FastQCFastQC Report
Thu 26 May 2016
SRR805218_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805218_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18899880
Sequences flagged as poor quality0
Sequence length46
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT8858294.686955684374715No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGC413560.21881620412404734No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGG303600.16063594054565425No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT265740.1406040673274116No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA206160.1090800576511597No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1932450.038.5665861
AGCAGTG2017750.036.9480862
TCAACGC2018550.036.9037212
GTGGTAT2010050.036.8687326
CAACGCA2022750.036.84093513
AGTGGTA2014700.036.7886055
ATCAACG2026250.036.7565711
AACGCAG2032250.036.688414
ACGCAGA2033950.036.66166715
GGTATCA2025400.036.6357278
GTATCAA2025850.036.63459
GCAGTGG2035100.036.5839543
TGGTATC2027050.036.5684137
TATCAAC2035150.036.5093510
GAGTACT1081600.036.45525420
CGCAGAG2050250.036.37507616
GTACTTT1074950.036.37006422
AGAGTAC2029500.036.32126219
CAGTGGT2046850.036.2234654
CAGAGTA2061800.035.90163818
Produced by FastQC (version 0.11.5)