FastQCFastQC Report
Thu 26 May 2016
SRR805224_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805224_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26636804
Sequences flagged as poor quality0
Sequence length53
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT444190.16675799393951315No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT320850.030.8572561
TCAACGC401500.024.57110612
CAACGCA402250.024.49024213
ATCAACG411800.023.89946611
AACGCAG415700.023.74308414
GGTATCA421800.023.3217168
ACGCAGA426050.023.16629415
CGCAGAG427800.023.1044916
GTATCAA426150.023.10029
GTGGTAT431450.022.7183916
AGTGGTA434200.022.585335
TATCAAC436250.022.56538810
TGGTATC438350.022.4036737
AGAGTAC440250.022.25894419
GCAGTGG462250.021.3419133
AGCAGTG470450.021.0098842
CAGAGTA469000.020.98965818
CAGTGGT478650.020.6008574
GCAGAGT501050.019.88154817
GTACATG326900.019.44554922
Produced by FastQC (version 0.11.5)