FastQCFastQC Report
Thu 26 May 2016
SRR805217_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805217_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22899333
Sequences flagged as poor quality0
Sequence length46
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT5201852.2716163828876588No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCC367380.16043262046104137No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT261640.11425660301983469No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGC249090.10877609404605802No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1768400.037.975571
TCAACGC1861200.036.10788712
CAACGCA1863900.036.07489813
GTGGTAT1861600.035.877746
ATCAACG1873950.035.85794411
ACGCAGA1875450.035.84633315
AACGCAG1876450.035.8272314
AGTGGTA1863750.035.8191835
GGTATCA1868550.035.8085178
GTATCAA1874150.035.723939
TGGTATC1873700.035.666337
TATCAAC1882350.035.59486810
CGCAGAG1890050.035.56519716
AGCAGTG1895300.035.4424132
AGAGTAC1878050.035.39948719
GCAGTGG1905750.035.214483
CAGTGGT1902000.035.1261834
CAGAGTA1904150.035.0308518
GAGTACT661700.034.84660720
GCAGAGT1924350.034.80447817
Produced by FastQC (version 0.11.5)