##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805214_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	16477769
Sequences flagged as poor quality	0
Sequence length	46
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.91268824074424	37.0	35.0	37.0	35.0	37.0
2	35.81011634524067	37.0	35.0	37.0	35.0	37.0
3	37.56316167558848	39.0	37.0	39.0	35.0	39.0
4	37.664214251334634	39.0	37.0	39.0	35.0	39.0
5	37.719177395920525	39.0	37.0	39.0	35.0	39.0
6	37.687474863860515	39.0	37.0	39.0	35.0	39.0
7	37.66394334087339	39.0	37.0	39.0	35.0	39.0
8	38.62792808905137	40.0	39.0	40.0	36.0	40.0
9	38.66361744724058	40.0	39.0	40.0	36.0	40.0
10	38.592566020315004	40.0	39.0	40.0	36.0	40.0
11	38.61292083898008	40.0	39.0	40.0	36.0	40.0
12	38.599917561655346	40.0	39.0	40.0	36.0	40.0
13	38.63295207014979	40.0	39.0	40.0	36.0	40.0
14	38.52036650107184	40.0	39.0	40.0	35.0	40.0
15	38.485132422963325	40.0	39.0	40.0	35.0	40.0
16	38.52051712825929	40.0	39.0	40.0	35.0	40.0
17	38.52057569201268	40.0	39.0	40.0	35.0	40.0
18	38.51467106985175	40.0	39.0	40.0	35.0	40.0
19	38.43180281262591	40.0	38.0	40.0	35.0	40.0
20	38.333653239100514	40.0	38.0	40.0	35.0	40.0
21	38.25593592190788	40.0	38.0	40.0	34.0	40.0
22	38.185578520975746	40.0	38.0	40.0	34.0	40.0
23	37.97340119284352	40.0	38.0	40.0	34.0	40.0
24	38.00604693511603	40.0	38.0	40.0	34.0	40.0
25	38.03667602088608	40.0	38.0	40.0	34.0	40.0
26	37.95526560664857	40.0	38.0	40.0	34.0	40.0
27	37.882364718184846	40.0	38.0	40.0	34.0	40.0
28	37.777813307129136	40.0	38.0	40.0	33.0	40.0
29	37.69445220405748	40.0	38.0	40.0	33.0	40.0
30	37.67244594823486	40.0	38.0	40.0	33.0	40.0
31	37.58189327693573	40.0	38.0	40.0	33.0	40.0
32	37.52859922966513	40.0	37.0	40.0	33.0	40.0
33	37.45674781579958	40.0	37.0	40.0	33.0	40.0
34	37.37703368702402	40.0	37.0	40.0	33.0	40.0
35	37.309082558445866	40.0	37.0	40.0	32.0	40.0
36	37.23924209642701	40.0	37.0	40.0	32.0	40.0
37	37.150974139763704	39.0	37.0	40.0	32.0	40.0
38	36.986644308461905	39.0	36.0	40.0	31.0	40.0
39	36.993407359940534	39.0	36.0	40.0	31.0	40.0
40	36.86270259038102	39.0	36.0	40.0	31.0	40.0
41	36.693000551227534	39.0	36.0	40.0	31.0	40.0
42	36.55611763946928	39.0	36.0	40.0	31.0	40.0
43	36.40234846112966	38.0	35.0	40.0	30.0	40.0
44	36.35346738991183	38.0	35.0	40.0	30.0	40.0
45	36.23245021823039	38.0	35.0	40.0	30.0	40.0
46	36.46756863747756	39.0	35.0	40.0	30.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	2.0
12	11.0
13	21.0
14	78.0
15	278.0
16	696.0
17	1320.0
18	2246.0
19	3546.0
20	5930.0
21	9727.0
22	15804.0
23	24709.0
24	36573.0
25	52368.0
26	71808.0
27	95931.0
28	124508.0
29	159463.0
30	200516.0
31	250047.0
32	312285.0
33	389263.0
34	497143.0
35	661008.0
36	940819.0
37	1536747.0
38	3432560.0
39	7652361.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.534862698949112	39.5178861895685	15.623146555823181	20.324104555659204
2	30.20135189417936	29.48263202378914	25.708996163254866	14.607019918776626
3	24.118568478536144	24.790631547268323	26.53113416021307	24.559665813982463
4	21.35605857807571	30.615995405688718	24.314031832828825	23.713914183406747
5	22.159911332656744	30.27184687441607	23.9410990650494	23.627142727877786
6	23.81986299237476	25.29776330764195	28.487030009948555	22.395343690034736
