##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805222_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7020820
Sequences flagged as poor quality	0
Sequence length	46
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.86362547394749	35.0	35.0	35.0	32.0	37.0
2	34.64944223039474	35.0	35.0	37.0	32.0	37.0
3	36.986809802843545	39.0	37.0	39.0	33.0	39.0
4	37.139159813241186	39.0	37.0	39.0	33.0	39.0
5	37.26869154885042	39.0	37.0	39.0	34.0	39.0
6	37.342560555604614	39.0	37.0	39.0	34.0	39.0
7	37.12770061047	39.0	37.0	39.0	33.0	39.0
8	38.15789537404463	40.0	38.0	40.0	34.0	40.0
9	38.23202175814221	40.0	38.0	40.0	34.0	40.0
10	37.95103406724571	40.0	38.0	40.0	34.0	40.0
11	38.15167914858948	40.0	38.0	40.0	34.0	40.0
12	38.21581510421859	40.0	38.0	40.0	34.0	40.0
13	38.2641093205637	40.0	38.0	40.0	34.0	40.0
14	38.1702691423509	40.0	38.0	40.0	34.0	40.0
15	38.15002976860253	40.0	38.0	40.0	34.0	40.0
16	38.13050555348236	40.0	38.0	40.0	34.0	40.0
17	38.16885962038622	40.0	38.0	40.0	34.0	40.0
18	38.11001122376019	40.0	38.0	40.0	34.0	40.0
19	38.00928865858974	40.0	38.0	40.0	34.0	40.0
20	37.90301332323005	40.0	38.0	40.0	34.0	40.0
21	37.72157867599511	40.0	38.0	40.0	33.0	40.0
22	37.669716357918304	40.0	38.0	40.0	33.0	40.0
23	37.218260117764025	40.0	37.0	40.0	32.0	40.0
24	37.34497437621246	40.0	37.0	40.0	32.0	40.0
25	37.41486322110523	40.0	37.0	40.0	33.0	40.0
26	37.37683447232659	40.0	37.0	40.0	32.0	40.0
27	37.300012534148436	40.0	37.0	40.0	32.0	40.0
28	37.227780230799254	40.0	37.0	40.0	32.0	40.0
29	37.059805549779085	40.0	37.0	40.0	31.0	40.0
30	37.08100136451298	40.0	37.0	40.0	31.0	40.0
31	36.93968468070681	39.0	36.0	40.0	31.0	40.0
32	36.879438014363	39.0	36.0	40.0	31.0	40.0
33	36.820098079711485	39.0	36.0	40.0	31.0	40.0
34	36.653897977729095	39.0	36.0	40.0	30.0	40.0
35	36.64993690195732	39.0	36.0	40.0	30.0	40.0
36	36.52610507034791	39.0	36.0	40.0	30.0	40.0
37	36.30909437928903	38.0	35.0	40.0	30.0	40.0
38	36.180631322267196	38.0	35.0	40.0	30.0	40.0
39	36.118202574628036	38.0	35.0	40.0	30.0	40.0
40	36.041579188755726	38.0	35.0	40.0	30.0	40.0
41	35.69014531066172	38.0	35.0	40.0	29.0	40.0
42	35.64222526713404	38.0	34.0	40.0	29.0	40.0
43	35.52765218307833	38.0	34.0	40.0	28.0	40.0
44	35.3964697855806	38.0	34.0	40.0	28.0	40.0
45	35.26174663358411	38.0	34.0	40.0	28.0	40.0
46	35.48508507553249	38.0	34.0	40.0	28.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	5.0
11	8.0
12	14.0
13	47.0
14	117.0
15	271.0
16	525.0
17	858.0
18	1633.0
19	2710.0
20	4520.0
21	7278.0
22	11246.0
23	17205.0
24	24662.0
25	34520.0
26	46140.0
27	61082.0
28	78024.0
29	97346.0
30	120259.0
31	147291.0
32	180466.0
33	221026.0
34	278301.0
35	363282.0
36	511745.0
37	823079.0
38	1710018.0
39	2277141.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	9.908814070151351	50.286547725194495	25.00548368993935	14.799154514714804
2	28.897650132035857	45.26521118615774	15.343692047367686	10.493446634438712
3	35.79900923253979	25.72403223555083	21.833104964947115	16.643853566962264
4	21.571511589814296	27.409661549505614	20.77753025999812	30.24129660068197
5	18.450323466489667	36.45619742423249	24.912446124526767	20.18103298475107
6	28.261656045874982	25.23364792146786	24.053116302654107	22.45157973000305
7	21.62216094416322	25.374244034172644	31.955626265877772	21.04796875578636
8	26.53218569910637	26.47032682792039	26.388513022695353	20.608974450277888
9	27.914574081090244	25.37865947282511	26.40587566694489	20.300890779139756
10	20.394184724861198	27.084927401642545	32.70152204443356	19.8193658290627
11	19.983135873017684	34.38142553149062	25.80476354613849	19.83067504935321
12	20.13751954899855	26.048937303619805	33.11271332978199	20.700829817599654
13	20.035067128910867	27.08028406938221	25.241268113980986	27.643380687725934
