##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805223_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	27133961
Sequences flagged as poor quality	0
Sequence length	53
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.371707249081695	37.0	37.0	37.0	35.0	37.0
2	36.36544251685185	37.0	37.0	37.0	35.0	37.0
3	38.15213315151444	39.0	39.0	39.0	37.0	39.0
4	38.121064705591635	39.0	39.0	39.0	37.0	39.0
5	38.10212069664285	39.0	39.0	39.0	37.0	39.0
6	38.08320101145572	39.0	39.0	39.0	37.0	39.0
7	38.04648702045382	39.0	39.0	39.0	37.0	39.0
8	39.04178663778576	40.0	40.0	40.0	37.0	40.0
9	39.02398035436109	40.0	40.0	40.0	37.0	40.0
10	39.02332162267057	40.0	40.0	40.0	37.0	40.0
11	39.02260377686841	40.0	40.0	40.0	37.0	40.0
12	38.944807026147046	40.0	40.0	40.0	37.0	40.0
13	38.9016666236087	40.0	40.0	40.0	37.0	40.0
14	38.84710754172603	40.0	39.0	40.0	37.0	40.0
15	38.83117252951016	40.0	39.0	40.0	36.0	40.0
16	38.77316197955765	40.0	39.0	40.0	36.0	40.0
17	38.70710579262644	40.0	39.0	40.0	36.0	40.0
18	38.676032223972015	40.0	39.0	40.0	36.0	40.0
19	38.67418277044033	40.0	39.0	40.0	36.0	40.0
20	38.57628829053009	40.0	39.0	40.0	36.0	40.0
21	38.60550345008604	40.0	39.0	40.0	36.0	40.0
22	38.47511131898509	40.0	38.0	40.0	36.0	40.0
23	38.484535486728234	40.0	38.0	40.0	36.0	40.0
24	38.269749816475375	40.0	38.0	40.0	35.0	40.0
25	38.226380254618924	40.0	38.0	40.0	35.0	40.0
26	38.1779935483802	40.0	38.0	40.0	35.0	40.0
27	38.17823214236948	40.0	38.0	40.0	35.0	40.0
28	38.13230939633178	40.0	38.0	40.0	35.0	40.0
29	38.06629256966943	40.0	38.0	40.0	35.0	40.0
30	37.96201973607908	40.0	38.0	40.0	34.0	40.0
31	37.94027160280801	40.0	38.0	40.0	34.0	40.0
32	37.86408976558933	40.0	38.0	40.0	34.0	40.0
33	37.76509142915035	40.0	38.0	40.0	34.0	40.0
34	37.67174468187671	40.0	37.0	40.0	34.0	40.0
35	37.58670096120504	39.0	37.0	40.0	34.0	40.0
36	37.46187130585173	39.0	37.0	40.0	33.0	40.0
37	37.3413581968368	39.0	37.0	40.0	33.0	40.0
38	37.23259832945142	39.0	37.0	40.0	33.0	40.0
39	37.11912186355689	38.0	36.0	40.0	33.0	40.0
40	37.03267543577585	38.0	36.0	40.0	33.0	40.0
41	36.93146665906979	38.0	36.0	40.0	33.0	40.0
42	36.79315603792605	38.0	36.0	40.0	32.0	40.0
43	36.62776311943546	38.0	36.0	40.0	32.0	40.0
44	36.39109063361593	38.0	35.0	40.0	31.0	40.0
45	36.08864912866942	38.0	35.0	40.0	31.0	40.0
46	36.00433994874541	38.0	35.0	39.0	31.0	40.0
47	35.8789079486036	38.0	35.0	39.0	31.0	40.0
48	35.697970819667646	38.0	34.0	39.0	30.0	40.0
49	35.43492684315423	37.0	34.0	39.0	30.0	40.0
50	35.2562325124592	37.0	34.0	39.0	29.0	40.0
51	35.08574487152834	37.0	34.0	39.0	29.0	40.0
52	34.97361774788428	37.0	34.0	39.0	29.0	40.0
53	35.330681318514465	37.0	34.0	39.0	29.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	12.0
11	43.0
12	112.0
13	291.0
14	841.0
15	1923.0
16	3750.0
17	5584.0
18	7815.0
19	10588.0
20	14510.0
21	20313.0
22	28409.0
23	40477.0
24	56346.0
25	76990.0
26	102588.0
27	134654.0
28	173195.0
29	217944.0
30	272244.0
31	335187.0
32	413389.0
33	518399.0
34	673763.0
35	931177.0
36	1453525.0
37	2968778.0
38	1.1346227E7
39	7324885.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.907035209492633	41.36464263363539	14.173271642868507	21.555050514003465
2	29.011823964809263	28.812627098564786	28.541553516642853	13.633995419983098
3	21.643448960511147	25.806294923177635	28.84677987117325	23.703476245137967
4	20.4251712457315	31.98602666230706	25.94897221235042	21.639829879611018
