##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805213_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	20195690
Sequences flagged as poor quality	0
Sequence length	46
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.94847960134068	37.0	35.0	37.0	35.0	37.0
2	35.65978047791385	37.0	35.0	37.0	35.0	37.0
3	37.41087722182307	39.0	37.0	39.0	35.0	39.0
4	37.5728327182681	39.0	37.0	39.0	35.0	39.0
5	37.6312159673673	39.0	37.0	39.0	35.0	39.0
6	37.58568427223828	39.0	37.0	39.0	35.0	39.0
7	37.596361104770374	39.0	37.0	39.0	35.0	39.0
8	38.55127797069573	40.0	39.0	40.0	36.0	40.0
9	38.58390517976856	40.0	39.0	40.0	36.0	40.0
10	38.592725923204405	40.0	39.0	40.0	36.0	40.0
11	38.57572358260599	40.0	39.0	40.0	36.0	40.0
12	38.560273999056236	40.0	39.0	40.0	36.0	40.0
13	38.5788967348974	40.0	39.0	40.0	36.0	40.0
14	38.45208710373352	40.0	39.0	40.0	35.0	40.0
15	38.43130712543122	40.0	39.0	40.0	35.0	40.0
16	38.476544450820946	40.0	39.0	40.0	35.0	40.0
17	38.47690551795952	40.0	39.0	40.0	35.0	40.0
18	38.47707738631362	40.0	39.0	40.0	35.0	40.0
19	38.377554517820386	40.0	38.0	40.0	35.0	40.0
20	38.26755575075672	40.0	38.0	40.0	34.0	40.0
21	38.21054239790767	40.0	38.0	40.0	34.0	40.0
22	38.15523361667762	40.0	38.0	40.0	34.0	40.0
23	38.036947487310414	40.0	38.0	40.0	34.0	40.0
24	38.017110482484135	40.0	38.0	40.0	34.0	40.0
25	38.015866751767334	40.0	38.0	40.0	34.0	40.0
26	37.94322565854397	40.0	38.0	40.0	34.0	40.0
27	37.87328494347061	40.0	38.0	40.0	34.0	40.0
28	37.74973308661402	40.0	38.0	40.0	33.0	40.0
29	37.64869053743645	40.0	38.0	40.0	33.0	40.0
30	37.638819421371586	40.0	38.0	40.0	33.0	40.0
31	37.57882513546207	40.0	37.0	40.0	33.0	40.0
32	37.525406757580456	40.0	37.0	40.0	33.0	40.0
33	37.468965655543336	40.0	37.0	40.0	33.0	40.0
34	37.39183192057315	40.0	37.0	40.0	33.0	40.0
35	37.31369698188079	40.0	37.0	40.0	32.0	40.0
36	37.257599864129425	40.0	37.0	40.0	32.0	40.0
37	37.182058201527155	40.0	37.0	40.0	32.0	40.0
38	37.016932226628555	39.0	37.0	40.0	31.0	40.0
39	37.02587819480295	39.0	36.0	40.0	31.0	40.0
40	36.89655669105636	39.0	36.0	40.0	31.0	40.0
41	36.716485893772386	39.0	36.0	40.0	31.0	40.0
42	36.60379912743759	39.0	36.0	40.0	31.0	40.0
43	36.46703554075152	39.0	35.0	40.0	30.0	40.0
44	36.403524365842415	38.0	35.0	40.0	30.0	40.0
45	36.28298691453473	38.0	35.0	40.0	30.0	40.0
46	36.461674644441466	39.0	35.0	40.0	30.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	4.0
13	31.0
14	86.0
15	350.0
16	839.0
17	1748.0
18	2971.0
19	4935.0
20	8046.0
21	13231.0
22	21375.0
23	32582.0
24	47773.0
25	67369.0
26	91784.0
27	121678.0
28	157694.0
29	200040.0
30	251680.0
31	313969.0
32	389947.0
33	486534.0
34	618538.0
35	822552.0
36	1156565.0
37	1855887.0
38	4039352.0
39	9488129.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	24.027483091689366	28.572447883682113	22.323728478700158	25.076340545928367
2	25.902714886196016	23.26253769987557	28.581053680265445	22.253693733662974
3	20.677525749305918	25.409094712782775	27.540762410197424	26.37261712771388
4	22.12821151443699	28.49137117870199	26.582800587650134	22.797616719210883