14	20.398286809802844	34.564011041445305	24.96977561025635	20.067926538495502
15	20.28463911622859	33.846844100831525	24.989160810275724	20.87935597266416
16	20.87810255782088	26.200344119347886	25.03062320355742	27.890930119273815
17	28.39295125070861	25.91663082090126	25.079250002136504	20.61116792625363
18	20.614671790474617	26.052312977686366	25.04463866044137	28.288376571397645
19	20.971652883851174	33.92323118951917	24.828638250232878	20.276477676396773
20	28.55488390245014	26.653966345811458	24.878319056748357	19.912830694990046
21	21.331582350779538	33.513863053033695	24.921433678687105	20.233120917499665
22	28.182334826986022	26.098461433279873	25.467751630151465	20.25145210958264
23	20.765038841616793	26.28993479394145	32.83258935565931	20.11243700878245
24	20.81853686606408	33.1384795508217	25.02964041237348	21.01334317074074
25	20.48176708703542	26.304135414381797	25.15889881808678	28.055198680496012
26	20.68207132500192	29.570876336382362	29.484234035340602	20.262818303275115
27	21.148113753094368	25.41714500585402	32.968670326258184	20.46607091479343
28	24.709421406616322	25.919337057494708	29.0722878524161	20.29895368347287
29	24.8488068345293	25.900706755051406	29.04247651983671	20.20800989058258
30	24.82606020379386	25.4572400374885	29.135599545352253	20.581100213365392
31	22.979808626342795	26.47729182631089	29.500770565261607	21.042128982084712
32	22.207306269068287	27.018453684897203	29.849590218806348	20.92464982722816
33	22.152483613025257	26.49902717916141	29.852239481997827	21.496249725815503
34	21.716152814058756	26.855082454755998	29.975586897257017	21.45317783392823
35	21.56028782962674	26.82675243062776	30.183482841035662	21.429476898709837
36	21.822308505274314	26.51688834067815	30.085417372899464	21.57538578114807
37	21.756917283166356	26.837719810506467	30.070333664728622	21.335029241598562
38	21.787953543888037	26.756276332394226	30.252477630818053	21.20329249289969
39	21.91745408655969	26.438649616426574	30.203950535692414	21.439945761321326
40	21.667810882489512	26.97513965605157	29.922943473839236	21.43410598761968
41	21.737332676240097	26.95098293361744	30.018388165484943	21.293296224657517
42	21.9876310744329	26.587934172931366	29.9620272275888	21.46240752504693
43	21.839343552462534	26.981164593309614	30.04124874302432	21.138243111203533
44	21.87674659085406	26.811967832817253	29.991083662592118	21.32020191373657
45	21.954472554487936	26.379696388740914	30.04421135992662	21.621619696844526
46	21.733159374545995	26.775675775764086	29.920621807709068	21.57054304198085
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	21673.0
1	12662.0
2	3651.0
3	2776.5
4	1902.0
5	1916.0
6	1930.0
7	1930.0
8	2280.0
9	2630.0
10	3653.0
11	4676.0
12	6830.5
13	8985.0
14	11685.5
15	14386.0
16	20180.0
17	25974.0
18	35962.5
19	45951.0
20	45951.0
21	59971.0
22	73991.0
23	94769.5
24	115548.0
25	269963.0
26	424378.0
27	312761.0
28	201144.0
29	212955.5
30	224767.0
31	224767.0
32	246309.5
33	267852.0
34	291675.0
35	315498.0
36	340821.5
37	366145.0
38	387824.0
39	409503.0
40	431152.0
41	452801.0
42	472714.0
43	492627.0
44	492627.0
45	506017.5
46	519408.0
47	521361.5
48	523315.0
49	514893.0
50	506471.0
51	486333.0
52	466195.0
53	437608.5
54	409022.0
55	378187.0
56	347352.0
57	347352.0
58	304624.0
59	261896.0
60	227441.0
61	192986.0
62	163017.5
63	133049.0
64	108425.5
65	83802.0
66	67709.5
67	51617.0
68	39297.0
69	26977.0
70	26977.0
71	19702.5
72	12428.0
73	9011.0
74	5594.0
75	4034.0
76	2474.0
77	1778.0
78	1082.0
79	812.0
80	542.0
81	542.0
82	444.5
83	347.0
84	245.0
85	143.0
86	100.0
87	57.0
88	41.5
89	26.0
90	16.5
91	7.0
92	7.0
93	7.0
94	7.0
95	7.0
96	7.0
97	5.0
98	3.0
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