7	22.060019168857142	26.495674262699033	27.399152154639385	24.045154413804443
8	23.353634827627452	25.399470037478984	27.335721237504906	23.911173897388657
9	24.33210466781031	25.82698543716689	26.987785785806317	22.853124109216484
10	22.262091427547016	27.309801466448526	28.356411599167338	22.071695506837123
11	22.149418407309874	28.891987744214642	26.89788283838668	22.060711010088806
12	22.22957488965891	25.987917417703816	28.667017968269853	23.115489724367418
13	22.48448197082991	26.821737821424733	26.063552656916116	24.630227550829243
14	22.55594795630404	28.679671380269987	26.162516296957435	22.601864366468543
15	22.6588077548605	27.992776206536213	25.95593493269629	23.392481105906995
16	23.363035372082226	26.202479231260007	25.56543910768503	24.869046288972736
17	25.0467523849861	26.045528372196504	25.799475645034224	23.108243597783172
18	22.84555633714734	26.15163496951559	25.72625578135001	25.276552911987054
19	23.58501930692195	28.216574707413365	25.392612312989705	22.805793672674984
20	25.11749618531489	26.40990415632116	25.750069684797744	22.722529973566203
21	23.485776502874874	27.849304113924646	25.648004896779415	23.01691448642107
22	25.217843507819538	26.07962279359542	25.879680677645133	22.82285302093991
23	23.1596097748427	26.284984332527056	27.92912681322332	22.626279079406928
24	23.011088454996546	27.770707308738217	25.697180243272012	23.521023992993225
25	23.116897681961678	26.29361414157463	25.552973827949643	25.03651434851405
26	23.041662982409818	27.309965323582336	26.95258684594984	22.69578484805801
27	23.380877593319823	25.51319295712909	27.98336352451597	23.12256592503512
28	24.84255605233937	26.047142668403716	26.23876448322585	22.871536796031062
29	24.858201374227303	25.911930189092953	26.495765294440044	22.734103142239707
30	24.636684735658086	25.63203186062385	26.52386375849789	23.207419645220178
31	24.28714105653502	26.25635181558863	26.29063436925229	23.165872758624058
32	23.715413172742014	26.42846856270409	26.700058727610514	23.15605953694338
33	23.62679073847922	25.97698147121737	26.701873293647942	23.694354496655464
34	23.74219471094661	26.358974931618473	26.487572437749307	23.411257919685607
35	23.524816982201898	26.305515024515756	26.81994753051824	23.349720462764104
36	23.620454929305055	25.93266721969461	26.74849368260958	23.698384168390756
37	23.798009305750067	26.34453729749458	26.470689084183668	23.386764312571685
38	23.70842800381532	26.266614127191612	26.773946157395457	23.25101171159761
39	23.6902762746583	25.94362137252925	26.72009177941504	23.646010573397405
40	23.78622372968088	26.41435257406509	26.365335015923574	23.43408868033045
41	23.634904700994415	26.395084188885036	26.758331179421198	23.211679930699354
42	23.701011951314527	26.007343591234957	26.697801140433512	23.593843317017008
43	23.855225789365054	26.387279734289272	26.33798908092473	23.419505395420945
44	23.810529204530052	26.246156260595715	26.66157657629501	23.281737958579225
45	23.74313537227036	25.87349051925658	26.676560400864947	23.706813707608113
46	23.862890661957938	26.281428025845006	26.31879958992021	23.536881722276846
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4502.0
