##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805218_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	18899880
Sequences flagged as poor quality	0
Sequence length	46
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.275597834483605	37.0	37.0	37.0	35.0	37.0
2	36.21672767234501	37.0	37.0	37.0	35.0	37.0
3	37.97565603591134	39.0	38.0	39.0	35.0	39.0
4	37.94204000237038	39.0	38.0	39.0	35.0	39.0
5	37.9030500193652	39.0	38.0	39.0	35.0	39.0
6	37.88872072203633	39.0	38.0	39.0	35.0	39.0
7	37.78888617282226	39.0	38.0	39.0	35.0	39.0
8	38.7973300359579	40.0	39.0	40.0	36.0	40.0
9	38.81165679358811	40.0	39.0	40.0	36.0	40.0
10	38.545358118675885	40.0	38.0	40.0	36.0	40.0
11	38.65954937280025	40.0	39.0	40.0	36.0	40.0
12	38.735794089697926	40.0	39.0	40.0	36.0	40.0
13	38.823860998059246	40.0	39.0	40.0	36.0	40.0
14	38.7829097856706	40.0	39.0	40.0	36.0	40.0
15	38.713768394296686	40.0	39.0	40.0	36.0	40.0
16	38.68079442832441	40.0	39.0	40.0	36.0	40.0
17	38.562091452432504	40.0	39.0	40.0	36.0	40.0
18	38.62043050008783	40.0	39.0	40.0	36.0	40.0
19	38.59507245548649	40.0	39.0	40.0	36.0	40.0
20	38.46935583717992	40.0	38.0	40.0	35.0	40.0
21	38.32179251931758	40.0	38.0	40.0	35.0	40.0
22	38.38493694139857	40.0	38.0	40.0	35.0	40.0
23	38.08478926850329	40.0	38.0	40.0	34.0	40.0
24	38.08339529139867	40.0	38.0	40.0	34.0	40.0
25	38.10521257277824	40.0	38.0	40.0	34.0	40.0
26	38.09367345189493	40.0	38.0	40.0	34.0	40.0
27	38.01891863863686	40.0	38.0	40.0	34.0	40.0
28	37.920575157090944	40.0	38.0	40.0	34.0	40.0
29	37.83615213429927	40.0	38.0	40.0	34.0	40.0
30	37.8079999449732	40.0	38.0	40.0	34.0	40.0
31	37.66029768442974	40.0	37.0	40.0	33.0	40.0
32	37.627888695589604	40.0	37.0	40.0	33.0	40.0
33	37.49779697013949	40.0	37.0	40.0	33.0	40.0
34	37.386544411922195	39.0	37.0	40.0	33.0	40.0
35	37.30608559419425	39.0	37.0	40.0	33.0	40.0
36	37.14242302067527	39.0	36.0	40.0	33.0	40.0
37	36.96952948907612	38.0	36.0	40.0	33.0	40.0
38	36.72938341407459	38.0	35.0	40.0	32.0	40.0
39	36.66135859063655	38.0	35.0	40.0	32.0	40.0
40	36.519293138369136	38.0	35.0	40.0	31.0	40.0
41	36.310339271995375	38.0	35.0	40.0	31.0	40.0
42	36.15954963735219	38.0	35.0	40.0	31.0	40.0
43	35.98480429505373	38.0	35.0	40.0	31.0	40.0
44	35.915447611307584	38.0	35.0	40.0	31.0	40.0
45	35.835953191237195	38.0	35.0	39.0	30.0	40.0
46	36.40470415685179	38.0	35.0	40.0	31.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	4.0
11	8.0
12	14.0
13	55.0
14	174.0
15	532.0
16	1081.0
17	1832.0
18	2584.0
19	3593.0
20	5575.0
21	9086.0
22	14964.0
23	23559.0
24	35022.0
25	50576.0
26	69625.0
27	93692.0
28	121649.0
29	155653.0
30	197188.0
31	246183.0
32	309900.0
33	392498.0
34	512908.0
35	705363.0
36	1059784.0
37	1950732.0
38	5452262.0
39	7483781.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	12.859547256384696	39.76545882831002	23.738198337767223	23.63679557753806
2	27.356110197525062	43.69907110521336	12.878827802081283	16.06599089518029
3	34.750247091515924	26.07459412440714	18.43966734180323	20.735491442273705
4	21.489845438172097	24.092766726561226	20.02701075350743	34.39037708175925
5	20.09734982444333	34.24386821503629	23.625880164318502	22.03290179620188
6	31.22249453435683	23.519704887015155	22.33723177078373	22.920568807844283