5	25.682544146795678	27.300904301858463	25.112561145472128	21.903990405873728
6	24.333360236763387	25.826604587414444	27.132779320736255	22.70725585508591
7	24.1210079972509	26.70154374522485	26.644526629196623	22.532921628327628
8	23.224930665899507	25.811205262112857	27.162260858628745	23.80160321335889
9	22.957333965811515	25.78085720270018	27.819024752311012	23.44278407917729
10	23.013306304463974	26.52149047643334	27.198441845760158	23.26676137334253
11	22.928258455145627	26.814894663168232	26.94460550741272	23.31224137427342
12	22.959096718161152	26.31566438185573	26.97709263709237	23.748146262890746
13	23.198806279953793	26.664392253990826	26.28752966598319	23.849271800072195
14	23.356572615246122	26.859844848083924	26.380217759333796	23.403364777336154
15	23.357770890719753	26.443305477554862	26.30343404954225	23.89548958218313
16	23.657745786353427	26.392269835791698	26.097726792201705	23.852257585653177
17	24.092130548646765	26.187122103775607	26.191063538804567	23.52968380877306
18	23.464996739403308	26.30556321670614	26.217697934559304	24.011742109331248
19	24.001477542980705	26.594199059304234	25.99758166222595	23.406741735489106
20	24.011548998821038	26.235781000797697	26.31615458545858	23.43651541492269
21	23.73595554298962	26.422622846755917	26.247620160539203	23.59380144971526
22	24.001116079718	26.09528567729055	26.320244567033857	23.583353675957593
23	23.7135398691503	26.2615439234807	26.63629219897909	23.388624008389908
24	23.506515499099066	26.376325839820282	26.290059908822126	23.82709875225853
25	23.756875848262673	26.151728413339676	26.2070768564976	23.88431888190005
26	23.823201881193462	26.228447752961152	26.634252159742992	23.31409820610239
27	23.931115995541624	25.81448318923493	26.653370100254065	23.601030714969383
28	24.262275762798893	26.02171552445101	26.205036817261508	23.51097189548859
29	24.24550485771964	26.01361478612516	26.365957290887316	23.374923065267886
30	23.99547131095793	25.88001697391869	26.38488212088817	23.739629594235208
31	24.0272503687668	26.139745658603395	26.22119868150086	23.61180529112895
32	23.885145791007883	26.14400894448271	26.35443007889307	23.616415185616336
33	23.795517756511416	25.972130687290207	26.344690377006184	23.887661179192193
34	23.952853306819424	26.153392134658432	26.204338648493813	23.689415910028327
35	23.87069716360273	26.153674373096436	26.311331774254803	23.66429668904603
36	23.83252565275066	25.981107850239333	26.2834050235471	23.902961473462902
37	23.98009179186252	26.199931767619727	26.09987081402022	23.720105626497535
38	23.93709251825513	26.169648078377115	26.268956396141952	23.624303007225798
39	23.862210204256453	25.994754326294373	26.279904276605553	23.863131192843625
40	24.010855781604885	26.163651749457433	26.087675142567544	23.73781732637013
41	23.884818988605986	26.15747221313062	26.29907173263206	23.65863706563133
42	23.875277348780855	25.946749034076085	26.31929386913742	23.858679748005642
43	24.01696104465854	26.114680904688083	26.051712023704066	23.816646026949314
44	24.028364467864183	26.037753599901762	26.255453515081683	23.67842841715237
