FastQCFastQC Report
Thu 26 May 2016
SRR805215_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805215_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6814964
Sequences flagged as poor quality0
Sequence length46
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT127310.18680949745295794No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT73340.10761612240358129No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT68550.025.8205681
CAACGCA105250.017.50118813
TCAACGC105900.017.41265512
AGCAGTG105700.017.294232
AACGCAG107650.017.16674414
ACGCAGA107700.017.15877515
GCAGTGG107950.016.9893463
GGTATCA108250.016.9053128
GTATCAA109150.016.8208899
TATGCCG6450.016.74418415
AGTGGTA108850.016.7018835
GTGGTAT109800.016.5573776
ATCAACG111450.016.50964511
CTCGTAT6200.016.45161211
CGCAGAG113100.016.41025716
TATCAAC112750.016.24833710
TGGTATC113300.016.08127
AGAGTAC114450.015.93709119
CAGTGGT118150.015.5564964
CAGAGTA121900.015.09433918
Produced by FastQC (version 0.11.5)