7	22.451137255897923	23.045723041627777	32.2363422413264	22.266797461147902
8	30.413198390677614	24.713506117499158	23.745388859611808	21.12790663221142
9	30.89592103230285	24.08386719915682	23.837902674514336	21.182309094025996
10	21.058768627102395	24.799242111590125	32.982537455264264	21.159451806043215
11	20.98871527226628	34.47396491406295	23.611514993745992	20.92580481992478
12	20.750348679462515	24.49789628293936	33.21615798618827	21.535597051409848
13	20.868233025818153	24.800607199622434	23.43516995875106	30.895989815808356
14	21.421797387073358	34.51404453361609	23.18845410658692	20.875703972723635
15	21.141800900323176	34.037464788136226	23.19622135166996	21.624512959870646
16	21.655645432669413	24.088036537798125	23.316327934357258	30.939990095175208
17	31.272045113513947	24.104285318213662	23.290105545643677	21.333564022628714
18	21.288251565618406	24.310027365253113	23.303645314150142	31.09807575497834
19	21.693386413035427	33.80789719299805	23.27687794843142	21.221838445535106
20	31.307696133520423	24.358408624816665	23.411185679485797	20.922709562177115
21	21.620766904340133	33.73265332901585	23.426217520957803	21.220362245686218
22	30.899677669911128	23.946601777365785	23.835796841038146	21.31792371168494
23	21.36433670478331	24.343366201266885	33.255607972114106	21.036689121835693
24	21.344140809359637	33.48845601136092	23.518308052749543	21.649095126529904
25	21.235542236247003	24.110280065270256	23.630271726592973	31.02390597188977
26	21.50227408851273	28.529847808557513	28.92350639263318	21.044371710296573
27	21.749307403009965	23.84616198621367	33.178607483222116	21.22592312755425
28	26.03811241129573	23.92201431966764	28.747235432182638	21.292637836853988
29	26.11888541091266	24.092010108000686	28.636361712349494	21.152742768737156
30	26.038239396228967	23.881495543887052	28.707399200418205	21.372865859465776
31	24.677997955542573	24.53974310948006	28.98168136517269	21.800577569804677
32	23.489233794077	25.217482862325053	29.238698870045738	22.054584473552215
33	23.202967426248208	24.680728131607186	29.583134919375148	22.533169522769455
34	22.776626094980497	24.788903421609028	29.701479586113777	22.732990897296705
35	22.73127130965911	24.858221322040137	30.01774085338108	22.39276651491967
36	22.71889556970732	24.775014444536154	30.07126500274076	22.434824983015766
37	23.08660689909142	24.715432055653263	29.817337464576493	22.38062358067882
38	23.14868665832799	24.930877868007627	29.827369274302274	22.093066199362113
39	23.013225480796702	24.780030349399045	29.93704192830854	22.26970224149571
40	22.809647468661176	25.087915902111547	29.71552729435319	22.386909334874083
41	23.082940209144184	25.233546456379617	29.497123791262165	22.18638954321403
42	22.941558359100693	25.142593497948134	29.67503497376703	22.240813169184143
43	22.836742878790766	25.036481713111407	29.888512519656206	22.23826288844162
44	23.073273481101467	24.99796295002931	29.660071915800522	22.268691653068696
45	23.145432669413772	24.650198837241295	29.647844324937513	22.556524168407417
46	22.896007805340563	24.76244822718451	29.53528276370009	22.806261203774838
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	21518.0
1	12669.5
2	3821.0
3	3237.5
4	2654.0
