##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805224_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	26636804
Sequences flagged as poor quality	0
Sequence length	53
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.31702947545809	37.0	37.0	37.0	35.0	37.0
2	36.31619653018433	37.0	37.0	37.0	35.0	37.0
3	38.15751690780921	39.0	39.0	39.0	37.0	39.0
4	38.13155493429317	39.0	39.0	39.0	37.0	39.0
5	38.106874983950775	39.0	39.0	39.0	37.0	39.0
6	38.08409387252315	39.0	39.0	39.0	37.0	39.0
7	38.055016585323074	39.0	39.0	39.0	37.0	39.0
8	39.029398571990846	40.0	40.0	40.0	37.0	40.0
9	39.006809563189336	40.0	40.0	40.0	37.0	40.0
10	39.01811080638653	40.0	40.0	40.0	37.0	40.0
11	39.02223622623795	40.0	40.0	40.0	37.0	40.0
12	38.93557308902374	40.0	40.0	40.0	37.0	40.0
13	38.884245459778136	40.0	40.0	40.0	37.0	40.0
14	38.82003370224145	40.0	39.0	40.0	36.0	40.0
15	38.80961394617763	40.0	39.0	40.0	36.0	40.0
16	38.750564181798985	40.0	39.0	40.0	36.0	40.0
17	38.68228973716216	40.0	39.0	40.0	36.0	40.0
18	38.64991096529449	40.0	39.0	40.0	36.0	40.0
19	38.6423039716026	40.0	39.0	40.0	36.0	40.0
20	38.55581003636923	40.0	39.0	40.0	36.0	40.0
21	38.57587794691886	40.0	39.0	40.0	36.0	40.0
22	38.455803819407166	40.0	38.0	40.0	36.0	40.0
23	38.463643874092405	40.0	38.0	40.0	36.0	40.0
24	38.235266100242356	40.0	38.0	40.0	35.0	40.0
25	38.18524737427208	40.0	38.0	40.0	35.0	40.0
26	38.14698291131323	40.0	38.0	40.0	35.0	40.0
27	38.13536984391971	40.0	38.0	40.0	35.0	40.0
28	38.095124062181036	40.0	38.0	40.0	35.0	40.0
29	38.03678357959161	40.0	38.0	40.0	34.0	40.0
30	37.93965950269409	40.0	38.0	40.0	34.0	40.0
31	37.90638940767819	40.0	38.0	40.0	34.0	40.0
32	37.824262587959126	40.0	38.0	40.0	34.0	40.0
33	37.730513465504345	40.0	38.0	40.0	34.0	40.0
34	37.62138911259774	39.0	37.0	40.0	34.0	40.0
35	37.526393406656446	39.0	37.0	40.0	33.0	40.0
36	37.41250785942638	39.0	37.0	40.0	33.0	40.0
37	37.29053549367259	39.0	37.0	40.0	33.0	40.0
38	37.185671674424604	39.0	36.0	40.0	33.0	40.0
39	37.06932032836973	38.0	36.0	40.0	33.0	40.0
40	36.98723934748328	38.0	36.0	40.0	33.0	40.0
41	36.8700202546822	38.0	36.0	40.0	33.0	40.0
42	36.74067534528542	38.0	36.0	40.0	32.0	40.0
43	36.57353205737445	38.0	36.0	40.0	32.0	40.0
44	36.34078476531944	38.0	35.0	40.0	31.0	40.0
45	36.04556924321702	38.0	35.0	40.0	31.0	40.0
46	35.965582920533556	38.0	35.0	39.0	31.0	40.0
47	35.832322150960756	38.0	34.0	39.0	30.0	40.0
48	35.65319086328825	38.0	34.0	39.0	30.0	40.0
49	35.40965421377129	37.0	34.0	39.0	30.0	40.0
50	35.21839204132748	37.0	34.0	39.0	29.0	40.0
51	35.02410003842803	37.0	34.0	39.0	29.0	40.0
52	34.92721713160483	37.0	34.0	39.0	29.0	40.0
53	35.33148890535066	37.0	34.0	39.0	29.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	4.0
10	18.0
11	44.0
12	122.0
13	322.0
14	877.0
15	2042.0
16	3673.0
17	5560.0
18	7379.0
19	9800.0
20	13310.0
21	19225.0
22	27253.0
23	38846.0
24	55215.0
25	75876.0
26	102506.0
27	134680.0
28	173438.0
29	218372.0
30	271517.0
31	335412.0
32	411377.0
33	516411.0
34	671764.0
35	930771.0
36	1452205.0
37	2996907.0
38	1.143787E7
39	6724008.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	13.428431579103858	31.243444221010897	15.075502301252058	40.25262189863319
2	22.680791584455854	24.75738080289212	31.468403641818288	21.09342397083374
