##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805221_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11952688
Sequences flagged as poor quality	0
Sequence length	46
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.76620882265144	37.0	35.0	37.0	35.0	37.0
2	35.7821720101788	37.0	35.0	37.0	35.0	37.0
3	37.64032441907628	39.0	37.0	39.0	35.0	39.0
4	37.793556646002976	39.0	37.0	39.0	35.0	39.0
5	37.83517038175848	39.0	37.0	39.0	35.0	39.0
6	37.793461939272575	39.0	37.0	39.0	35.0	39.0
7	37.779454044144714	39.0	37.0	39.0	35.0	39.0
8	38.84438437613364	40.0	39.0	40.0	36.0	40.0
9	38.86067251148863	40.0	39.0	40.0	36.0	40.0
10	38.82498932457703	40.0	39.0	40.0	36.0	40.0
11	38.84231505080698	40.0	39.0	40.0	36.0	40.0
12	38.84242088474157	40.0	39.0	40.0	36.0	40.0
13	38.87711977422986	40.0	39.0	40.0	36.0	40.0
14	38.72908445363922	40.0	39.0	40.0	36.0	40.0
15	38.698848409663164	40.0	39.0	40.0	36.0	40.0
16	38.758280480507814	40.0	39.0	40.0	36.0	40.0
17	38.75314197107797	40.0	39.0	40.0	36.0	40.0
18	38.74319441785814	40.0	39.0	40.0	36.0	40.0
19	38.65412683741096	40.0	39.0	40.0	36.0	40.0
20	38.566251708402326	40.0	39.0	40.0	36.0	40.0
21	38.516503233414944	40.0	39.0	40.0	36.0	40.0
22	38.42400537853912	40.0	38.0	40.0	35.0	40.0
23	38.24055434225339	40.0	38.0	40.0	35.0	40.0
24	38.2668872474543	40.0	38.0	40.0	35.0	40.0
25	38.29573155427465	40.0	38.0	40.0	35.0	40.0
26	38.2265466981151	40.0	38.0	40.0	35.0	40.0
27	38.17166088498252	40.0	38.0	40.0	35.0	40.0
28	38.09372410624288	40.0	38.0	40.0	34.0	40.0
29	37.97691180427365	40.0	38.0	40.0	34.0	40.0
30	37.969620473654125	40.0	38.0	40.0	34.0	40.0
31	37.88803715114123	40.0	38.0	40.0	34.0	40.0
32	37.8474490424246	40.0	38.0	40.0	34.0	40.0
33	37.778498024879426	40.0	38.0	40.0	34.0	40.0
34	37.70980979341216	40.0	38.0	40.0	33.0	40.0
35	37.6518381472017	40.0	37.0	40.0	33.0	40.0
36	37.58625507500907	40.0	37.0	40.0	33.0	40.0
37	37.5071628239606	40.0	37.0	40.0	33.0	40.0
38	37.322009492760124	40.0	37.0	40.0	33.0	40.0
39	37.35701007170939	39.0	37.0	40.0	33.0	40.0
40	37.24282186567574	39.0	37.0	40.0	33.0	40.0
41	37.07640465475214	39.0	36.0	40.0	32.0	40.0
42	36.93231238027798	39.0	36.0	40.0	32.0	40.0
43	36.80868696647984	39.0	36.0	40.0	31.0	40.0
44	36.76284405650009	39.0	36.0	40.0	31.0	40.0
45	36.64674004709234	39.0	36.0	40.0	31.0	40.0
46	36.88978956030643	39.0	36.0	40.0	31.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	4.0
12	4.0
13	19.0
14	47.0
15	108.0
16	335.0
17	647.0
18	1004.0
19	1571.0
20	2482.0
21	4197.0
22	6932.0
23	11340.0
24	17359.0
25	25471.0
26	36036.0
27	49440.0
28	66797.0
29	87832.0
30	114275.0
31	147137.0
32	189562.0
33	244689.0
34	322947.0
35	443976.0
36	649800.0
37	1092086.0
38	2543988.0
39	5892603.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	14.955305450957976	31.292684959232602	16.747462997444593	37.00454659236483
2	23.67467468405433	25.77586731955189	29.292264635369047	21.257193361024733
3	16.811482069974552	24.465049200648423	32.48363046036172	26.2398382690153
4	16.79787006905894	28.20424995616049	31.23541750608733	23.762462468693236