5	21.935868485990675	30.476331855861368	24.906769048573484	22.681030609574474
6	21.673094466377393	26.524203377457496	30.397246461731115	21.405455694433996
7	21.39710085084887	28.18146233791668	27.33497331996608	23.08646349126838
8	21.21592199531797	26.173583724101324	29.26348644785035	23.347007832730355
9	22.557521181666033	26.481187173520297	28.778024704907622	22.183266939906048
10	21.449242150823462	28.263960429514878	29.002171853936105	21.28462556572555
11	21.543843156552043	29.05547037529832	28.16292099778576	21.237765470363872
12	21.725655904053227	26.849784297987306	29.09691659098353	22.327643206975935
13	22.032761821983897	27.86930371131587	27.624057541764728	22.473876924935507
14	22.018790400708543	28.799204804635785	27.015967185918782	22.16603760873689
15	21.73239653436518	28.32601181965287	27.284070320584597	22.65752132539735
16	23.005926042275952	27.153234280833527	26.85928162128633	22.981558055604193
17	23.265213656052648	27.452442347064625	26.76941637824275	22.512927618639978
18	22.432113763265157	27.48135445466292	26.54953325833998	23.53699852373194
19	23.13737386148672	28.07296361928139	26.511039062818732	22.278623456413165
20	23.49895763467781	27.673408979986373	26.773842565779464	22.053790819556347
21	22.944445891994906	28.016230287940637	26.689538619149634	22.349785200914823
22	23.718929204622942	27.06933204481277	27.269096465495767	21.942642285068516
23	22.470195191922034	27.166306460011498	28.1640339941522	22.19946435391427
24	22.40070662738846	28.43563090549146	26.51859785602257	22.64506461109751
25	22.637387884503852	27.27958516635297	26.994042631667376	23.0889843174758
26	22.14729357059222	27.440564243458592	28.179612257863862	22.232529928085324
27	22.709286712691892	27.043239282314886	27.974426586667533	22.273047418325692
28	23.489441884286634	27.152294499133394	26.92760190817699	22.430661708402987
29	23.181536230556237	26.927292333028706	27.66108494075008	22.23008649566497
30	23.391804093770165	26.93477373244548	27.24443364534946	22.4289885284349
31	23.139305020745034	27.1533927538261	27.11865768510539	22.58864454032347
32	23.078141816449136	26.988543987366974	27.393733631444373	22.539580564739516
33	22.90758433683899	27.0484504639776	27.384881256370935	22.659083942812476
34	22.926177272827953	27.5831051721494	27.00206947301207	22.488648082010585
35	22.778049249794382	26.934762676190182	27.62528478610255	22.661903287912885
36	23.130117272594298	26.92951832576158	27.528413562619917	22.411950839024204
37	22.97764782664794	27.5104029227432	26.81040928746083	22.701539963148026
38	22.621898070834554	27.36223804552531	27.62179469484754	22.3940691887926
39	23.04155298225718	26.844926916494057	27.506297366610056	22.607222734638707
40	22.722904333797782	27.52754011845156	27.124270577377185	22.625284970373475
41	22.871769440517735	27.314386572605454	27.21852146835473	22.595322518522085
42	22.94872834821278	26.88065336277295	27.447010040296	22.72360824871828
43	22.861601371064108	27.4804625834024	27.098365771219324	22.55957027431417
44	22.913860604428525	27.194079773314332	27.310059891366397	22.581999730890743
45	23.15080721167101	26.791982195301305	27.50681701060896	22.550393582418728
46	22.927621956853258	27.03017447397378	27.235994037140394	22.80620953203257
47	22.791633702134384	27.64891937450636	27.050890210979517	22.508556712379736
48	23.059980074416707	26.862793825051934	27.48639242165934	22.590833678872023
49	22.69517893093456	27.11537029186413	27.386860326068867	22.80259045113244