3	14.906919764097825	24.50512080953856	34.03564481684815	26.552314609515467
4	15.868101143065061	28.62507453972331	32.67376972102209	22.83305459618954
5	22.09304089184273	28.410991048325467	30.164805056943017	19.331163002888786
6	20.999471257888146	26.545801065322998	31.603344755624583	20.851382921164266
7	22.686336543978776	28.10922061070089	28.98753919576838	20.216903649551952
8	20.29701461181304	25.78102463043239	31.262440493987192	22.65952026376738
9	20.587582504267406	25.098412707470462	32.48266195899478	21.831342829267356
10	21.417100189647375	26.902859667398538	29.39038031739844	22.28965982555565
11	21.28931083473828	27.89926298965897	28.614405091541762	22.197021084060985
12	21.541998807364426	27.044922506468872	28.81154585963091	22.6015328265358
13	21.94006458132139	27.58860635082197	27.37066353756254	23.1006655302941
14	22.22200531264937	28.25409910288036	27.305430486330117	22.218465098140154
15	21.842902023831385	28.02149236822856	27.30942871374509	22.826176894194962
16	22.504569241865504	27.2932706191028	27.099579964623384	23.10258017440831
17	23.35865443917371	26.9365198617672	27.04591737056743	22.658908328491663
18	22.15283034706416	27.37241675089849	27.00296552093862	23.471787381098725
19	22.832510236588444	27.80601606709273	26.812863885622313	22.548609810696508
20	23.286911597952965	27.313986317577736	27.035705935291638	22.363396149177657
21	22.69316544131946	27.787402722939287	27.162691890513592	22.356739945227663
22	23.006968103230403	27.037620579405846	27.30775433869619	22.64765697866756
23	22.27084375437834	27.34808575383143	28.206848689504945	22.17422180228529
24	22.256367543193246	27.703973044213566	27.307915769474445	22.731743643118747
25	22.28771139360413	27.131516979289255	27.182082354925164	23.398689272181453
26	22.29542252891901	27.285277918477007	28.170357825210562	22.24894172739342
27	22.50280852012126	26.757549441742334	28.292883785907648	22.446758252228758
28	23.061918389308268	26.78093437936473	27.437465846127786	22.719681385199213
29	23.107922406907374	26.894138651168507	27.599463509210793	22.398475432713326
30	22.955006163652367	26.718318008421733	27.7042320842996	22.6224437436263
31	22.824487502329482	26.92858722840773	27.287613033455514	22.959312235807268
32	22.7949419157043	27.16873991339201	27.49449971550641	22.541818455397276
33	22.735152460482873	27.017704526413905	27.495457037563515	22.751685975539708
34	22.784381339442973	27.144423933141525	27.181331514096062	22.889863213319437
35	22.76512978058479	27.090243258913492	27.477620813668185	22.667006146833533
36	22.63995335176097	26.97004865899077	27.570954082929767	22.81904390631849
37	22.727426308351404	27.05624894037588	27.280033295285726	22.936291455986986
38	22.796334725442286	27.14588431855413	27.50851415958161	22.549266796421975
39	22.676324081522694	27.02323822332439	27.597428730563923	22.703008964588996
40	22.767070704128017	27.050204671701607	27.352448139048512	22.830276485121864
41	22.788311991183328	27.065341622816312	27.54813978433749	22.598206601662874
42	22.64544950663	26.941843323245536	27.647843938034004	22.764863232090455
43	22.70082026357216	27.016833551052144	27.37697810893529	22.905368076440404