46	7020820.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.91538172000434
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.00793125697665	35.94303375559822
2	15.559522158468948	15.533189758416869
3	5.1256862915420625	7.6755176345794665
4	2.3103312363287674	4.61284262244
5	1.2900717644030824	3.2197212283189685
6	0.8041968626363382	2.408507602591363
7	0.5613910650150403	1.961543451309786
8	0.40942704164626204	1.634936565621223
9	0.2782482040672814	1.2499978787031614
>10	1.5039123125291023	13.479550813045154
>50	0.09898148820295931	3.3874355079311025
>100	0.04888255334510615	4.0531434768825845
>500	0.0010633423554315775	0.32394486026847585
>1k	2.3628165535433208E-4	0.28106788391105725
>5k	2.953520690746341E-5	0.07383099723842448
>10k+	8.860562072239022E-5	4.161735963144282
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	249597	3.555097552707519	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	19233	0.2739423600092297	No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	13463	0.1917582276714116	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.546010295093735E-5	0.0	0.0	0.0	0.0
2	0.0011109813383621855	0.0	0.0	0.0	0.0
3	0.0011109813383621855	0.0	0.0	0.0	0.0
4	0.0013388749462313518	0.0	0.0	0.0	0.0
5	0.0013388749462313518	0.0	0.0	0.0	0.0
6	0.0013388749462313518	0.0	0.0	0.0	0.0
7	0.0013388749462313518	0.0	0.0	0.0	0.0
8	0.0013388749462313518	0.0	0.0	1.4243350491822892E-5	0.0
9	0.0013531182967231748	0.0	0.0	1.4243350491822892E-5	0.0
10	0.0013958483481986434	0.0	0.0	2.8486700983645785E-5	0.0
11	0.0014385783996741122	0.0	0.0	2.8486700983645785E-5	0.0
12	0.001452821750165935	0.0	0.0	2.8486700983645785E-5	0.0
13	0.0014955518016414038	0.0	0.0	2.8486700983645785E-5	0.0
14	0.0016237419560678097	0.0	0.0	2.8486700983645785E-5	0.0
15	0.0017376887600023929	0.0	0.0	4.2730051475468676E-5	0.0
16	0.001851635563936976	0.0	0.0	4.2730051475468676E-5	0.0
17	0.0019086089659042676	0.0	0.0	5.697340196729157E-5	0.0
18	0.002108015872789788	0.0	0.0	8.546010295093735E-5	0.0
19	0.0021507459242652567	0.0	0.0	8.546010295093735E-5	0.0
20	0.002407126233118069	0.0	0.0	8.546010295093735E-5	0.0
21	0.0025922897895117665	0.0	0.0	9.970345344276025E-5	0.0
22	0.0027632099954136412	0.0	0.0	1.2819015442640602E-4	0.0
23	0.0030053469537746303	0.0	0.0	1.2819015442640602E-4	0.0
24	0.0032759706131192654	0.0	0.0	1.2819015442640602E-4	2.8486700983645785E-5
25	0.0036605410763984833	0.0	0.0	1.2819015442640602E-4	2.8486700983645785E-5
26	0.004002381488202233	0.0	0.0	1.2819015442640602E-4	2.8486700983645785E-5
27	0.004358465250497805	0.0	0.0	1.2819015442640602E-4	4.2730051475468676E-5
28	0.0047287923632852	0.0	0.0	1.4243350491822891E-4	5.697340196729157E-5
29	0.005227309630499001	0.0	0.0	1.4243350491822891E-4	5.697340196729157E-5
30	0.005497933289843637	0.0	0.0	1.4243350491822891E-4	5.697340196729157E-5
31	0.005854017052139209	0.0	0.0	1.4243350491822891E-4	5.697340196729157E-5
32	0.006381021020336656	0.0	0.0	1.4243350491822891E-4	7.121675245911446E-5
33	0.006879538287550457	0.0	0.0	1.4243350491822891E-4	7.121675245911446E-5
34	0.007392298905256081	0.0	0.0	1.4243350491822891E-4	7.121675245911446E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	57990	0.0	37.7065	1
TCAACGC	62650	0.0	35.450912	12
CAACGCA	62725	0.0	35.402153	13
GTGGTAT	62015	0.0	35.401115	6
AGTGGTA	62105	0.0	35.346592	5
AGCAGTG	62460	0.0	35.26417	2
GCAGTGG	62600	0.0	35.239616	3
GGTATCA	62660	0.0	35.23779	8
ACGCAGA	62935	0.0	35.22364	15
TGGTATC	62385	0.0	35.184742	7
AACGCAG	63225	0.0	35.13167	14
ATCAACG	63280	0.0	35.10746	11
CAGTGGT	62715	0.0	35.053818	4
GTATCAA	63005	0.0	35.028965	9
TATCAAC	63125	0.0	34.95287	10
CGCAGAG	63475	0.0	34.93659	16
AGAGTAC	62225	0.0	34.90237	19
GAGTACT	31775	0.0	34.79465	20
GTACTTT	31875	0.0	34.57255	22
CAGAGTA	63245	0.0	34.50075	18
>>END_MODULE