FastQCFastQC Report
Thu 26 May 2016
SRR805214_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805214_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16477769
Sequences flagged as poor quality0
Sequence length46
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT1070780.6498331175779924No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT194170.11783755434367359No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT414200.034.495411
CAACGCA490450.029.1895213
TCAACGC491500.029.14343812
GTGGTAT490850.029.1168386
AGTGGTA491700.029.0990455
GGTATCA494600.028.8920358
AACGCAG497700.028.82459314
ACGCAGA498750.028.7478715
TGGTATC496900.028.7462277
ATCAACG502200.028.55436111
GTATCAA501400.028.4922229
TATCAAC506100.028.22367110
CGCAGAG508950.028.21888216
CAGTGGT513150.027.9099674
AGCAGTG512400.027.80642
AGAGTAC506550.027.78797719
GCAGTGG518800.027.5212043
CAGAGTA528300.026.82188218
GCAGAGT539550.026.3627117
ACATGGG353550.026.03309424
Produced by FastQC (version 0.11.5)