##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805219_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	14685657
Sequences flagged as poor quality	0
Sequence length	46
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.20174603015718	37.0	36.0	37.0	35.0	37.0
2	36.182475322690706	37.0	37.0	37.0	35.0	37.0
3	37.93664103689743	39.0	38.0	39.0	35.0	39.0
4	37.936493954611635	39.0	38.0	39.0	35.0	39.0
5	37.923372444283565	39.0	38.0	39.0	35.0	39.0
6	37.88065552668158	39.0	38.0	39.0	35.0	39.0
7	37.86104271671332	39.0	38.0	39.0	35.0	39.0
8	38.817945087509536	40.0	39.0	40.0	36.0	40.0
9	38.79217824575366	40.0	39.0	40.0	36.0	40.0
10	38.719810152177736	40.0	39.0	40.0	36.0	40.0
11	38.667287544574954	40.0	39.0	40.0	36.0	40.0
12	38.68086031152709	40.0	39.0	40.0	36.0	40.0
13	38.74241506525721	40.0	39.0	40.0	36.0	40.0
14	38.68141173391153	40.0	39.0	40.0	36.0	40.0
15	38.618912997899926	40.0	39.0	40.0	36.0	40.0
16	38.57928569351715	40.0	39.0	40.0	36.0	40.0
17	38.49582793606034	40.0	39.0	40.0	35.0	40.0
18	38.54357125459215	40.0	39.0	40.0	36.0	40.0
19	38.52617489295848	40.0	39.0	40.0	36.0	40.0
20	38.38444701520674	40.0	38.0	40.0	35.0	40.0
21	38.31764462427524	40.0	38.0	40.0	35.0	40.0
22	38.292917776848526	40.0	38.0	40.0	35.0	40.0
23	38.18301278587672	40.0	38.0	40.0	35.0	40.0
24	38.076462292425866	40.0	38.0	40.0	34.0	40.0
25	38.059227312744675	40.0	38.0	40.0	34.0	40.0
26	38.006729423137145	40.0	38.0	40.0	34.0	40.0
27	37.90461291585388	40.0	38.0	40.0	34.0	40.0
28	37.8144319998758	40.0	38.0	40.0	34.0	40.0
29	37.751277045351124	40.0	38.0	40.0	34.0	40.0
30	37.72029920077801	40.0	38.0	40.0	34.0	40.0
31	37.583577295860856	40.0	37.0	40.0	33.0	40.0
32	37.537564100809384	40.0	37.0	40.0	33.0	40.0
33	37.45749965425449	39.0	37.0	40.0	33.0	40.0
34	37.3344841160324	39.0	37.0	40.0	33.0	40.0
35	37.24449610936712	39.0	37.0	40.0	33.0	40.0
36	37.11885433521973	39.0	36.0	40.0	33.0	40.0
37	37.020468679065566	39.0	36.0	40.0	33.0	40.0
38	36.84955654350364	38.0	36.0	40.0	32.0	40.0
39	36.78698508347294	38.0	36.0	40.0	32.0	40.0
40	36.63848529214594	38.0	36.0	40.0	31.0	40.0
41	36.45168888256072	38.0	35.0	40.0	31.0	40.0
42	36.3030367657368	38.0	35.0	40.0	31.0	40.0
43	36.10585913861396	38.0	35.0	40.0	31.0	40.0
44	36.05088161871137	38.0	35.0	40.0	31.0	40.0
45	35.9854731048124	38.0	35.0	40.0	30.0	40.0
46	36.68807762567245	38.0	36.0	40.0	32.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	10.0
12	30.0
13	76.0
14	188.0
15	514.0
16	1063.0
17	1797.0
18	2631.0
19	3587.0
20	5393.0
21	8197.0
22	13076.0
23	19780.0
24	29047.0
25	40704.0
26	55862.0
27	73252.0
28	96352.0
29	122177.0
30	154890.0
31	193389.0
32	243557.0
33	308329.0
34	400724.0
35	546792.0
36	813760.0
37	1443384.0
38	4108534.0
39	5998561.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.26076293352078	39.368037807229186	15.841511210564157	19.52968804868587
2	30.09971566134222	30.03828837892646	25.43283558917385	14.429160370557476
3	25.02915599894509	24.510214285952614	26.20809542262903	24.252534292473264