50	22.95105016182488	27.416078323397013	27.180023587415047	22.452847927363056
51	22.945525719595455	27.007310138022238	27.10192220000611	22.9452419423762
52	22.87808624771002	27.161979778772437	27.2103508956912	22.749583077826344
53	23.18039375084235	27.273946476152155	26.956491903264695	22.589167869740802
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1071.0
1	1224.5
2	1378.0
3	1545.5
4	1713.0
5	1955.5
6	2198.0
7	2593.0
8	3757.0
9	4526.0
10	5953.5
11	7381.0
12	10631.5
13	13882.0
14	19359.5
15	24837.0
16	35187.5
17	45538.0
18	62971.0
19	80404.0
20	105630.5
21	130857.0
22	210096.5
23	289336.0
24	295810.5
25	349409.0
26	396533.0
27	459548.5
28	522564.0
29	595938.5
30	669313.0
31	733990.5
32	798668.0
33	881660.5
34	964653.0
35	1074835.5
36	1185018.0
37	1282314.0
38	1379610.0
39	1471482.5
40	1563355.0
41	1655690.5
42	1824182.0
43	1900338.0
44	2003432.0
45	2106526.0
46	2185285.5
47	2264045.0
48	2172940.5
49	2081836.0
50	1963090.0
51	1844344.0
52	1729494.5
53	1614645.0
54	1504186.5
55	1393728.0
56	1273141.5
57	1152555.0
58	1018941.5
59	764052.5
60	642777.0
61	545847.0
62	448917.0
63	373961.5
64	299006.0
65	240032.5
66	181059.0
67	139730.0
68	98401.0
69	72725.0
70	47049.0
71	33795.5
72	20542.0
73	14969.0
74	9396.0
75	6783.0
76	2937.0
77	1704.0
78	1239.5
79	775.0
80	559.5
81	344.0
82	264.0
83	184.0
84	133.5
85	83.0
86	58.5
87	34.0
88	25.5
89	17.0
90	14.5
91	12.0
92	9.0
93	4.0
94	2.0
95	2.5
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	2.7133961E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.12617069067261
#Duplication Level	Percentage of deduplicated	Percentage of total
1	62.92906068924076	26.50960351998652
2	18.061770998851213	15.217464961468929
3	7.553586957038427	9.546110804371173
4	3.8144278293259775	6.427489513017516
5	2.09873002727713	4.420572968135818
6	1.292103425952846	3.2658821683015473
7	0.8631189837044642	2.5451928343725885
8	0.5919543234746811	1.9949415097420837
9	0.45283127828258446	1.716844295070686
>10	2.12838304901463	15.628280708820583
>50	0.12596025540894198	3.6843398540139125
>100	0.0850104343534495	6.560691522293527
>500	0.0024201771426790395	0.6450193910598022
>1k	3.875696317618011E-4	0.3742384293441966
>5k	1.051039864144973E-4	0.2925518615817396
>10k+	1.488973140514998E-4	1.1707756584191944
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	61434	0.22640999594567118	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.542938718014668E-4	0.0	0.0	3.685418431905316E-6	0.0
2	2.542938718014668E-4	7.370836863810632E-6	0.0	3.685418431905316E-6	0.0
3	2.6166470866527744E-4	7.370836863810632E-6	0.0	3.685418431905316E-6	0.0
4	2.6535012709718274E-4	7.370836863810632E-6	0.0	3.685418431905316E-6	3.685418431905316E-6
5	2.6535012709718274E-4	1.1056255295715947E-5	0.0	3.685418431905316E-6	7.370836863810632E-6
6	2.6535012709718274E-4	1.1056255295715947E-5	0.0	3.685418431905316E-6	7.370836863810632E-6
7	2.690355455290881E-4	1.1056255295715947E-5	0.0	3.685418431905316E-6	7.370836863810632E-6
8	2.690355455290881E-4	1.4741673727621264E-5	0.0	3.685418431905316E-6	1.4741673727621264E-5
9	2.727209639609934E-4	1.842709215952658E-5	0.0	1.1056255295715947E-5	1.4741673727621264E-5
10	2.8746263768861463E-4	1.842709215952658E-5	0.0	1.4741673727621264E-5	1.842709215952658E-5
11	3.206314035757625E-4	2.2112510591431895E-5	0.0	1.4741673727621264E-5	1.842709215952658E-5
