FastQCFastQC Report
Thu 26 May 2016
SRR805213_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805213_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20195690
Sequences flagged as poor quality0
Sequence length46
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT263400.13042386766681408No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT156250.021.29921
AGCAGTG241750.013.8407452
TCAACGC250500.013.45309412
AGTGGTA253550.013.4016965
CAACGCA252000.013.38095313
AACGCAG257450.013.15206914
ACGCAGA260700.012.98043815
GTGGTAT262550.012.9422966
ATCAACG261700.012.93083711
GGTATCA267550.012.6331528
GCAGTGG269900.012.6194893
GTATCAA269600.012.5890219
TATCAAC273000.012.38827810
CGCAGAG276700.012.30213216
AGAGTAC274900.012.14987319
TGGTATC279000.012.1218637
CAGTGGT288600.011.9126824
GAGTACT107450.011.18659920
CAGAGTA306300.011.07411118
GCAGAGT313900.010.90793217
Produced by FastQC (version 0.11.5)