##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805215_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6814964
Sequences flagged as poor quality	0
Sequence length	46
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.500792814166005	37.0	35.0	37.0	33.0	37.0
2	35.6689885669242	37.0	35.0	37.0	33.0	37.0
3	37.529411600706915	39.0	37.0	39.0	35.0	39.0
4	37.620727270166064	39.0	38.0	39.0	35.0	39.0
5	37.659260562491596	39.0	38.0	39.0	35.0	39.0
6	37.61553399254934	39.0	38.0	39.0	35.0	39.0
7	37.619167173883824	39.0	37.0	39.0	35.0	39.0
8	38.537246565058894	40.0	39.0	40.0	36.0	40.0
9	38.54607874671091	40.0	39.0	40.0	36.0	40.0
10	38.53079605409508	40.0	39.0	40.0	36.0	40.0
11	38.388649008270626	40.0	38.0	40.0	35.0	40.0
12	38.38968305628614	40.0	38.0	40.0	35.0	40.0
13	38.468547889614676	40.0	39.0	40.0	35.0	40.0
14	38.41292984086196	40.0	39.0	40.0	35.0	40.0
15	38.36249494494762	40.0	38.0	40.0	34.0	40.0
16	38.31135689051329	40.0	38.0	40.0	34.0	40.0
17	38.2577875686504	40.0	38.0	40.0	34.0	40.0
18	38.269703258887354	40.0	38.0	40.0	34.0	40.0
19	38.245803646211485	40.0	38.0	40.0	34.0	40.0
20	38.10298014780415	40.0	38.0	40.0	34.0	40.0
21	38.04530339411917	40.0	38.0	40.0	34.0	40.0
22	37.99565925806798	40.0	38.0	40.0	34.0	40.0
23	37.94373440564029	40.0	38.0	40.0	34.0	40.0
24	37.79012361620693	40.0	38.0	40.0	34.0	40.0
25	37.736822234130656	40.0	38.0	40.0	33.0	40.0
26	37.65605027407335	40.0	38.0	40.0	33.0	40.0
27	37.583149081932056	40.0	38.0	40.0	33.0	40.0
28	37.47888220686126	40.0	37.0	40.0	33.0	40.0
29	37.39404639554956	40.0	37.0	40.0	33.0	40.0
30	37.35862904044688	40.0	37.0	40.0	33.0	40.0
31	37.207137264408146	39.0	37.0	40.0	32.0	40.0
32	37.17656263481363	39.0	37.0	40.0	32.0	40.0
33	37.08464740239273	39.0	37.0	40.0	32.0	40.0
34	36.97320044537286	39.0	36.0	40.0	31.0	40.0
35	36.861195891863844	39.0	36.0	40.0	31.0	40.0
36	36.758878256730334	39.0	36.0	40.0	31.0	40.0
37	36.698557762007255	38.0	36.0	40.0	31.0	40.0
38	36.53743908258356	38.0	36.0	40.0	31.0	40.0
39	36.454064467545244	38.0	35.0	40.0	31.0	40.0
40	36.281243011701896	38.0	35.0	40.0	30.0	40.0
41	36.09651364262526	38.0	35.0	40.0	30.0	40.0
42	35.93463164295512	38.0	35.0	40.0	30.0	40.0
43	35.71272379428564	38.0	35.0	40.0	30.0	40.0
44	35.65715959761489	38.0	34.0	40.0	30.0	40.0
45	35.65934919098619	38.0	34.0	39.0	30.0	40.0
46	36.37845746507245	38.0	36.0	40.0	31.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	8.0
10	23.0
11	37.0
12	89.0
13	201.0
14	400.0
15	678.0
16	1129.0
17	1557.0
18	2279.0
19	3186.0
20	4611.0
21	6687.0
22	9609.0
23	14056.0
24	19943.0
25	26595.0
26	35809.0
27	46791.0
28	58996.0
29	74165.0
30	92437.0
31	113735.0
32	140677.0
33	172571.0
34	217998.0
35	287996.0
36	410913.0
37	685769.0
38	1810474.0
39	2575545.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	15.4788785384633	29.523384129395254	24.077104442517967	30.920632889623484
2	24.131631509718908	25.11797567822809	31.01972365517998	19.730669156873024
3	16.038485309680286	25.745154339773475	32.77854439143039	25.437815959115852
4	17.404596708067714	28.711787765863473	31.98282485424721	21.900790671821596
