FastQCFastQC Report
Thu 26 May 2016
SRR805223_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805223_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27133961
Sequences flagged as poor quality0
Sequence length53
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT614340.22640999594567118No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGTAC525150.024.8715619
GAGTACT207850.022.499420
ACATGGG372100.021.99059324
GTACATG387400.021.53458822
GAGTACA395550.021.34635420
AGTACAT402050.020.88434221
TACATGG399700.020.84250623
GTACTTT232300.019.99978422
CATGGGG253900.019.1313525
AGTACTT251900.018.57423421
TACTTTT281150.016.566623
ATGGGGG147800.016.10656226
ACTTTTT319400.014.70037524
TGGGGGG112200.013.00668427
AAGCAGT1290200.011.4603931
CTTTTTT417700.011.38149425
TCAACGC1357700.010.771193512
GTGGTAT1365150.010.7227416
CGCAGAG1366950.010.69658716
TGGTATC1375300.010.6726547
Produced by FastQC (version 0.11.5)