1	3283.0
2	2064.0
3	1904.0
4	1744.0
5	1955.0
6	2166.0
7	2166.0
8	2843.0
9	3520.0
10	4823.5
11	6127.0
12	9349.5
13	12572.0
14	16877.5
15	21183.0
16	29497.0
17	37811.0
18	52810.5
19	67810.0
20	67810.0
21	89601.5
22	111393.0
23	140697.5
24	170002.0
25	265886.0
26	361770.0
27	336948.5
28	312127.0
29	348786.0
30	385445.0
31	385445.0
32	439406.5
33	493368.0
34	553606.0
35	613844.0
36	681403.5
37	748963.0
38	820149.5
39	891336.0
40	964804.5
41	1038273.0
42	1105897.0
43	1173521.0
44	1173521.0
45	1231090.0
46	1288659.0
47	1317243.5
48	1345828.0
49	1350153.5
50	1354479.0
51	1326427.0
52	1298375.0
53	1239324.5
54	1180274.0
55	1101436.0
56	1022598.0
57	1022598.0
58	922689.5
59	822781.0
60	720493.0
61	618205.0
62	528031.5
63	437858.0
64	361589.5
65	285321.0
66	230969.0
67	176617.0
68	137095.5
69	97574.0
70	97574.0
71	72494.5
72	47415.0
73	34842.0
74	22269.0
75	16544.0
76	10819.0
77	7793.5
78	4768.0
79	3504.0
80	2240.0
81	2240.0
82	1728.0
83	1216.0
84	903.5
85	591.0
86	417.0
87	243.0
88	150.5
89	58.0
90	42.5
91	27.0
92	17.0
93	7.0
94	7.0
95	4.5
96	2.0
97	3.0
98	4.0
99	2.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
46	1.6477769E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.29894341639477
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.7065290095609	30.879255843478187
2	15.009998257337532	13.298541269639589
3	5.560677947470344	7.389964732553489
4	2.80305209401784	4.966889844244126
5	1.5392644314633812	3.4093893976132694
6	0.99532102710369	2.645500191648857
7	0.6728960419366643	2.0866008580807804
8	0.5045114737541843	1.7879460183006834
9	0.3959164694334417	1.5784813149345742
>10	2.5791730026574733	21.96842599851993
>50	0.1850684553093154	5.4742318230912135
>100	0.04629626636810399	3.221524715204268
>500	0.0010970393652523574	0.3095458169673835
>1k	1.4178288839989086E-4	0.11676380265115902
>5k	2.8350666718698064E-5	0.07261012333829707
>10k+	2.8350666718698064E-5	0.7943282497343153
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	107078	0.6498331175779924	No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	19417	0.11783755434367359	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.7599469928240892E-4	0.0	0.0	0.0	0.0
2	1.8813226475016125E-4	6.068782733876169E-6	6.068782733876169E-6	0.0	0.0
3	2.002698302179136E-4	6.068782733876169E-6	6.068782733876169E-6	0.0	6.068782733876169E-6
4	2.0633861295178978E-4	6.068782733876169E-6	6.068782733876169E-6	0.0	1.2137565467752339E-5
5	2.184761784195421E-4	6.068782733876169E-6	6.068782733876169E-6	0.0	2.4275130935504677E-5
6	2.3061374388729446E-4	6.068782733876169E-6	1.2137565467752339E-5	0.0	3.034391366938085E-5
7	2.791640057583038E-4	6.068782733876169E-6	1.2137565467752339E-5	0.0	3.034391366938085E-5
8	3.0950791942768464E-4	6.068782733876169E-6	1.2137565467752339E-5	6.068782733876169E-6	3.641269640325702E-5
9	3.155767021615608E-4	6.068782733876169E-6	1.2137565467752339E-5	6.068782733876169E-6	4.248147913713319E-5
10	3.155767021615608E-4	6.068782733876169E-6	1.2137565467752339E-5	1.820634820162851E-5	4.8550261871009355E-5
11	3.277142676293132E-4	6.068782733876169E-6	1.2137565467752339E-5	1.820634820162851E-5	5.461904460488553E-5