4	21.53674159760098	30.19715086631807	24.104410173817897	24.161697362263055
5	22.32943340566922	30.588668930508184	23.64145506053968	23.440442603282918
6	24.579840043928574	24.98526283161863	28.296125941113836	22.138771183338953
7	21.991716135001653	26.094630972247273	28.00399736967846	23.909655523072615
8	24.22675403626818	25.108062921529488	27.024599580393303	23.64058346180903
9	25.08816595675631	25.59414263863033	26.682953306072722	22.634738098540637
10	22.18598732082603	27.0343029256369	28.851967603492305	21.927742150044768
11	22.088497640929514	29.374075671248484	26.642635055414953	21.89479163240705
12	22.13565930349592	25.661868583748078	29.172164377800737	23.03030773495527
13	22.364508445212905	26.537484839799813	25.818729117805216	25.279277597182066
14	22.473580855115983	29.215206374491792	25.917914329607456	22.393298440784772
15	22.527279508162284	28.46836883089398	25.718236507907	23.286115153036736
16	23.311480037971744	25.87007173053272	25.341147488328236	25.477300743167298
17	25.744765794271242	25.699592466309134	25.568192148298163	22.987449591121457
18	22.716484526364738	25.923641005642445	25.478124676342368	25.881749791650456
19	23.49979983871338	28.65455730036457	25.166936692039044	22.67870616888301
20	25.75543606935665	26.163820930857913	25.48464123872701	22.596101761058428
21	23.408581584058513	28.322532658906578	25.41752813646676	22.851357620568148
22	25.851706872903268	25.75715883872271	25.743124737286184	22.648009551087842
23	22.87476140835919	26.105845996539344	28.46651668359134	22.552875911510124
24	22.89252023249624	28.212568222177598	25.46599719712914	23.42891434819702
25	22.99412276890302	25.984578013772214	25.34789556912571	25.673403648199056
26	22.92816044934183	27.495855309707967	27.031041239762033	22.544943001188166
27	23.348604696405477	25.20226367809081	28.492569314399756	22.956562311103955
28	25.30648782005463	25.684176063760717	26.29234769680376	22.716988419380897
29	25.218211211115715	25.61653183102397	26.537743595672975	22.627513362187337
30	25.060315653565922	25.303226134179763	26.5588390087008	23.07761920355351
31	24.458789960844108	26.004270697592897	26.4099181943307	23.127021147232295
32	23.784812623636792	26.285116151085376	26.841202950606842	23.088868274670993
33	23.694023358982168	25.802904153351804	26.845717559657018	23.65735492800901
34	23.747170453456732	26.126171951312767	26.691240303379004	23.435417291851497
35	23.51917248237515	26.12305326210465	27.006718187684758	23.35105606783544
36	23.618275981796387	25.790599630646422	26.923623505574177	23.66750088198301
37	23.865973446063734	26.160981425618207	26.60314754729734	23.36989758102072
38	23.742832887898714	26.090913058911834	26.96110225099224	23.205151802197204
39	23.74353425250229	25.756797942373293	26.897278072067188	23.602389733057226
40	23.785037332684535	26.21726082803105	26.533126846146548	23.464574993137862
41	23.65359615848307	26.245921445666337	26.85136252331101	23.24911987253958
42	23.719967039949253	25.8799861660939	26.881453107613773	23.518593686343074