12	3.5748558789481567E-4	2.5797929023337213E-5	0.0	1.4741673727621264E-5	1.842709215952658E-5
13	4.0171060907767946E-4	2.9483347455242528E-5	0.0	1.4741673727621264E-5	2.2112510591431895E-5
14	4.6804814085197515E-4	2.9483347455242528E-5	0.0	1.4741673727621264E-5	2.2112510591431895E-5
15	5.196439988986495E-4	2.9483347455242528E-5	0.0	1.4741673727621264E-5	2.2112510591431895E-5
16	5.933523675367559E-4	2.9483347455242528E-5	0.0	1.4741673727621264E-5	2.9483347455242528E-5
17	7.076003389258206E-4	2.9483347455242528E-5	0.0	2.2112510591431895E-5	4.053960275095848E-5
18	8.329045656106014E-4	2.9483347455242528E-5	0.0	2.2112510591431895E-5	4.053960275095848E-5
19	0.0010098046503420567	3.316876588714784E-5	0.0	2.2112510591431895E-5	6.633753177429568E-5
20	0.00116459222448208	3.316876588714784E-5	0.0	2.2112510591431895E-5	6.633753177429568E-5
21	0.0013193797986221032	3.316876588714784E-5	0.0	2.2112510591431895E-5	7.370836863810632E-5
22	0.0015220778123768955	3.685418431905316E-5	0.0	2.5797929023337213E-5	7.739378707001163E-5
23	0.0017505737551550252	3.685418431905316E-5	0.0	2.5797929023337213E-5	8.845004236572758E-5
24	0.002085946832458409	4.422502118286379E-5	0.0	2.5797929023337213E-5	8.845004236572758E-5
25	0.0024102636544660765	4.422502118286379E-5	0.0	2.9483347455242528E-5	9.21354607976329E-5
26	0.002749322150201366	4.422502118286379E-5	0.0	2.9483347455242528E-5	1.0319171609334885E-4
27	0.0029999306035709272	4.791043961476911E-5	0.0	2.9483347455242528E-5	1.0319171609334885E-4
28	0.003346359936170027	4.791043961476911E-5	0.0	2.9483347455242528E-5	1.1056255295715947E-4
29	0.0036743621766096002	6.265211334239037E-5	0.0	2.9483347455242528E-5	1.1793338982097011E-4
30	0.003983937324889646	6.265211334239037E-5	0.0	2.9483347455242528E-5	1.2161880825287542E-4
31	0.004393018770831137	6.265211334239037E-5	0.0	3.316876588714784E-5	1.2161880825287542E-4
32	0.004857381493251206	6.265211334239037E-5	0.0	3.685418431905316E-5	1.2530422668478075E-4
33	0.005380710910581761	6.265211334239037E-5	0.0	4.053960275095848E-5	1.2898964511668607E-4
34	0.005859815306729453	6.265211334239037E-5	0.0	4.053960275095848E-5	1.3267506354859137E-4
35	0.006298380100126185	6.265211334239037E-5	0.0	4.053960275095848E-5	1.4373131884430732E-4
36	0.006829080354320551	6.265211334239037E-5	0.0	4.053960275095848E-5	1.4373131884430732E-4
37	0.007374522282242537	6.265211334239037E-5	0.0	4.053960275095848E-5	1.4373131884430732E-4
38	0.007960503812915482	6.633753177429568E-5	0.0	4.422502118286379E-5	1.4741673727621264E-4
39	0.008524372832996997	7.002295020620101E-5	0.0	4.422502118286379E-5	1.5110215570811797E-4
40	0.009114039782101846	7.739378707001163E-5	0.0	4.422502118286379E-5	1.584729925719286E-4
41	0.009762673426117183	7.739378707001163E-5	0.0	4.791043961476911E-5	1.584729925719286E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGAGTAC	52515	0.0	24.87156	19
GAGTACT	20785	0.0	22.4994	20
ACATGGG	37210	0.0	21.990593	24
GTACATG	38740	0.0	21.534588	22
GAGTACA	39555	0.0	21.346354	20
AGTACAT	40205	0.0	20.884342	21
TACATGG	39970	0.0	20.842506	23
GTACTTT	23230	0.0	19.999784	22
CATGGGG	25390	0.0	19.13135	25
AGTACTT	25190	0.0	18.574234	21
TACTTTT	28115	0.0	16.5666	23
ATGGGGG	14780	0.0	16.106562	26
ACTTTTT	31940	0.0	14.700375	24
TGGGGGG	11220	0.0	13.006684	27
AAGCAGT	129020	0.0	11.460393	1
CTTTTTT	41770	0.0	11.381494	25
TCAACGC	135770	0.0	10.7711935	12
GTGGTAT	136515	0.0	10.722741	6
CGCAGAG	136695	0.0	10.696587	16
TGGTATC	137530	0.0	10.672654	7
>>END_MODULE