##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805220_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8195096
Sequences flagged as poor quality	0
Sequence length	46
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.02722518442737	37.0	35.0	37.0	32.0	37.0
2	35.17176833559973	37.0	35.0	37.0	32.0	37.0
3	37.041554973852655	39.0	37.0	39.0	33.0	39.0
4	37.18576524301851	39.0	37.0	39.0	33.0	39.0
5	37.29932876930301	39.0	37.0	39.0	34.0	39.0
6	37.35146922012872	39.0	37.0	39.0	34.0	39.0
7	37.10386992415952	39.0	37.0	39.0	33.0	39.0
8	38.1365625247099	40.0	38.0	40.0	34.0	40.0
9	38.22438821949127	40.0	38.0	40.0	34.0	40.0
10	37.97150063891869	40.0	38.0	40.0	34.0	40.0
11	38.15306580911316	40.0	38.0	40.0	34.0	40.0
12	38.20765162482538	40.0	38.0	40.0	34.0	40.0
13	38.27326940404359	40.0	38.0	40.0	34.0	40.0
14	38.17967708981078	40.0	38.0	40.0	34.0	40.0
15	38.174651767349644	40.0	38.0	40.0	34.0	40.0
16	38.18532009875174	40.0	38.0	40.0	34.0	40.0
17	38.22981024261339	40.0	38.0	40.0	34.0	40.0
18	38.178914560610394	40.0	38.0	40.0	34.0	40.0
19	38.071737292644286	40.0	38.0	40.0	34.0	40.0
20	37.975020182802005	40.0	38.0	40.0	34.0	40.0
21	37.79405256509503	40.0	38.0	40.0	33.0	40.0
22	37.78740541904573	40.0	38.0	40.0	33.0	40.0
23	37.360563317379075	40.0	37.0	40.0	32.0	40.0
24	37.458496032261245	40.0	37.0	40.0	32.0	40.0
25	37.55834464904377	40.0	37.0	40.0	33.0	40.0
26	37.500779246515236	40.0	37.0	40.0	33.0	40.0
27	37.425032848913546	40.0	37.0	40.0	32.0	40.0
28	37.34244125999256	40.0	37.0	40.0	32.0	40.0
29	37.177468451864385	40.0	37.0	40.0	31.0	40.0
30	37.20100838355036	40.0	37.0	40.0	31.0	40.0
31	37.07082931060234	40.0	37.0	40.0	31.0	40.0
32	36.99686763889038	40.0	37.0	40.0	31.0	40.0
33	36.91692031429528	39.0	36.0	40.0	31.0	40.0
34	36.77431210079784	39.0	36.0	40.0	30.0	40.0
35	36.777053984480474	39.0	36.0	40.0	30.0	40.0
36	36.675645410377136	39.0	36.0	40.0	30.0	40.0
37	36.56577946127782	39.0	36.0	40.0	30.0	40.0
38	36.41160774687691	39.0	35.0	40.0	30.0	40.0
39	36.35407748731681	38.0	35.0	40.0	30.0	40.0
40	36.2738510933856	38.0	35.0	40.0	30.0	40.0
41	35.92047658746157	38.0	35.0	40.0	29.0	40.0
42	35.851791730078574	38.0	35.0	40.0	29.0	40.0
43	35.73676928250749	38.0	35.0	40.0	29.0	40.0
44	35.60818811640522	38.0	34.0	40.0	28.0	40.0
45	35.4584283088325	38.0	34.0	40.0	28.0	40.0
46	35.68627383986716	38.0	34.0	40.0	28.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	16.0
11	41.0
12	73.0
13	153.0
14	268.0
15	509.0
16	886.0
17	1359.0
18	2263.0
19	3630.0
20	5742.0
21	9021.0
22	13742.0
23	20381.0
24	28759.0
25	39843.0
26	53114.0
27	70122.0
28	88830.0
29	110686.0
30	137147.0
31	166976.0
32	203541.0
33	247605.0
34	306779.0
35	393804.0
36	539462.0
37	851394.0
38	1734263.0
39	3164683.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	16.15661366260993	34.96937680778846	26.732951023392527	22.14105850620908
2	26.506962212523195	36.32498020767542	18.71582712392875	18.452230455872634
3	31.735235316340404	24.891130012387897	21.157275033751894	22.216359637519805
4	21.9195845905893	24.500481751525545	22.089198222937227	31.490735434947926
5	22.38439915774019	32.33077440459514	23.563836225957573	21.7209902117071
6	30.552393773056473	24.00579566121007	22.960060504477312	22.481750061256147
7	22.8077254982736	23.74402935609296	31.601289356463912	21.846955789169524
8	29.616175332174265	24.738489945694354	23.936412703402134	21.708922018729247
9	29.52630451186905	24.26004527585766	24.431367246948664	21.78228296532463
10	21.54444560503013	24.787153195032737	31.84384905314105	21.824552146796083
11	21.54525096472329	32.50794133467137	24.243266948916766	21.70354075168857
12	21.44968161446797	24.542799742675374	31.95541577548329	22.052102867373367
13	21.44892506445318	24.728300925333883	24.081145602199168	29.74162840801377