5	22.497408114392346	28.858604859425764	29.051239353022517	19.592747673159376
6	22.398501491881994	26.151740930575617	30.50690355173665	20.942854025805744
7	22.894490343929334	27.385563816272956	29.215244303206106	20.5047015365916
8	21.8471443410888	25.547977157941375	29.91758004559309	22.68729845537673
9	21.92601362973751	24.93193999542195	31.207833752541685	21.93421262229885
10	21.561953261057262	26.52221826588295	29.51736044645355	22.39846802660623
11	21.464393615896274	28.491064102066417	27.916331456154463	22.128210825882846
12	21.57554016301605	26.66229554389774	29.100759594829213	22.661404698256995
13	21.933401089361656	27.18607730746423	26.70380085215978	24.176720751014333
14	22.266012465145916	28.85800248446207	26.66172663420981	22.2142584161822
15	21.92891674240974	28.428818689151765	26.74785788769857	22.89440668073993
16	22.623664233518017	26.74618462391054	26.394506407261698	24.235644735309748
17	24.44757196038247	26.443407541466822	26.47705687624407	22.631963621906635
18	22.198621766083075	26.83747789618536	26.503929492679806	24.459970845051757
19	22.9732676030697	28.256288459968165	26.25135869019588	22.519085246766252
20	24.32155009818712	26.810429587051882	26.51616941728923	22.351850897471763
21	22.772735304393454	28.229106289731646	26.62511562252775	22.373042783347145
22	24.158515641000587	26.3460068563657	26.788568395661294	22.70690910697242
23	22.564832278730943	26.798248226675035	28.507754908351995	22.129164586242023
24	22.267719194209704	28.15192699750885	26.722758930878143	22.857594877403308
25	22.390955072197986	26.55275533001447	26.593833956010567	24.462455641776977
26	22.46056284577996	27.39813002732105	27.92782677837822	22.21348034852077
27	22.660400739984176	26.152309840263545	28.738037837179387	22.44925158257289
28	23.807289205574513	26.228610668997636	27.282055718345532	22.682044407082323
29	23.975050632962226	26.367340969663054	27.40329204610712	22.254316351267597
30	23.579482707153403	26.229480766167406	27.54400516436136	22.647031362317833
31	23.23195418469887	26.512044822051745	27.264729071820497	22.991271921428886
32	23.036625736403394	26.850646482197142	27.48398519228478	22.628742589114683
33	22.81968708628553	26.631892340869268	27.595140105723498	22.953280467121708
34	22.97504962900395	26.64085266845416	27.32720874166547	23.056888960876414
35	22.998743044242435	26.75179842391937	27.473016948154257	22.776441583683937
36	22.89495885778998	26.558912940754414	27.562519828175887	22.98360837327972
37	23.020696265141364	26.62633710509301	27.250899546612445	23.10206708315318
38	23.108375287634043	26.73941627188796	27.50157119469696	22.650637245781034
39	22.893804305776243	26.55974120632949	27.598310940601813	22.948143547292457
40	22.983081295186487	26.69086652307832	27.275839543373003	23.050212638362183
41	23.02634353042596	26.740754882918388	27.50882479321806	22.724076793437593
42	22.88839129742197	26.522360493304937	27.658966752917834	22.930281456355257
43	22.930783435491662	26.62199498556308	27.337097730652722	23.110123848292535