5	23.305258839224976	28.094880618591677	29.242252783727103	19.357607758456243
6	21.851780875144755	27.010942390891575	30.34905246748185	20.78822426648182
7	22.91219733515834	27.854600552548774	28.914958318195076	20.31824379409781
8	21.2432230016182	26.010291470358464	29.908389831553034	22.838095696470297
9	21.072202288962934	25.46100023419052	31.25232943270133	22.214468044145207
10	21.780878079473347	26.848241604797913	28.895779933687106	22.47510038204164
11	21.68796489607282	27.643491587042867	28.41702465339509	22.251518863489228
12	21.720598964279194	27.092102027244753	28.530671622036447	22.656627386439606
13	22.01184041471092	27.43669665753187	27.369212808754384	23.182250119002827
14	22.405752987103085	27.904784236571167	27.359249439909	22.330213336416744
15	22.06823102807293	27.58148098801402	27.43459833390169	22.915689650011355
16	22.654705145911265	27.187436353295485	27.041654218569604	23.11620428222365
17	23.45465067753843	26.72612797367675	27.11631345374678	22.702907895038038
18	22.38312630851755	27.159688591164972	27.07334917689954	23.383835923417937
19	23.01124407993938	27.45280826134958	26.90995873199037	22.62598892672067
20	23.229278980784052	26.997090520214044	27.143679702489994	22.62995079651191
21	22.868235254067372	27.400218108268803	27.19151561182128	22.540031025842545
22	23.146563943698016	26.746230794469344	27.363167288924785	22.744037972907854
23	22.597727588876477	27.049695346886644	27.87625877407423	22.47631829016265
24	22.425797113528407	27.327011558681747	27.269226954096897	22.97796437369295
25	22.525298739655852	26.902387158611553	27.182329943342324	23.389984158390273
26	22.64421352776038	27.00629086228482	27.844064913622436	22.505430696332365
27	22.819900442614223	26.50710994218018	28.070317025885977	22.60267258931962
28	23.282778896557634	26.585804415107695	27.314817803879816	22.816598884454855
29	23.323659523366523	26.67428617377876	27.422991522772534	22.579062780082186
30	23.06213209636911	26.61192340854625	27.480145162909153	22.84579933217549
31	22.96019465399964	26.730764828691683	27.323959451583306	22.985081065725367
32	23.019079777970948	26.88834453124037	27.371457868302752	22.72111782248593
33	22.821397148979806	26.79949593277382	27.396021461008452	22.983085457237927
34	22.915763017970455	26.928799623886494	27.156519095331976	22.99891826281107
35	22.994750962734358	26.88717064389482	27.2731594767045	22.844918916666323
36	22.862732657135094	26.75870334751585	27.374788773645758	23.0037752217033
37	22.97922630258942	26.906260986851876	27.074933924816037	23.039578785742666
38	23.062308179470943	26.922768777648713	27.284105976201783	22.73081706667856
39	22.8405168391205	26.816487952100697	27.359557585337207	22.983437623441592
40	22.94113365822622	26.878792022965932	27.129475665608798	23.050598653199046
41	22.950143243603343	26.87616545003026	27.369550301366232	22.804141005000172
42	22.82709050260574	26.746993821243958	27.426014282687333	22.999901393462974
43	22.949864445358774	26.805115918440652	27.137751571394947	23.107268064805623
44	22.99174287641138	26.830354496370045	27.28114191065426	22.896760716564312