5	2789.5
6	2925.0
7	2925.0
8	3615.5
9	4306.0
10	6002.0
11	7698.0
12	11114.5
13	14531.0
14	19049.5
15	23568.0
16	31142.0
17	38716.0
18	53441.5
19	68167.0
20	68167.0
21	87484.5
22	106802.0
23	140536.5
24	174271.0
25	665976.0
26	1157681.0
27	749310.5
28	340940.0
29	355119.5
30	369299.0
31	369299.0
32	417527.5
33	465756.0
34	524695.0
35	583634.0
36	662245.0
37	740856.0
38	821622.5
39	902389.0
40	988482.0
41	1074575.0
42	1157444.5
43	1240314.0
44	1240314.0
45	1321338.0
46	1402362.0
47	1454593.0
48	1506824.0
49	1531790.5
50	1556757.0
51	1529087.0
52	1501417.0
53	1446536.0
54	1391655.0
55	1332686.0
56	1273717.0
57	1273717.0
58	1112577.0
59	951437.0
60	830975.0
61	710513.0
62	605227.0
63	499941.0
64	410859.0
65	321777.0
66	260439.5
67	199102.0
68	157690.0
69	116278.0
70	116278.0
71	88894.5
72	61511.0
73	46250.0
74	30989.0
75	23315.0
76	15641.0
77	11541.5
78	7442.0
79	5876.5
80	4311.0
81	4311.0
82	3388.0
83	2465.0
84	1609.0
85	753.0
86	622.5
87	492.0
88	270.0
89	48.0
90	32.0
91	16.0
92	10.5
93	5.0
94	5.0
95	4.0
96	3.0
97	3.0
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
46	1.889988E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.7933143969869
#Duplication Level	Percentage of deduplicated	Percentage of total
1	48.23052493783129	12.922556181910307
2	19.673265463244377	10.542239735441814
3	10.335998922680648	8.308070062269016
4	6.015301148187213	6.44679419423745
5	3.800185272348913	5.090977938442186
6	2.5008074314935147	4.020295185499621
7	1.676056765625268	3.1434981108016355
8	1.2488290174227823	2.676821479351107
9	0.8769868937923191	2.114764701067311
>10	5.077232760462304	25.74117048669683
>50	0.44922385905712836	8.135321565028107
>100	0.1125136354974805	4.50835202728036
>500	0.001734780049887792	0.3131186272063894
>1k	0.001122608624570763	0.4946827415241476
>5k	9.841076465891648E-5	0.18473601756336494
>10k+	1.1809291759069976E-4	5.356600945680352
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	885829	4.686955684374715	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGC	41356	0.21881620412404734	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGG	30360	0.16063594054565425	No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	26574	0.1406040673274116	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	20616	0.1090800576511597	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.1164155539611892E-4	0.0	5.2910388849029725E-6	5.2910388849029725E-6	1.0582077769805945E-5
2	3.068802553243724E-4	0.0	5.2910388849029725E-6	5.2910388849029725E-6	1.0582077769805945E-5
3	3.703727219432081E-4	0.0	5.2910388849029725E-6	1.587311665470892E-5	3.703727219432081E-5
4	5.026486940657825E-4	0.0	5.2910388849029725E-6	1.587311665470892E-5	4.232831107922378E-5
5	5.555590829148121E-4	0.0	5.2910388849029725E-6	1.587311665470892E-5	6.878350550373865E-5
6	6.190515495336479E-4	0.0	5.2910388849029725E-6	1.587311665470892E-5	6.878350550373865E-5
7	6.613798606128716E-4	0.0	5.2910388849029725E-6	5.291038884902973E-5	6.878350550373865E-5
8	7.354544050015132E-4	0.0	5.2910388849029725E-6	5.291038884902973E-5	7.407454438864162E-5
9	7.460364827713192E-4	0.0	5.2910388849029725E-6	7.407454438864162E-5	8.465662215844756E-5