44	23.059817172505465	26.67866006374466	27.45179996332206	22.80972280042782
45	22.937677282298342	26.460399535234252	27.616005705160212	22.985917477307197
46	22.963386980401395	26.535754969928107	27.301708201535924	23.199149848134578
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4627.0
1	3217.0
2	1807.0
3	1493.5
4	1180.0
5	1360.0
6	1540.0
7	1540.0
8	2225.5
9	2911.0
10	4183.0
11	5455.0
12	8662.5
13	11870.0
14	16476.0
15	21082.0
16	29637.5
17	38193.0
18	53444.0
19	68695.0
20	68695.0
21	89480.0
22	110265.0
23	139757.0
24	169249.0
25	246853.0
26	324457.0
27	307769.5
28	291082.0
29	320987.5
30	350893.0
31	350893.0
32	389575.0
33	428257.0
34	471758.5
35	515260.0
36	563556.5
37	611853.0
38	656290.0
39	700727.0
40	744605.5
41	788484.0
42	828047.0
43	867610.0
44	867610.0
45	893103.5
46	918597.0
47	930567.0
48	942537.0
49	932609.5
50	922682.0
51	893269.0
52	863856.0
53	815440.0
54	767024.0
55	708920.0
56	650816.0
57	650816.0
58	585148.5
59	519481.0
60	454465.5
61	389450.0
62	331409.5
63	273369.0
64	225226.0
65	177083.0
66	142199.5
67	107316.0
68	81825.0
69	56334.0
70	56334.0
71	41386.5
72	26439.0
73	19187.0
74	11935.0
75	8769.5
76	5604.0
77	4048.0
78	2492.0
79	1849.5
80	1207.0
81	1207.0
82	891.5
83	576.0
84	389.5
85	203.0
86	169.5
87	136.0
88	83.0
89	30.0
90	20.5
91	11.0
92	8.0
93	5.0
94	5.0
95	5.0
96	5.0
97	4.0
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
46	1.1952688E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.25286822399606
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.61252497643868	35.987347808285996
2	15.416872895779191	15.494841641153767
3	5.12795866116758	7.730838925732611
4	2.3369895211766476	4.697617057941989
5	1.3088216733777815	3.288602154048184
6	0.7931223305264582	2.3914003176872853
7	0.5653945753058025	1.9888889361183297
8	0.4218650998594963	1.6959945017233764
9	0.3317700242538543	1.5005155778550827
>10	1.946232134973188	18.152645755481316
>50	0.10891638376428242	3.6683450014750187
>100	0.029077240681072623	2.293812038515523
>500	3.150572100629704E-4	0.09710957129195917
>1k	1.0456911756139647E-4	0.16008170261353674
>5k	0.0	0.0
>10k+	3.485636842356307E-5	0.8519590100758763
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	77787	0.6507908513967737	No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	19489	0.16305118982441438	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.5935588714438124E-4	0.0	0.0	0.0	8.36631894014133E-6
2	2.760885250246639E-4	0.0	0.0	0.0	8.36631894014133E-6
3	2.760885250246639E-4	0.0	0.0	8.36631894014133E-6	8.36631894014133E-6
4	2.844548439648052E-4	0.0	0.0	8.36631894014133E-6	8.36631894014133E-6
5	0.001062522505397949	0.0	0.0	8.36631894014133E-6	8.36631894014133E-6
6	0.001112720419038797	0.0	0.0	8.36631894014133E-6	2.509895682042399E-5
7	0.001112720419038797	0.0	0.0	8.36631894014133E-6	2.509895682042399E-5
8	0.001112720419038797	8.36631894014133E-6	0.0	8.36631894014133E-6	2.509895682042399E-5
9	0.001112720419038797	8.36631894014133E-6	0.0	8.36631894014133E-6	3.346527576056532E-5
