FastQCFastQC Report
Thu 26 May 2016
SRR805219_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805219_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14685657
Sequences flagged as poor quality0
Sequence length46
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT1289790.8782650990691121No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT197750.13465519452074906No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT484250.035.78731
AGTGGTA561800.030.6585985
GTGGTAT564800.030.5134566
CAACGCA567250.030.44865613
TCAACGC567500.030.41762212
AACGCAG573850.030.11239614
GGTATCA572550.030.1109098
TGGTATC572500.030.096077
ACGCAGA575050.030.06695215
ATCAACG577650.029.93161811
GTATCAA577650.029.858919
AGCAGTG582200.029.7354852
TATCAAC582550.029.63179210
CAGTGGT582950.029.5908744
AGAGTAC581650.029.4300719
CGCAGAG588800.029.4055716
GCAGTGG590600.029.336273
CAGAGTA600350.028.62996718
GAGTACT205500.028.2043820
GCAGAGT613650.028.1887117
Produced by FastQC (version 0.11.5)