14	21.836327481703695	32.61908097232784	23.90701707460169	21.637574471366776
15	21.667690042923233	32.23079998086661	23.93007964763317	22.171430328576992
16	21.91475243243032	24.332710684536217	24.044623760356192	29.707913122677272
17	29.886422318908775	24.14691664380747	24.008529003198987	21.958132034084773
18	21.791141921949418	24.420848761259172	23.9873211979457	29.80068811884571
19	22.190002411198112	32.111204066431924	23.93188560573299	21.766907916636974
20	29.954877892827614	24.35483367125901	24.059193449350687	21.631094986562697
21	22.090345250379006	32.06736077283293	24.018742428398642	21.82355154838943
22	29.69329462400441	24.10601413333047	24.270832214778203	21.929859027886923
23	22.150881453981746	24.391184679227678	31.764606540301664	21.693327326488916
24	21.92254977855049	31.89340307910975	24.016875458200857	22.167171684138903
25	21.913288142079114	24.284962616667332	24.084855137755557	29.716894103497992
26	22.22104536664366	29.33972951628633	26.69187767904122	21.74734743802879
27	22.3700125050396	24.019315942119533	31.77995718415013	21.83071436869074
28	27.53822529961821	24.084098587740765	26.55782189738839	21.819854215252636
29	27.67602722408621	24.11102932778335	26.43460186433447	21.778341583795967
30	27.46228964248863	24.046368706358045	26.482899041085055	22.008442610068265
31	25.37467529361462	24.895705919735413	27.02839600658735	22.701222780062615
32	24.14439074295164	25.856841701427292	27.314054649268293	22.684712906352775
33	23.881453005553567	25.30281524462923	27.58825521995105	23.227476529866152
34	23.531756064846586	25.218301286525502	27.88011269178543	23.369829956842484
35	23.47491719438064	25.36933063383272	27.90114966316441	23.25460250862223
36	23.40788930355422	25.364730321646018	27.929032191935278	23.298348182864483
37	23.720991187900665	25.254310626745557	27.873755231177277	23.150942954176497
38	23.88551641127816	25.29492027915231	27.801455895086523	23.018107414483
39	23.750154970728836	25.248538882277888	27.827264012526516	23.17404213446676
40	23.542884671515747	25.456443707309834	27.6730986433838	23.32757297779062
41	23.742455243965416	25.656502376543237	27.545925026381628	23.055117353109715
42	23.688605966299843	25.554502351162206	27.650475357457683	23.106416325080268
43	23.645604639652788	25.405449795829117	27.88333413055808	23.06561143396002
44	23.830251652939758	25.319483749793783	27.66772957876271	23.18253501850375
45	23.775438384126314	25.024087576277328	27.736734261563257	23.46373977803311
46	23.673413953906092	25.182243136627076	27.56260573396578	23.581737175501054
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1355.0
1	877.5
2	400.0
3	354.5
4	309.0
5	391.0
6	473.0
7	473.0
8	675.0
9	877.0
10	1371.5
11	1866.0
12	2855.0
13	3844.0
14	5606.0
15	7368.0
16	10635.5
17	13903.0
18	19941.0
19	25979.0
20	25979.0
21	34428.0
22	42877.0
23	56476.5
24	70076.0
25	179473.5
26	288871.0
27	214563.5
28	140256.0
29	152126.0
30	163996.0
31	163996.0
32	186240.0
33	208484.0
34	235252.5
35	262021.0
36	294259.0
37	326497.0
38	359954.0
39	393411.0
40	430477.5
41	467544.0
42	508021.0
43	548498.0
44	548498.0
45	589930.0
46	631362.0
47	655166.5
48	678971.0
49	692201.0
50	705431.0
51	694639.5
52	683848.0
53	655887.0
54	627926.0
55	595409.5
56	562893.0
57	562893.0
58	499973.5
59	437054.0
60	381898.5
61	326743.0
62	278144.5
63	229546.0
64	189659.5
65	149773.0
66	121514.5
67	93256.0
68	72315.0
69	51374.0
70	51374.0
71	38266.0
72	25158.0
73	18549.5
74	11941.0
75	8987.5
76	6034.0
77	4322.0
78	2610.0
79	1926.5
80	1243.0
81	1243.0
82	915.5
83	588.0
84	414.0
85	240.0
86	194.0
87	148.0
88	94.0
89	40.0
90	22.0
91	4.0
92	3.5
93	3.0
94	3.0
95	3.0
96	3.0
97	2.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
46	8195096.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.04135093875124