43	23.8961457427475	26.167062188637523	26.533406030114964	23.403386038500017
44	23.757847537907224	26.05018624634907	26.835169853143103	23.356796362600598
45	23.721349341061146	25.708403784726823	26.818861423768787	23.751385450443248
46	23.85139459542055	26.076776816999065	26.492304702472623	23.57952388510776
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	6826.0
1	4572.0
2	2318.0
3	2049.5
4	1781.0
5	1906.5
6	2032.0
7	2032.0
8	2644.5
9	3257.0
10	4517.5
11	5778.0
12	8931.0
13	12084.0
14	16251.0
15	20418.0
16	28198.5
17	35979.0
18	49315.0
19	62651.0
20	62651.0
21	81538.5
22	100426.0
23	126284.0
24	152142.0
25	256831.5
26	361521.0
27	320061.5
28	278602.0
29	310184.5
30	341767.0
31	341767.0
32	386843.5
33	431920.0
34	481964.0
35	532008.0
36	589937.0
37	647866.0
38	705382.0
39	762898.0
40	826069.0
41	889240.0
42	951956.0
43	1014672.0
44	1014672.0
45	1069125.5
46	1123579.0
47	1156249.5
48	1188920.0
49	1199109.0
50	1209298.0
51	1188821.5
52	1168345.0
53	1119197.0
54	1070049.0
55	1002536.5
56	935024.0
57	935024.0
58	842045.5
59	749067.0
60	656806.5
61	564546.0
62	484164.0
63	403782.0
64	335557.5
65	267333.0
66	216964.5
67	166596.0
68	128852.0
69	91108.0
70	91108.0
71	67410.0
72	43712.0
73	31993.5
74	20275.0
75	14924.0
76	9573.0
77	6926.0
78	4279.0
79	3185.0
80	2091.0
81	2091.0
82	1612.5
83	1134.0
84	835.5
85	537.0
86	361.0
87	185.0
88	106.0
89	27.0
90	17.0
91	7.0
92	4.5
93	2.0
94	2.0
95	1.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
46	1.4685657E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.878112509683625
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.75551633788852	34.83396796925551
2	13.973803057314141	13.380786299324948
3	4.910268292903479	7.052831333410947
4	2.379521581463662	4.5570800798615005
5	1.355208129066533	3.244240363874266
6	0.8411965616188976	2.4164942171969157
7	0.5987984794573136	2.0068538679059316
8	0.4432442820364251	1.6977359683691118
9	0.33052150362944943	1.4242271163876525
>10	2.227943391295542	20.302535511545695
>50	0.14762142265457498	4.689704629417828
>100	0.03508021875772589	2.764925975092656
>500	0.0010493012359133413	0.3354897844903827
>1k	1.421588159344619E-4	0.09894612350138746
>5k	5.685457462233925E-5	0.1818764246724388
>10k+	2.8427287311169624E-5	1.0123043356927324
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	128979	0.8782650990691121	No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	19775	0.13465519452074906	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.1789968266315902E-4	0.0	0.0	0.0	0.0
2	2.1789968266315902E-4	0.0	0.0	0.0	6.809365083223719E-6
3	2.1789968266315902E-4	0.0	0.0	0.0	6.809365083223719E-6
4	2.1789968266315902E-4	0.0	0.0	0.0	2.7237460332894877E-5
5	2.1789968266315902E-4	0.0	0.0	0.0	3.40468254161186E-5
6	2.2470904774638274E-4	0.0	0.0	0.0	3.40468254161186E-5
7	2.2470904774638274E-4	0.0	0.0	6.809365083223719E-6	3.40468254161186E-5
8	2.3151841282960647E-4	0.0	0.0	6.809365083223719E-6	5.4474920665789754E-5
9	2.3151841282960647E-4	0.0	0.0	6.809365083223719E-6	6.80936508322372E-5