10	7.460364827713192E-4	0.0	5.2910388849029725E-6	7.93655832735446E-5	8.994766104335054E-5
11	7.460364827713192E-4	0.0	5.2910388849029725E-6	9.523869992825351E-5	1.1640285546786541E-4
12	7.77782716080737E-4	0.0	5.2910388849029725E-6	1.0582077769805946E-4	1.2169389435276837E-4
13	8.306931049297668E-4	0.0	5.2910388849029725E-6	1.0582077769805946E-4	1.2698493323767135E-4
14	8.571482993542817E-4	0.0	5.2910388849029725E-6	1.2698493323767135E-4	1.6402220543199216E-4
15	8.888945326636995E-4	0.0	5.2910388849029725E-6	1.4285804989238026E-4	1.6402220543199216E-4
16	9.418049215127292E-4	0.0	5.2910388849029725E-6	1.534401276621862E-4	1.6931324431689512E-4
17	0.0010529167380956916	0.0	5.2910388849029725E-6	1.534401276621862E-4	2.0105947762631297E-4
18	0.0011375733602541393	0.0	5.2910388849029725E-6	1.534401276621862E-4	2.2751467205082783E-4
19	0.0011957747879880719	0.0	5.2910388849029725E-6	1.534401276621862E-4	2.4338778870553675E-4
20	0.0013068866045710342	0.0	5.2910388849029725E-6	1.6402220543199216E-4	2.857160997847605E-4
21	0.0014179984211539968	5.2910388849029725E-6	5.2910388849029725E-6	1.6402220543199216E-4	2.962981775545665E-4
22	0.0014497446544634147	5.2910388849029725E-6	5.2910388849029725E-6	1.746042832017981E-4	3.333354497488873E-4
23	0.001534401276621862	5.2910388849029725E-6	5.2910388849029725E-6	1.7989532208670107E-4	3.4391752751869323E-4
24	0.001597893743240698	1.587311665470892E-5	5.2910388849029725E-6	1.7989532208670107E-4	3.544996052884992E-4
25	0.0016772593265142425	1.587311665470892E-5	5.2910388849029725E-6	1.7989532208670107E-4	3.544996052884992E-4
26	0.001735460754248175	1.587311665470892E-5	5.2910388849029725E-6	1.9576843874141E-4	3.5979064417340214E-4
27	0.001931229192989585	1.587311665470892E-5	5.2910388849029725E-6	1.9576843874141E-4	3.8095479971301404E-4
28	0.0021322886706158983	1.587311665470892E-5	5.2910388849029725E-6	1.9576843874141E-4	4.0211895525262594E-4
29	0.0022010721761196368	1.587311665470892E-5	5.2910388849029725E-6	2.1164155539611892E-4	4.0740999413752893E-4
30	0.002354512303781823	1.587311665470892E-5	5.2910388849029725E-6	2.2751467205082783E-4	4.1799207190733486E-4
31	0.002560862820293039	1.587311665470892E-5	5.2910388849029725E-6	2.4338778870553675E-4	4.1799207190733486E-4
32	0.0029894369699701795	1.587311665470892E-5	5.2910388849029725E-6	2.486788275904397E-4	4.6032038298655866E-4
33	0.0033915559252228056	1.587311665470892E-5	5.2910388849029725E-6	2.592609053602457E-4	4.920666162959765E-4
34	0.0035820333250793125	1.587311665470892E-5	5.2910388849029725E-6	2.592609053602457E-4	5.026486940657825E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	193245	0.0	38.566586	1
AGCAGTG	201775	0.0	36.948086	2
TCAACGC	201855	0.0	36.90372	12
GTGGTAT	201005	0.0	36.868732	6
CAACGCA	202275	0.0	36.840935	13
AGTGGTA	201470	0.0	36.788605	5
ATCAACG	202625	0.0	36.75657	11
AACGCAG	203225	0.0	36.6884	14
ACGCAGA	203395	0.0	36.661667	15
GGTATCA	202540	0.0	36.635727	8
GTATCAA	202585	0.0	36.6345	9
GCAGTGG	203510	0.0	36.583954	3
TGGTATC	202705	0.0	36.568413	7
TATCAAC	203515	0.0	36.50935	10
GAGTACT	108160	0.0	36.455254	20
CGCAGAG	205025	0.0	36.375076	16
GTACTTT	107495	0.0	36.370064	22
AGAGTAC	202950	0.0	36.321262	19
CAGTGGT	204685	0.0	36.223465	4
CAGAGTA	206180	0.0	35.901638	18
>>END_MODULE