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.19317852993971	44.03203186603968
2	12.133569058421692	13.842303416020105
3	4.020301073861613	6.879702133007361
4	1.9230295086134146	4.387688042655686
5	1.1546630416075603	3.2931769886171387
6	0.7615402085412021	2.606356937362111
7	0.5744560520627588	2.2937424485220967
8	0.4130061182383463	1.884674154422792
9	0.32728065351979696	1.680167755159793
>10	1.4525351852584156	13.593910824159968
>50	0.03392209112302616	1.2848563161005113
>100	0.011901622955802053	1.1652984810168583
>500	2.4237930386344805E-4	0.09432381428863953
>1k	2.8636442811636224E-4	0.3063870850464333
>5k	2.2028031119303748E-5	0.10483051696700625
>10k+	6.608409335791124E-5	2.5505492206138918
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	181960	2.2203522692107573	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGC	11001	0.13423881794673304	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCC	10026	0.1223414588431911	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGG	8343	0.10180478666753873	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.761935674701065E-5	0.0	0.0	0.0	1.2202419593376331E-5
2	1.8303629390064498E-4	0.0	0.0	0.0	3.6607258780128996E-5
3	1.8303629390064498E-4	0.0	0.0	0.0	6.1012097966881655E-5
4	1.8303629390064498E-4	0.0	0.0	0.0	6.1012097966881655E-5
5	1.8303629390064498E-4	0.0	0.0	0.0	9.761935674701065E-5
6	1.952387134940213E-4	0.0	0.0	0.0	1.3422661552713963E-4
7	2.0744113308739762E-4	0.0	0.0	0.0	1.3422661552713963E-4
8	2.1964355268077397E-4	0.0	0.0	0.0	1.8303629390064498E-4
9	2.1964355268077397E-4	0.0	0.0	0.0	1.8303629390064498E-4
10	2.318459722741503E-4	0.0	0.0	0.0	1.8303629390064498E-4
11	2.318459722741503E-4	0.0	0.0	0.0	1.8303629390064498E-4
12	2.4404839186752662E-4	0.0	0.0	0.0	1.952387134940213E-4
13	2.5625081146090294E-4	0.0	0.0	0.0	2.318459722741503E-4
14	2.6845323105427927E-4	0.0	0.0	0.0	2.4404839186752662E-4
15	2.6845323105427927E-4	0.0	0.0	0.0	2.5625081146090294E-4
16	2.8065565064765564E-4	0.0	0.0	0.0	2.5625081146090294E-4
17	2.9285807024103197E-4	0.0	0.0	0.0	2.6845323105427927E-4
18	3.050604898344083E-4	0.0	0.0	0.0	2.6845323105427927E-4
19	3.172629094277846E-4	0.0	0.0	0.0	2.8065565064765564E-4
20	3.5387016820791363E-4	0.0	0.0	0.0	3.050604898344083E-4
21	3.6607258780128996E-4	0.0	0.0	0.0	3.172629094277846E-4
22	4.026798465814189E-4	0.0	0.0	0.0	3.5387016820791363E-4
23	4.3928710536154795E-4	0.0	0.0	0.0	3.6607258780128996E-4
24	4.3928710536154795E-4	0.0	0.0	0.0	3.6607258780128996E-4
25	4.636919445483006E-4	0.0	0.0	0.0	3.6607258780128996E-4
26	5.613113012953113E-4	0.0	0.0	0.0	3.6607258780128996E-4
27	6.223233992621929E-4	0.0	0.0	0.0	3.904774269880426E-4
28	7.199427560092036E-4	0.0	0.0	0.0	4.026798465814189E-4
29	8.175621127562142E-4	0.0	0.0	0.0	4.026798465814189E-4
30	8.541693715363432E-4	0.0	0.0	0.0	4.1488226617479525E-4
31	0.0010494080850303644	0.0	0.0	0.0	4.270846857681716E-4
32	0.0011592298613707515	0.0	0.0	0.0	4.5148952495492427E-4
33	0.0013178613160846437	0.0	0.0	0.0	4.758943641416769E-4
34	0.0013910758336449019	0.0	0.0	0.0	4.8809678373505324E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	67570	0.0	38.08791	1
TCAACGC	71260	0.0	36.469265	12
CAACGCA	71255	0.0	36.463406	13
AGCAGTG	70765	0.0	36.402176	2
GTGGTAT	70915	0.0	36.280052	6
AACGCAG	71680	0.0	36.272324	14
AGTGGTA	70880	0.0	36.26975	5
ATCAACG	71685	0.0	36.250263	11
ACGCAGA	71640	0.0	36.228367	15
GTATCAA	71345	0.0	36.156704	9
GGTATCA	71370	0.0	36.152443	8
GCAGTGG	71660	0.0	36.048004	3
TGGTATC	71395	0.0	35.991314	7
TATCAAC	71770	0.0	35.967678	10
CGCAGAG	72150	0.0	35.966736	16
AGAGTAC	70915	0.0	35.84855	19
CAGTGGT	72335	0.0	35.573376	4
CAGAGTA	72100	0.0	35.403606	18
GCAGAGT	72985	0.0	35.163387	17
GTACATG	49190	0.0	34.950195	22
>>END_MODULE