FastQCFastQC Report
Thu 26 May 2016
SRR805222_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805222_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7020820
Sequences flagged as poor quality0
Sequence length46
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT2495973.555097552707519No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA192330.2739423600092297No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT134630.1917582276714116No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT579900.037.70651
TCAACGC626500.035.45091212
CAACGCA627250.035.40215313
GTGGTAT620150.035.4011156
AGTGGTA621050.035.3465925
AGCAGTG624600.035.264172
GCAGTGG626000.035.2396163
GGTATCA626600.035.237798
ACGCAGA629350.035.2236415
TGGTATC623850.035.1847427
AACGCAG632250.035.1316714
ATCAACG632800.035.1074611
CAGTGGT627150.035.0538184
GTATCAA630050.035.0289659
TATCAAC631250.034.9528710
CGCAGAG634750.034.9365916
AGAGTAC622250.034.9023719
GAGTACT317750.034.7946520
GTACTTT318750.034.5725522
CAGAGTA632450.034.5007518
Produced by FastQC (version 0.11.5)