8525867096237	40.0	38.0	40.0	34.0	40.0
45	37.76957619739579	40.0	38.0	40.0	34.0	40.0
46	37.682737363215	40.0	37.0	40.0	34.0	40.0
47	37.78683735748958	40.0	38.0	40.0	34.0	40.0
48	37.77440931704068	40.0	38.0	40.0	34.0	40.0
49	37.69354279205001	40.0	37.0	40.0	34.0	40.0
50	37.59257998938337	40.0	37.0	40.0	34.0	40.0
51	37.509572326910416	40.0	37.0	40.0	33.0	40.0
52	37.41150442191296	40.0	37.0	40.0	33.0	40.0
53	36.99362367327715	39.0	36.0	40.0	33.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	6.0
11	9.0
12	30.0
13	54.0
14	165.0
15	433.0
16	878.0
17	1591.0
18	2492.0
19	3696.0
20	5311.0
21	7616.0
22	11253.0
23	16139.0
24	22785.0
25	31655.0
26	42979.0
27	57067.0
28	74334.0
29	94814.0
30	119833.0
31	151680.0
32	183878.0
33	220988.0
34	286681.0
35	397262.0
36	607635.0
37	1112000.0
38	2493216.0
39	9493444.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	15.018273406008994	34.01703272632689	27.908952142510547	23.05574172515357
2	26.473601813065915	36.21703707867992	19.08175195681015	18.22760915144401
3	29.56899917383013	26.119584526452332	22.074428604700387	22.236987695017152
4	21.79938839077187	25.60037212618404	22.946000252332848	29.654239230711237
5	22.63324612219594	30.99530153127697	24.634946389632486	21.736505956894607
6	28.44953122826252	24.9693262738858	23.968356320924897	22.61278617692678
7	22.957489946194034	24.986211072023412	30.25840023564883	21.797898746133722
8	27.194363132875527	26.149066536856008	24.97901544074958	21.677554889518884
9	27.373658056866084	25.35668569353062	25.539426230336364	21.730230019266934
10	21.76570299180229	26.132434330635306	30.325958858346713	21.775903819215692
11	21.831156681859316	31.247420647925466	25.190000935237766	21.731421734977452
12	21.511070909416393	25.58610392123692	30.54283816422931	22.359987005117382
13	21.609335641807565	25.790981872708702	25.35871938229748	27.24096310318626
14	22.01240757402692	31.21353447076553	24.867447534068173	21.906610421139376
15	21.975833559802496	30.885903324394604	24.90315366837298	22.235109447429924
16	22.07487549809183	25.41240487971314	25.264055703901136	27.248663918293897
17	27.628523301021428	25.44846075667212	24.97423562447587	21.94878031783058
18	21.933974545470562	25.49011899281369	25.19717713636414	27.378729325351603
19	22.515907461720666	30.781531048987027	24.917933533869725	21.78462795542258
20	27.64354928171926	25.49763198316261	24.971554264127207	21.88726447099092
21	22.16468163962465	30.717398608956888	25.011632181609183	22.106287569809282
22	27.501022673427666	25.159411406429204	25.38061715847824	21.95894876166489
23	22.056105975651175	25.506880733350762	30.676070685322028	21.76094260567604
24	22.055937581039906	30.465894780311093	25.079974486921046	22.39819315172795
25	21.938307468352825	25.396815424739138	25.385856821575025	27.27902028533301
26	22.286942604121627	29.10149039593718	26.82415405671686	21.787412943224332
27	22.39500660754548	25.12651616678942	30.381859392572142	22.09661783309296
28	26.234028094956948	25.185072154500244	26.68739172550331	21.8935080250395
29	26.551730435978833	25.2767954039152	26.340744941490644	21.830729218615325
30	26.20781682604137	25.15516915756839	26.345641338649077	22.29137267774116
31	24.49525658286919	25.85786691696151	27.071499833807472	22.57537666636183
32	23.863679639873876	26.452578393520586	27.254123789728496	22.429618176877035
33	23.94994949457005	26.095782595821067	27.09736783678469	22.856900072824192
34	23.26595649045941	26.301301742158834	27.345957143312365	23.08678462406939
35	23.126027045210844	26.422927988505645	27.448315160100528	23.002729806182984
36	23.2112476719445	26.419657247017536	27.41975932005883	22.949335760979135
37	23.482563774277647	26.421509587741493	27.31821736946374	22.777709268517125
38	23.476378510671424	26.46751370019697	27.41277094369117	22.64333684544043
39	23.439415893497923	26.278730387532995	27.39878123752423	22.88307248144486