12	3.459206158309417E-4	6.068782733876169E-6	1.2137565467752339E-5	1.820634820162851E-5	6.06878273387617E-5
13	3.641269640325702E-4	6.068782733876169E-6	1.2137565467752339E-5	1.820634820162851E-5	6.675661007263787E-5
14	3.641269640325702E-4	6.068782733876169E-6	1.2137565467752339E-5	2.4275130935504677E-5	6.675661007263787E-5
15	3.8840209496807484E-4	6.068782733876169E-6	1.2137565467752339E-5	2.4275130935504677E-5	6.675661007263787E-5
16	4.187460086374557E-4	6.068782733876169E-6	1.2137565467752339E-5	2.4275130935504677E-5	9.710052374201871E-5
17	4.369523568390842E-4	6.068782733876169E-6	1.2137565467752339E-5	2.4275130935504677E-5	1.0923808920977105E-4
18	4.4908992230683654E-4	1.2137565467752339E-5	1.2137565467752339E-5	3.034391366938085E-5	1.1530687194364723E-4
19	4.855026187100936E-4	1.2137565467752339E-5	1.2137565467752339E-5	3.034391366938085E-5	1.6992591654853276E-4
20	5.219153151133506E-4	1.2137565467752339E-5	1.2137565467752339E-5	3.641269640325702E-5	1.820634820162851E-4
21	5.583280115166076E-4	1.2137565467752339E-5	1.2137565467752339E-5	3.641269640325702E-5	1.9420104748403742E-4
22	5.886719251859885E-4	1.820634820162851E-5	1.2137565467752339E-5	3.641269640325702E-5	2.0633861295178978E-4
23	6.372221870569978E-4	1.820634820162851E-5	1.2137565467752339E-5	3.641269640325702E-5	2.0633861295178978E-4
24	6.675661007263787E-4	1.820634820162851E-5	1.2137565467752339E-5	3.641269640325702E-5	2.2454496115341827E-4
25	7.039787971296357E-4	1.820634820162851E-5	1.2137565467752339E-5	4.248147913713319E-5	2.427513093550468E-4
26	7.707354072022736E-4	1.820634820162851E-5	1.2137565467752339E-5	4.248147913713319E-5	2.5488887482279915E-4
27	8.314232345410352E-4	1.820634820162851E-5	1.2137565467752339E-5	4.248147913713319E-5	2.5488887482279915E-4
28	9.224549755491779E-4	1.820634820162851E-5	1.2137565467752339E-5	4.248147913713319E-5	2.791640057583038E-4
29	0.0010134867165573203	1.820634820162851E-5	1.2137565467752339E-5	4.248147913713319E-5	2.9130157122605614E-4
30	0.001146999936702596	1.820634820162851E-5	1.2137565467752339E-5	4.248147913713319E-5	2.9130157122605614E-4
31	0.0012623068086462432	1.820634820162851E-5	1.2137565467752339E-5	4.248147913713319E-5	2.9130157122605614E-4
32	0.0014504390733964046	1.820634820162851E-5	1.820634820162851E-5	4.248147913713319E-5	3.0950791942768464E-4
33	0.0016507089036143182	1.820634820162851E-5	1.820634820162851E-5	4.248147913713319E-5	3.21645484895437E-4
34	0.0017781533410257178	1.820634820162851E-5	1.820634820162851E-5	4.248147913713319E-5	3.21645484895437E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	41420	0.0	34.49541	1
CAACGCA	49045	0.0	29.18952	13
TCAACGC	49150	0.0	29.143438	12
GTGGTAT	49085	0.0	29.116838	6
AGTGGTA	49170	0.0	29.099045	5
GGTATCA	49460	0.0	28.892035	8
AACGCAG	49770	0.0	28.824593	14
ACGCAGA	49875	0.0	28.74787	15
TGGTATC	49690	0.0	28.746227	7
ATCAACG	50220	0.0	28.554361	11
GTATCAA	50140	0.0	28.492222	9
TATCAAC	50610	0.0	28.223671	10
CGCAGAG	50895	0.0	28.218882	16
CAGTGGT	51315	0.0	27.909967	4
AGCAGTG	51240	0.0	27.8064	2
AGAGTAC	50655	0.0	27.787977	19
GCAGTGG	51880	0.0	27.521204	3
CAGAGTA	52830	0.0	26.821882	18
GCAGAGT	53955	0.0	26.36271	17
ACATGGG	35355	0.0	26.033094	24
>>END_MODULE