10	0.001112720419038797	8.36631894014133E-6	0.0	8.36631894014133E-6	3.346527576056532E-5
11	0.001137819375859221	8.36631894014133E-6	0.0	8.36631894014133E-6	4.1831594700706655E-5
12	0.0011796509705599275	8.36631894014133E-6	0.0	8.36631894014133E-6	5.8564232580989316E-5
13	0.0012549478410211995	8.36631894014133E-6	0.0	1.673263788028266E-5	6.693055152113064E-5
14	0.001263314159961341	8.36631894014133E-6	0.0	1.673263788028266E-5	6.693055152113064E-5
15	0.0012716804789014823	8.36631894014133E-6	0.0	1.673263788028266E-5	9.202950834155464E-5
16	0.0012800467978416237	8.36631894014133E-6	0.0	3.346527576056532E-5	1.0039582728169596E-4
17	0.0012800467978416237	8.36631894014133E-6	0.0	3.346527576056532E-5	1.0876214622183729E-4
18	0.0012800467978416237	8.36631894014133E-6	0.0	4.1831594700706655E-5	1.0876214622183729E-4
19	0.001313512073602189	8.36631894014133E-6	0.0	4.1831594700706655E-5	1.1712846516197863E-4
20	0.0013302447114824716	8.36631894014133E-6	0.0	5.019791364084798E-5	1.1712846516197863E-4
21	0.0013553436683028956	8.36631894014133E-6	0.0	5.019791364084798E-5	1.2549478410211996E-4
22	0.0013720763061831783	1.673263788028266E-5	0.0	5.019791364084798E-5	1.2549478410211996E-4
23	0.001388808944063461	1.673263788028266E-5	0.0	5.019791364084798E-5	1.3386110304226129E-4
24	0.001413907900883885	2.509895682042399E-5	0.0	5.019791364084798E-5	1.422274219824026E-4
25	0.0014473731766444502	2.509895682042399E-5	0.0	5.8564232580989316E-5	1.422274219824026E-4
26	0.0014724721334648742	2.509895682042399E-5	0.0	5.8564232580989316E-5	1.422274219824026E-4
27	0.0015226700471057222	2.509895682042399E-5	0.0	6.693055152113064E-5	1.5059374092254394E-4
28	0.0015477690039261461	2.509895682042399E-5	0.0	6.693055152113064E-5	1.5059374092254394E-4
29	0.0016146995554472768	2.509895682042399E-5	8.36631894014133E-6	6.693055152113064E-5	1.5059374092254394E-4
30	0.0016565311501479834	2.509895682042399E-5	8.36631894014133E-6	7.529687046127197E-5	1.5059374092254394E-4
31	0.001723461701669114	2.509895682042399E-5	8.36631894014133E-6	7.529687046127197E-5	1.5059374092254394E-4
32	0.0017987585721303862	2.509895682042399E-5	8.36631894014133E-6	7.529687046127197E-5	1.7569269774296795E-4
33	0.0019075207183522234	2.509895682042399E-5	8.36631894014133E-6	8.366318940141331E-5	2.2589061138381594E-4
34	0.0019660849509332127	2.509895682042399E-5	8.36631894014133E-6	8.366318940141331E-5	2.2589061138381594E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	25630	0.0	34.053844	1
TCAACGC	32530	0.0	27.162617	12
CAACGCA	32700	0.0	26.990826	13
AGTGGTA	33010	0.0	26.822174	5
GTGGTAT	33085	0.0	26.74928	6
AACGCAG	33030	0.0	26.745382	14
ACGCAGA	33115	0.0	26.71901	15
GGTATCA	33085	0.0	26.700922	8
ATCAACG	33335	0.0	26.536673	11
AGCAGTG	32890	0.0	26.53086	2
TGGTATC	33455	0.0	26.441486	7
GCAGTGG	33270	0.0	26.33604	3
GTATCAA	33575	0.0	26.323156	9
CGCAGAG	34045	0.0	26.059628	16
TATCAAC	33985	0.0	25.993822	10
AGAGTAC	34015	0.0	25.671028	19
CAGTGGT	34600	0.0	25.641619	4
GAGTACT	14890	0.0	24.956348	20
CAGAGTA	35200	0.0	24.9375	18
GCAGAGT	36025	0.0	24.527412	17
>>END_MODULE