40	23.193210018391284	26.58693138644983	27.325879324276464	22.893979270882422
41	23.370510113909887	26.703272632689124	27.219460406670397	22.706756846730592
42	23.362783391938976	26.614062349011565	27.22859905268964	22.794555206359824
43	23.20815180178348	26.471509833856693	27.562901216353136	22.757437148006687
44	23.351772975048323	26.30825125823158	27.417220447458163	22.922755319261935
45	23.434700844382395	26.07619700718734	27.40672169111713	23.082380457313132
46	23.456935409785697	26.2336524454395	27.166895381091255	23.142516763683552
47	23.31631943266042	26.270854701098266	27.32300366245326	23.08982220378805
48	23.243767262066832	26.170433222339696	27.31830156676937	23.2674979488241
49	23.083099372768935	26.48734540403178	27.27191532808063	23.157639895118653
50	23.53853555237707	26.384682981600164	27.118630894815286	22.958150571207476
51	23.40863853993064	26.245064418710868	27.27062646163284	23.075670579725653
52	23.4637618682579	26.225303958750057	27.266105713991855	23.044828459000186
53	23.80079720599661	26.290777078954537	26.856181416437025	23.052244298611832
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	434.0
1	330.5
2	227.0
3	246.0
4	265.0
5	313.5
6	362.0
7	432.5
8	685.0
9	867.0
10	1261.0
11	1655.0
12	2619.0
13	3583.0
14	5191.5
15	6800.0
16	10522.5
17	14245.0
18	20227.0
19	26209.0
20	37645.0
21	49081.0
22	149670.5
23	250260.0
24	197290.5
25	160561.0
26	176801.0
27	203648.5
28	230496.0
29	265729.0
30	300962.0
31	332169.5
32	363377.0
33	409815.0
34	456253.0
35	514970.0
36	573687.0
37	629424.0
38	685161.0
39	743895.5
40	802630.0
41	862525.0
42	968533.0
43	1014646.0
44	1055612.0
45	1096578.0
46	1114678.0
47	1132778.0
48	1151115.0
49	1169452.0
50	1151703.5
51	1133955.0
52	1093573.5
53	1053192.0
54	1003191.5
55	953191.0
56	900297.5
57	847404.0
58	746873.5
59	561035.5
60	475728.0
61	412671.5
62	349615.0
63	294831.5
64	240048.0
65	194203.5
66	148359.0
67	117295.5
68	86232.0
69	65373.5
70	44515.0
71	32437.0
72	20359.0
73	14679.5
74	9000.0
75	6534.0
76	2973.0
77	1878.0
78	1400.5
79	923.0
80	722.5
81	522.0
82	407.5
83	293.0
84	198.0
85	103.0
86	77.5
87	52.0
88	43.0
89	34.0
90	29.5
91	25.0
92	20.5
93	10.5
94	5.0
95	5.0
96	5.0
97	5.0
98	5.0
99	3.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	1.5439924E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.41592429641769
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.72484612122568	39.91496723472852
2	13.909630280236659	14.859915160805615
3	4.667037743673194	7.478824044137141
4	2.1666828536981138	4.629414691499387
5	1.1938379307936822	3.1884978266733635
6	0.7850090830651246	2.5159191451804177
7	0.4899402667501215	1.8319428538943825
8	0.37693383646343115	1.610741541863114
9	0.2750369975439961	1.3222219895571703
>10	1.2378012154158777	11.586911007823666
>50	0.11612770541630964	4.377729117747375
>100	0.0560000448209871	4.73414088684385
>500	7.153372282657084E-4	0.24910732017845
>1k	3.1562706907799746E-4	0.29935266369466973
>5k	3.640997123854709E-5	0.1217749355640232
>10k+	4.854662831806279E-5	1.2785395798088781
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	150243	0.973081214648466	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGG	20024	0.12968975754025733	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCAGTG	16040	0.10388652172122091	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.044056434474677E-4	0.0	0.0	0.0	1.2953431636062458E-5
2	4.274632439900611E-4	0.0	0.0	1.9430147454093686E-5	1.2953431636062458E-5
3	4.339399598080923E-4	0.0	0.0	2.5906863272124916E-5	1.9430147454093686E-5
4	4.468933914441548E-4	0.0	0.0	5.8290442362281055E-5	3.238357909015614E-5
5	4.5984682308021724E-4	6.476715818031229E-6	0.0	6.476715818031228E-5	3.238357909015614E-5
6	4.922304021703734E-4	6.476715818031229E-6	0.0	8.419730563440597E-5	3.238357909015614E-5