45	23.912191165540765	25.874223658612305	26.276557027761864	23.937028148085062
46	24.042654645619933	26.092864368585573	26.050949484766306	23.81353150102819
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	10063.0
1	6650.5
2	3238.0
3	2881.0
4	2524.0
5	2745.5
6	2967.0
7	2967.0
8	3598.5
9	4230.0
10	5558.0
11	6886.0
12	10386.0
13	13886.0
14	18807.5
15	23729.0
16	33214.0
17	42699.0
18	59902.5
19	77106.0
20	77106.0
21	100970.5
22	124835.0
23	158287.5
24	191740.0
25	250022.5
26	308305.0
27	327743.5
28	347182.0
29	393129.0
30	439076.0
31	439076.0
32	501484.0
33	563892.0
34	636337.0
35	708782.0
36	795034.5
37	881287.0
38	971205.0
39	1061123.0
40	1154514.0
41	1247905.0
42	1340658.5
43	1433412.0
44	1433412.0
45	1509262.0
46	1585112.0
47	1626212.5
48	1667313.0
49	1677810.5
50	1688308.0
51	1659886.5
52	1631465.0
53	1561324.5
54	1491184.0
55	1393547.0
56	1295910.0
57	1295910.0
58	1178868.5
59	1061827.0
60	934604.5
61	807382.0
62	692816.0
63	578250.0
64	480504.5
65	382759.0
66	312138.5
67	241518.0
68	188461.5
69	135405.0
70	135405.0
71	102261.0
72	69117.0
73	51697.5
74	34278.0
75	25768.0
76	17258.0
77	12465.0
78	7672.0
79	5555.0
80	3438.0
81	3438.0
82	2476.5
83	1515.0
84	1092.0
85	669.0
86	514.0
87	359.0
88	204.5
89	50.0
90	36.5
91	23.0
92	16.0
93	9.0
94	9.0
95	5.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
46	2.019569E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.72342756940958
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.48307777684593	28.990721629218836
2	14.600733961304243	12.183853317893924
3	5.444966294755001	6.815479704512602
4	2.756327777605257	4.600137695458584
5	1.6570752465456335	3.456942951315413
6	1.0257843557524355	2.567954356144213
7	0.7443568027904621	2.1739982002917593
8	0.5609269793433701	1.8723036955488646
9	0.4399108921450779	1.651913122086856
>10	2.991491290714183	25.074661265255788
>50	0.23872095306141505	6.618863307638316
>100	0.056082997302443004	3.538668513437604
>500	4.0951301722989077E-4	0.10710509070088574
>1k	8.604509414300464E-5	0.06406037519829684
>5k	2.4556861581449116E-5	0.07505681188442849
>10k+	2.4556861581449116E-5	0.2082799634136458
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	26340	0.13042386766681408	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.3272292256417088E-4	0.0	0.0	0.0	1.9806206175674118E-5
2	2.52529128739845E-4	0.0	0.0	0.0	1.9806206175674118E-5
3	2.52529128739845E-4	0.0	0.0	0.0	1.9806206175674118E-5
4	2.52529128739845E-4	4.9515515439185296E-6	0.0	0.0	2.9709309263511176E-5
5	2.52529128739845E-4	4.9515515439185296E-6	0.0	0.0	3.46608608074297E-5
6	2.52529128739845E-4	4.9515515439185296E-6	0.0	0.0	4.9515515439185294E-5
7	2.574806802837635E-4	4.9515515439185296E-6	0.0	0.0	5.446706698310382E-5
8	2.574806802837635E-4	4.9515515439185296E-6	0.0	0.0	5.941861852702235E-5
9	2.574806802837635E-4	4.9515515439185296E-6	0.0	0.0	6.93217216148594E-5
10	2.6243223182768205E-4	4.9515515439185296E-6	0.0	0.0	7.427327315877793E-5
11	2.6243223182768205E-4	4.9515515439185296E-6	0.0	4.9515515439185296E-6	8.4176376246615E-5
