FastQCFastQC Report
Thu 26 May 2016
SRR805221_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805221_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11952688
Sequences flagged as poor quality0
Sequence length46
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT777870.6507908513967737No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT194890.16305118982441438No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT256300.034.0538441
TCAACGC325300.027.16261712
CAACGCA327000.026.99082613
AGTGGTA330100.026.8221745
GTGGTAT330850.026.749286
AACGCAG330300.026.74538214
ACGCAGA331150.026.7190115
GGTATCA330850.026.7009228
ATCAACG333350.026.53667311
AGCAGTG328900.026.530862
TGGTATC334550.026.4414867
GCAGTGG332700.026.336043
GTATCAA335750.026.3231569
CGCAGAG340450.026.05962816
TATCAAC339850.025.99382210
AGAGTAC340150.025.67102819
CAGTGGT346000.025.6416194
GAGTACT148900.024.95634820
CAGAGTA352000.024.937518
GCAGAGT360250.024.52741217
Produced by FastQC (version 0.11.5)