7	5.051838338064359E-4	6.476715818031229E-6	0.0	8.419730563440597E-5	3.238357909015614E-5
8	5.37567412896592E-4	2.5906863272124916E-5	0.0	1.1010416890653088E-4	4.53370107262186E-5
9	5.505208445326544E-4	3.238357909015614E-5	0.0	1.2953431636062457E-4	6.476715818031228E-5
10	6.088112868949354E-4	3.238357909015614E-5	0.0	1.360110321786558E-4	6.476715818031228E-5
11	6.671017292572166E-4	3.238357909015614E-5	0.0	1.4248774799668703E-4	7.124387399834351E-5
12	8.484497721620909E-4	3.886029490818737E-5	0.0	1.4896446381471826E-4	7.772058981637474E-5
13	0.0026165931904846163	3.886029490818737E-5	0.0	1.4896446381471826E-4	7.772058981637474E-5
14	0.0028108946650255533	3.886029490818737E-5	0.0	1.4896446381471826E-4	9.06740214524372E-5
15	0.004274632439900611	3.886029490818737E-5	0.0	1.7487132708684318E-4	9.715073727046843E-5
16	0.004514270925167766	3.886029490818737E-5	0.0	1.7487132708684318E-4	1.1010416890653088E-4
17	0.00458551479916611	3.886029490818737E-5	0.0	1.813480429048744E-4	1.2305760054259334E-4
18	0.005654172909141263	3.886029490818737E-5	0.0	1.9430147454093687E-4	1.2953431636062457E-4
19	0.005867904531136293	3.886029490818737E-5	0.0	1.9430147454093687E-4	1.2953431636062457E-4
20	0.006049252574041168	3.886029490818737E-5	0.0	2.007781903589681E-4	1.2953431636062457E-4
21	0.0064378555231230416	3.886029490818737E-5	0.0	2.007781903589681E-4	1.2953431636062457E-4
22	0.006606250134391853	4.53370107262186E-5	0.0	2.0725490617699933E-4	1.2953431636062457E-4
23	0.0068717954829311334	4.53370107262186E-5	0.0	2.0725490617699933E-4	1.4248774799668703E-4
24	0.007130864115652382	4.53370107262186E-5	0.0	2.1373162199503056E-4	1.6191789545078072E-4
25	0.007415839611645757	5.181372654424983E-5	0.0	2.2020833781306176E-4	1.6191789545078072E-4
26	0.007629571233640787	5.181372654424983E-5	0.0	2.461152010851867E-4	1.7487132708684318E-4
27	0.008652892332889722	5.181372654424983E-5	0.0	2.5259191690321794E-4	1.813480429048744E-4
28	0.00890548424979294	5.181372654424983E-5	0.0	2.655453485392804E-4	1.9430147454093687E-4
29	0.009520772252505905	5.181372654424983E-5	0.0	2.720220643573116E-4	1.9430147454093687E-4
30	0.00990289848576975	6.476715818031228E-5	0.0	2.720220643573116E-4	2.0725490617699933E-4
31	0.010408082319576184	6.476715818031228E-5	0.0	2.8497549599337406E-4	2.0725490617699933E-4
32	0.010900312721746557	6.476715818031228E-5	0.0	3.044056434474677E-4	2.0725490617699933E-4
33	0.011399019839734962	6.476715818031228E-5	0.0	3.2383579090156144E-4	2.0725490617699933E-4
34	0.011968970831721711	6.476715818031228E-5	0.0	3.2383579090156144E-4	2.26685053631093E-4
35	0.01244177108643799	7.772058981637474E-5	0.0	3.367892225376239E-4	2.5259191690321794E-4
36	0.0133355578693263	7.772058981637474E-5	0.0	3.4974265417368636E-4	2.5906863272124914E-4
37	0.014825202507473483	7.772058981637474E-5	0.0	3.6917280162778E-4	2.655453485392804E-4
38	0.016722880242156634	8.419730563440597E-5	0.0	3.756495174458113E-4	2.720220643573116E-4
39	0.017331691529051566	8.419730563440597E-5	0.0	3.8860294908187374E-4	2.720220643573116E-4
40	0.017785061636313753	8.419730563440597E-5	0.0	4.339399598080923E-4	2.7849878017534286E-4
41	0.018380919491572625	8.419730563440597E-5	0.0	4.404166756261235E-4	3.044056434474677E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	91270	0.0	43.562668	1
TCAACGC	96285	0.0	41.279068	12
CAACGCA	96285	0.0	41.259544	13
AGTGGTA	96070	0.0	41.10971	5
ATCAACG	96955	0.0	41.032593	11
GTGGTAT	96310	0.0	41.019466	6
GGTATCA	96560	0.0	40.983845	8
GTATCAA	96625	0.0	40.97573	9
AACGCAG	97320	0.0	40.818333	14
TGGTATC	97015	0.0	40.733494	7
TATCAAC	97265	0.0	40.70128	10
AGCAGTG	97875	0.0	40.646896	2
CGCAGAG	97775	0.0	40.58512	16
ACGCAGA	97870	0.0	40.567333	15
GCAGTGG	97920	0.0	40.55382	3
AGAGTAC	97140	0.0	40.23595	19
CAGTGGT	99040	0.0	39.86031	4
CAGAGTA	98665	0.0	39.74743	18
GCAGAGT	101480	0.0	38.867165	17
GAGTACT	27695	0.0	38.71836	20
>>END_MODULE