44	22.686250197283428	27.06773680506115	27.627469872136313	22.618543125519114
45	22.73270096517585	26.94162182520095	27.54844012066913	22.777237088954067
46	22.7010793036582	26.973543823050246	27.325451657038137	22.999925216253423
47	22.776411164042052	27.02219080036779	27.539553919456704	22.66184411613345
48	22.652244616133377	26.90843466055462	27.561268236234348	22.878052487077653
49	22.7398151820316	26.979355331067495	27.23346614706479	23.047363339836117
50	22.811002401038806	27.083264193406986	27.471122286292303	22.634611119261905
51	22.71557804006817	26.902382883472054	27.46737934475923	22.914659731700546
52	22.892468630996422	27.01425065860003	27.083065220587276	23.01021548981627
53	22.934793528532925	27.042065557114135	27.220390254025972	22.802750660326968
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	284.0
1	440.5
2	597.0
3	667.5
4	738.0
5	905.5
6	1073.0
7	1378.5
8	2248.0
9	2812.0
10	4164.5
11	5517.0
12	8306.5
13	11096.0
14	16302.5
15	21509.0
16	32153.5
17	42798.0
18	60588.5
19	78379.0
20	106338.5
21	134298.0
22	207014.0
23	279730.0
24	294554.5
25	357540.5
26	405702.0
27	470663.0
28	535624.0
29	608158.5
30	680693.0
31	752611.0
32	824529.0
33	914541.0
34	1004553.0
35	1107861.5
36	1211170.0
37	1303857.0
38	1396544.0
39	1487761.0
40	1578978.0
41	1673487.5
42	1832080.0
43	1896163.0
44	1932601.5
45	1969040.0
46	1959023.0
47	1949006.0
48	1931055.5
49	1913105.0
50	1856871.0
51	1800637.0
52	1708648.0
53	1616659.0
54	1507067.5
55	1397476.0
56	1271306.0
57	1145136.0
58	1012608.5
59	763700.0
60	647319.0
61	551989.0
62	456659.0
63	382076.0
64	307493.0
65	244280.0
66	181067.0
67	139253.5
68	97440.0
69	72511.5
70	47583.0
71	34267.0
72	20951.0
73	14772.0
74	8593.0
75	6183.0
76	2669.5
77	1566.0
78	1126.0
79	686.0
80	532.5
81	379.0
82	277.0
83	175.0
84	118.0
85	61.0
86	49.5
87	38.0
88	26.5
89	15.0
90	12.0
91	9.0
92	7.0
93	4.0
94	3.0
95	2.0
96	1.0
97	0.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	2.6636804E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.316565391828284
#Duplication Level	Percentage of deduplicated	Percentage of total
1	59.104648707165495	23.82896434563477
2	19.34136629122546	15.595549176949891
3	8.556000506988973	10.348456617976106
4	4.328569226715976	6.98052177127801
5	2.4053576534777235	4.848787956358464
6	1.441363958852493	3.486650658030061
7	0.9863335017303878	2.783590538446491
8	0.7009855899190658	2.2609065099761074
9	0.5065766669307863	1.8381088186460521
>10	2.4117020595335013	17.002700082551765
>50	0.1354932817176258	3.722762450891246
>100	0.07731568440324595	5.744512130405667
>500	0.003683454778077561	1.0009875526676073
>1k	5.847280588338274E-4	0.3162823815511094
>5k	0.0	0.0
>10k+	1.8688502159452324E-5	0.24121900863671086
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	44419	0.16675799393951315	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.1774384043971642E-4	0.0	0.0	1.877102072756176E-5	0.0
2	2.1774384043971642E-4	0.0	0.0	1.877102072756176E-5	0.0
3	2.2900645287625348E-4	0.0	0.0	3.3787837309611166E-5	0.0
4	2.4026906531279054E-4	0.0	0.0	4.5050449746148225E-5	0.0
5	0.0016443414157344101	0.0	0.0	7.13298787647347E-5	0.0
6	0.0016818834571895337	0.0	0.0	9.760930778332115E-5	0.0
7	0.0016818834571895337	0.0	0.0	1.3139714509293233E-4	0.0