12	2.723353349155191E-4	4.9515515439185296E-6	0.0	4.9515515439185296E-6	9.407947933445206E-5
13	2.8223843800335616E-4	9.903103087837059E-6	0.0	4.9515515439185296E-6	1.2378878859796322E-4
14	2.970930926351117E-4	9.903103087837059E-6	0.0	4.9515515439185296E-6	1.386434432297188E-4
15	3.020446441790303E-4	9.903103087837059E-6	0.0	4.9515515439185296E-6	1.4359499477363733E-4
16	3.218508503547044E-4	9.903103087837059E-6	0.0	4.9515515439185296E-6	1.5349809786147442E-4
17	3.3175395344254145E-4	9.903103087837059E-6	4.9515515439185296E-6	4.9515515439185296E-6	1.6340120094931147E-4
18	3.3670550498646E-4	1.4854654631755588E-5	4.9515515439185296E-6	9.903103087837059E-6	1.6835275249323E-4
19	3.5156015961821557E-4	1.4854654631755588E-5	4.9515515439185296E-6	9.903103087837059E-6	1.832074071249856E-4
20	3.8126946888172674E-4	1.4854654631755588E-5	4.9515515439185296E-6	9.903103087837059E-6	1.9806206175674118E-4
21	4.060272266013194E-4	1.9806206175674118E-5	4.9515515439185296E-6	9.903103087837059E-6	2.0796516484457823E-4
22	4.258334327769935E-4	1.9806206175674118E-5	4.9515515439185296E-6	9.903103087837059E-6	2.0796516484457823E-4
23	4.5059119049658614E-4	1.9806206175674118E-5	4.9515515439185296E-6	9.903103087837059E-6	2.178682679324153E-4
24	5.0505825747969E-4	1.9806206175674118E-5	4.9515515439185296E-6	9.903103087837059E-6	2.178682679324153E-4
25	5.298160151992826E-4	1.9806206175674118E-5	4.9515515439185296E-6	9.903103087837059E-6	2.2777137102025235E-4
26	5.941861852702234E-4	1.9806206175674118E-5	4.9515515439185296E-6	9.903103087837059E-6	2.3272292256417088E-4
27	6.288470460776532E-4	3.46608608074297E-5	4.9515515439185296E-6	9.903103087837059E-6	2.4757757719592644E-4
28	6.536048037972459E-4	3.46608608074297E-5	4.9515515439185296E-6	9.903103087837059E-6	2.4757757719592644E-4
29	6.932172161485941E-4	3.46608608074297E-5	4.9515515439185296E-6	9.903103087837059E-6	2.4757757719592644E-4
30	7.377811800438609E-4	3.46608608074297E-5	4.9515515439185296E-6	9.903103087837059E-6	2.6243223182768205E-4
31	7.872966954830461E-4	3.46608608074297E-5	4.9515515439185296E-6	9.903103087837059E-6	2.772868864594376E-4
32	8.318606593783129E-4	3.46608608074297E-5	4.9515515439185296E-6	9.903103087837059E-6	3.3175395344254145E-4
33	9.903103087837057E-4	3.46608608074297E-5	4.9515515439185296E-6	9.903103087837059E-6	3.3670550498646E-4
34	0.0010843897881181578	3.46608608074297E-5	4.9515515439185296E-6	9.903103087837059E-6	3.3670550498646E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	15625	0.0	21.2992	1
AGCAGTG	24175	0.0	13.840745	2
TCAACGC	25050	0.0	13.453094	12
AGTGGTA	25355	0.0	13.401696	5
CAACGCA	25200	0.0	13.380953	13
AACGCAG	25745	0.0	13.152069	14
ACGCAGA	26070	0.0	12.980438	15
GTGGTAT	26255	0.0	12.942296	6
ATCAACG	26170	0.0	12.930837	11
GGTATCA	26755	0.0	12.633152	8
GCAGTGG	26990	0.0	12.619489	3
GTATCAA	26960	0.0	12.589021	9
TATCAAC	27300	0.0	12.388278	10
CGCAGAG	27670	0.0	12.302132	16
AGAGTAC	27490	0.0	12.149873	19
TGGTATC	27900	0.0	12.121863	7
CAGTGGT	28860	0.0	11.912682	4
GAGTACT	10745	0.0	11.186599	20
CAGAGTA	30630	0.0	11.074111	18
GCAGAGT	31390	0.0	10.907932	17
>>END_MODULE