10	2.3151841282960647E-4	6.809365083223719E-6	0.0	6.809365083223719E-6	8.171238099868463E-5
11	2.3151841282960647E-4	6.809365083223719E-6	0.0	6.809365083223719E-6	8.171238099868463E-5
12	2.3151841282960647E-4	6.809365083223719E-6	0.0	6.809365083223719E-6	1.021404762483558E-4
13	2.383277779128302E-4	6.809365083223719E-6	0.0	6.809365083223719E-6	1.021404762483558E-4
14	2.5875587316250134E-4	6.809365083223719E-6	0.0	6.809365083223719E-6	1.021404762483558E-4
15	2.6556523824572506E-4	6.809365083223719E-6	0.0	6.809365083223719E-6	1.0894984133157951E-4
16	2.6556523824572506E-4	6.809365083223719E-6	0.0	6.809365083223719E-6	1.2937793658125067E-4
17	2.8599333349539624E-4	6.809365083223719E-6	0.0	6.809365083223719E-6	1.4980603183092182E-4
18	2.9961206366184364E-4	6.809365083223719E-6	0.0	6.809365083223719E-6	1.4980603183092182E-4
19	3.132307938282911E-4	6.809365083223719E-6	0.0	6.809365083223719E-6	1.9066222233026414E-4
20	3.200401589115148E-4	6.809365083223719E-6	0.0	6.809365083223719E-6	2.3151841282960647E-4
21	3.40468254161186E-4	6.809365083223719E-6	0.0	6.809365083223719E-6	2.3151841282960647E-4
22	3.6770571449408083E-4	6.809365083223719E-6	0.0	6.809365083223719E-6	2.5875587316250134E-4
23	3.813244446605283E-4	1.3618730166447438E-5	0.0	6.809365083223719E-6	2.723746033289488E-4
24	4.153712700766469E-4	1.3618730166447438E-5	0.0	6.809365083223719E-6	2.791839684121725E-4
25	4.494180954927655E-4	1.3618730166447438E-5	0.0	6.809365083223719E-6	2.791839684121725E-4
26	4.766555558256604E-4	2.042809524967116E-5	0.0	6.809365083223719E-6	2.8599333349539624E-4
27	5.038930161585552E-4	2.042809524967116E-5	0.0	1.3618730166447438E-5	2.9280269857861997E-4
28	5.243211114082264E-4	2.042809524967116E-5	0.0	2.042809524967116E-5	3.132307938282911E-4
29	5.719866669907925E-4	2.042809524967116E-5	0.0	2.042809524967116E-5	3.200401589115148E-4
30	6.536990479894771E-4	2.042809524967116E-5	0.0	2.042809524967116E-5	3.2684952399473854E-4
31	7.558395242378328E-4	2.042809524967116E-5	0.0	2.042809524967116E-5	3.2684952399473854E-4
32	9.192642862352021E-4	2.042809524967116E-5	0.0	2.7237460332894877E-5	3.40468254161186E-4
33	0.0010894984133157952	2.7237460332894877E-5	0.0	2.7237460332894877E-5	3.472776192444097E-4
34	0.0011848295244809273	2.7237460332894877E-5	0.0	2.7237460332894877E-5	3.5408698432763344E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	48425	0.0	35.7873	1
AGTGGTA	56180	0.0	30.658598	5
GTGGTAT	56480	0.0	30.513456	6
CAACGCA	56725	0.0	30.448656	13
TCAACGC	56750	0.0	30.417622	12
AACGCAG	57385	0.0	30.112396	14
GGTATCA	57255	0.0	30.110909	8
TGGTATC	57250	0.0	30.09607	7
ACGCAGA	57505	0.0	30.066952	15
ATCAACG	57765	0.0	29.931618	11
GTATCAA	57765	0.0	29.85891	9
AGCAGTG	58220	0.0	29.735485	2
TATCAAC	58255	0.0	29.631792	10
CAGTGGT	58295	0.0	29.590874	4
AGAGTAC	58165	0.0	29.43007	19
CGCAGAG	58880	0.0	29.40557	16
GCAGTGG	59060	0.0	29.33627	3
CAGAGTA	60035	0.0	28.629967	18
GAGTACT	20550	0.0	28.20438	20
GCAGAGT	61365	0.0	28.18871	17
>>END_MODULE