45	22.906768106185154	26.680551797485652	27.410944503888796	23.0017355924404
46	22.962542428690746	26.78521852793353	27.130869656831642	23.12136938654408
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2036.0
1	1493.0
2	950.0
3	862.0
4	774.0
5	880.5
6	987.0
7	987.0
8	1358.5
9	1730.0
10	2531.5
11	3333.0
12	5065.5
13	6798.0
14	9395.5
15	11993.0
16	17387.0
17	22781.0
18	31145.5
19	39510.0
20	39510.0
21	52286.5
22	65063.0
23	81984.5
24	98906.0
25	127287.0
26	155668.0
27	164426.5
28	173185.0
29	192389.0
30	211593.0
31	211593.0
32	235744.5
33	259896.0
34	287367.0
35	314838.0
36	342746.0
37	370654.0
38	398519.0
39	426384.0
40	449015.0
41	471646.0
42	489851.5
43	508057.0
44	508057.0
45	518902.0
46	529747.0
47	532303.5
48	534860.0
49	525459.5
50	516059.0
51	497152.0
52	478245.0
53	451127.0
54	424009.0
55	388560.5
56	353112.0
57	353112.0
58	316507.0
59	279902.0
60	243650.0
61	207398.0
62	175333.5
63	143269.0
64	117273.5
65	91278.0
66	73074.5
67	54871.0
68	42107.0
69	29343.0
70	29343.0
71	21760.5
72	14178.0
73	10368.0
74	6558.0
75	4796.5
76	3035.0
77	2162.0
78	1289.0
79	919.0
80	549.0
81	549.0
82	432.5
83	316.0
84	198.0
85	80.0
86	76.5
87	73.0
88	39.5
89	6.0
90	4.5
91	3.0
92	2.0
93	1.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
46	6814964.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.322770387297744
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.75272402038811	50.327334772714714
2	10.993631814755654	13.703071826270161
3	3.216218193057853	6.013308838841827
4	1.4540454696701197	3.6248056775576547
5	0.8737971663450032	2.722873008159552
6	0.537059976394584	2.008263935582833
7	0.3778888668995237	1.6485756758586594
8	0.28082461960122407	1.4001414629205857
9	0.22925738049031796	1.2859159575502295
>10	1.2070009894429439	13.234243776756353
>50	0.06717291074573105	2.8009094312309126
>100	0.010212710973719008	0.8338061403757664
>500	4.739473057183845E-5	0.024826441846796696
>1k	7.109190282162081E-5	0.07558679236948429
>5k	2.3697300937492557E-5	0.10831448518554408
>10k+	2.3697300937492557E-5	0.1880217767789967
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	12731	0.18680949745295794	No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	7334	0.10761612240358129	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.4673591819413867E-4	0.0	1.4673591819413866E-5	0.0	1.4673591819413866E-5
2	1.9075669365238026E-4	0.0	1.4673591819413866E-5	0.0	1.4673591819413866E-5
3	2.0543028547179412E-4	0.0	1.4673591819413866E-5	0.0	1.4673591819413866E-5
4	2.0543028547179412E-4	0.0	1.4673591819413866E-5	0.0	1.4673591819413866E-5
5	5.575964891377269E-4	0.0	1.4673591819413866E-5	0.0	1.4673591819413866E-5
6	5.722700809571408E-4	0.0	1.4673591819413866E-5	0.0	1.4673591819413866E-5
7	6.016172645959685E-4	0.0	1.4673591819413866E-5	0.0	2.9347183638827732E-5
8	6.162908564153824E-4	0.0	1.4673591819413866E-5	0.0	2.9347183638827732E-5
9	6.162908564153824E-4	0.0	2.9347183638827732E-5	0.0	4.40207754582416E-5
10	6.456380400542101E-4	0.0	2.9347183638827732E-5	0.0	5.8694367277655464E-5
11	7.043324073318656E-4	0.0	2.9347183638827732E-5	0.0	5.8694367277655464E-5
