FastQCFastQC Report
Thu 26 May 2016
SRR805220_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805220_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8195096
Sequences flagged as poor quality0
Sequence length46
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT1819602.2203522692107573No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGC110010.13423881794673304No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCC100260.1223414588431911No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGG83430.10180478666753873No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT675700.038.087911
TCAACGC712600.036.46926512
CAACGCA712550.036.46340613
AGCAGTG707650.036.4021762
GTGGTAT709150.036.2800526
AACGCAG716800.036.27232414
AGTGGTA708800.036.269755
ATCAACG716850.036.25026311
ACGCAGA716400.036.22836715
GTATCAA713450.036.1567049
GGTATCA713700.036.1524438
GCAGTGG716600.036.0480043
TGGTATC713950.035.9913147
TATCAAC717700.035.96767810
CGCAGAG721500.035.96673616
AGAGTAC709150.035.8485519
CAGTGGT723350.035.5733764
CAGAGTA721000.035.40360618
GCAGAGT729850.035.16338717
GTACATG491900.034.95019522
Produced by FastQC (version 0.11.5)