FastQCFastQC Report
Thu 26 May 2016
SRR805216_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805216_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18825373
Sequences flagged as poor quality0
Sequence length46
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT256750.13638507986003784No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT159950.023.294781
AGCAGTG245800.015.2481692
TCAACGC251000.014.75697212
CAACGCA252850.014.63318213
AGTGGTA257150.014.450715
AACGCAG259650.014.23454714
ACGCAGA261100.014.21677515
GTGGTAT261750.014.173836
ATCAACG262700.014.13779911
GGTATCA265100.013.9645418
GCAGTGG271350.013.8640133
GTATCAA269700.013.815359
TGGTATC272800.013.6290317
TATCAAC273900.013.57429810
CGCAGAG276250.013.49502316
AGAGTAC275950.013.2995119
CAGTGGT285550.013.21664
CAGAGTA298650.012.41587218
GAGTACT102200.012.15264220
GCAGAGT312100.011.96411417
Produced by FastQC (version 0.11.5)