12	8.657419173454181E-4	0.0	2.9347183638827732E-5	1.4673591819413866E-5	7.336795909706933E-5
13	0.0010124778355395567	0.0	2.9347183638827732E-5	1.4673591819413866E-5	7.336795909706933E-5
14	0.0010711722028172123	0.0	2.9347183638827732E-5	1.4673591819413866E-5	7.336795909706933E-5
15	0.001188560937372523	0.0	2.9347183638827732E-5	2.9347183638827732E-5	8.80415509164832E-5
16	0.0012325817128307649	0.0	2.9347183638827732E-5	5.8694367277655464E-5	1.0271514273589706E-4
17	0.0013499704473860757	0.0	2.9347183638827732E-5	5.8694367277655464E-5	1.320623263747248E-4
18	0.0014086648146637312	0.0	2.9347183638827732E-5	7.336795909706933E-5	1.320623263747248E-4
19	0.0014673591819413865	0.0	2.9347183638827732E-5	8.80415509164832E-5	1.320623263747248E-4
20	0.0014967063655802143	2.9347183638827732E-5	2.9347183638827732E-5	8.80415509164832E-5	1.320623263747248E-4
21	0.0015700743246772836	2.9347183638827732E-5	2.9347183638827732E-5	8.80415509164832E-5	1.4673591819413867E-4
22	0.0015994215083161114	2.9347183638827732E-5	2.9347183638827732E-5	8.80415509164832E-5	1.4673591819413867E-4
23	0.001643442283774353	2.9347183638827732E-5	4.40207754582416E-5	8.80415509164832E-5	1.6140951001355252E-4
24	0.0016874630592325947	2.9347183638827732E-5	5.8694367277655464E-5	8.80415509164832E-5	1.760831018329664E-4
25	0.0017168102428714224	2.9347183638827732E-5	5.8694367277655464E-5	8.80415509164832E-5	1.760831018329664E-4
26	0.0017755046101490778	2.9347183638827732E-5	5.8694367277655464E-5	8.80415509164832E-5	1.760831018329664E-4
27	0.001848872569246147	4.40207754582416E-5	5.8694367277655464E-5	8.80415509164832E-5	1.760831018329664E-4
28	0.001863546161065561	4.40207754582416E-5	5.8694367277655464E-5	8.80415509164832E-5	1.760831018329664E-4
29	0.0018782197528849748	4.40207754582416E-5	5.8694367277655464E-5	8.80415509164832E-5	1.760831018329664E-4
30	0.0019369141201626304	4.40207754582416E-5	5.8694367277655464E-5	8.80415509164832E-5	1.760831018329664E-4
31	0.0020102820792597	5.8694367277655464E-5	5.8694367277655464E-5	8.80415509164832E-5	1.760831018329664E-4
32	0.0020543028547179414	7.336795909706933E-5	5.8694367277655464E-5	8.80415509164832E-5	1.760831018329664E-4
33	0.002186365181092666	7.336795909706933E-5	5.8694367277655464E-5	8.80415509164832E-5	1.9075669365238026E-4
34	0.0022303859565509076	8.80415509164832E-5	5.8694367277655464E-5	8.80415509164832E-5	1.9075669365238026E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	6855	0.0	25.820568	1
CAACGCA	10525	0.0	17.501188	13
TCAACGC	10590	0.0	17.412655	12
AGCAGTG	10570	0.0	17.29423	2
AACGCAG	10765	0.0	17.166744	14
ACGCAGA	10770	0.0	17.158775	15
GCAGTGG	10795	0.0	16.989346	3
GGTATCA	10825	0.0	16.905312	8
GTATCAA	10915	0.0	16.820889	9
TATGCCG	645	0.0	16.744184	15
AGTGGTA	10885	0.0	16.701883	5
GTGGTAT	10980	0.0	16.557377	6
ATCAACG	11145	0.0	16.509645	11
CTCGTAT	620	0.0	16.451612	11
CGCAGAG	11310	0.0	16.410257	16
TATCAAC	11275	0.0	16.248337	10
TGGTATC	11330	0.0	16.0812	7
AGAGTAC	11445	0.0	15.937091	19
CAGTGGT	11815	0.0	15.556496	4
CAGAGTA	12190	0.0	15.094339	18
>>END_MODULE