8	0.0016818834571895337	0.0	0.0	1.3515134923844466E-4	1.1262612436537056E-5
9	0.0016893918654805585	0.0	0.0	1.3890555338395702E-4	1.1262612436537056E-5
10	0.0016931460696260707	0.0	0.0	1.426597575294694E-4	1.1262612436537056E-5
11	0.0017757385608273425	0.0	0.0	1.6143077825703114E-4	1.1262612436537056E-5
12	0.001865839460319639	0.0	0.0	1.6518498240254348E-4	1.1262612436537056E-5
13	0.0020986001173414048	0.0	0.0	1.802017989845929E-4	1.1262612436537056E-5
14	0.0021924552209792135	0.0	0.0	1.802017989845929E-4	1.1262612436537056E-5
15	0.0022750477121804853	0.0	0.0	1.802017989845929E-4	1.1262612436537056E-5
16	0.002335114978508683	0.0	0.0	1.952186155666423E-4	1.5016816582049408E-5
17	0.002406444857273418	0.0	0.0	1.9897281971215466E-4	2.6279429018586465E-5
18	0.0024702663277471277	0.0	0.0	2.0648122800317936E-4	2.6279429018586465E-5
19	0.002612926085276597	0.0	0.0	2.3651486116727817E-4	3.3787837309611166E-5
20	0.0027142895972054304	0.0	0.0	2.4026906531279054E-4	3.3787837309611166E-5
21	0.0028306699257163136	0.0	0.0	2.5528588189483993E-4	3.3787837309611166E-5
22	0.0029508044583727087	0.0	0.0	2.5528588189483993E-4	3.754204145512352E-5
23	0.0031535314822303756	0.0	0.0	2.590400860403523E-4	3.754204145512352E-5
24	0.0033149622604874067	0.0	0.0	2.740569026224017E-4	4.5050449746148225E-5
25	0.0034388509972893143	0.0	0.0	2.965821274954758E-4	4.5050449746148225E-5
26	0.0036641032460200556	0.0	0.0	3.0409053578650053E-4	4.5050449746148225E-5
27	0.0038668302698777225	0.0	0.0	3.0409053578650053E-4	4.5050449746148225E-5
28	0.004062048885444365	0.0	0.0	3.228615565140623E-4	4.5050449746148225E-5
29	0.004291055338320618	0.0	0.0	3.2661576065957465E-4	4.5050449746148225E-5
30	0.0045125533829058475	0.0	0.0	3.341241689505993E-4	4.5050449746148225E-5
31	0.004741559835782101	0.0	0.0	3.378783730961117E-4	5.631306218268528E-5
32	0.005053158779859626	0.0	0.0	3.5664939382367344E-4	6.0067266328197634E-5
33	0.005368511928082663	0.0	0.0	3.604035979691858E-4	7.13298787647347E-5
34	0.0055637305436493055	0.0	0.0	3.604035979691858E-4	7.13298787647347E-5
35	0.005822770629689658	0.0	0.0	3.754204145512352E-4	8.259249120127174E-5
36	0.006059285490856936	0.0	0.0	3.7917461869674756E-4	1.238887368019076E-4
37	0.006318325576897288	0.0	0.0	3.904372311332846E-4	1.3890555338395702E-4
38	0.0065886282753741775	0.0	0.0	4.167166601518711E-4	1.426597575294694E-4
39	0.0069415234650523384	0.0	0.0	4.242250684428958E-4	1.5392236996600642E-4
40	0.007275647634002938	0.0	0.0	4.392418850249452E-4	1.5392236996600642E-4
41	0.007692364294154809	0.0	0.0	4.4299608917045756E-4	1.5392236996600642E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	32085	0.0	30.857256	1
TCAACGC	40150	0.0	24.571106	12
CAACGCA	40225	0.0	24.490242	13
ATCAACG	41180	0.0	23.899466	11
AACGCAG	41570	0.0	23.743084	14
GGTATCA	42180	0.0	23.321716	8
ACGCAGA	42605	0.0	23.166294	15
CGCAGAG	42780	0.0	23.10449	16
GTATCAA	42615	0.0	23.1002	9
GTGGTAT	43145	0.0	22.718391	6
AGTGGTA	43420	0.0	22.58533	5
TATCAAC	43625	0.0	22.565388	10
TGGTATC	43835	0.0	22.403673	7
AGAGTAC	44025	0.0	22.258944	19
GCAGTGG	46225	0.0	21.341913	3
AGCAGTG	47045	0.0	21.009884	2
CAGAGTA	46900	0.0	20.989658	18
CAGTGGT	47865	0.0	20.600857	4
GCAGAGT	50105	0.0	19.881548	17
GTACATG	32690	0.0	19.445549	22
>>END_MODULE