FastQCFastQC Report
Thu 26 May 2016
SRR805212_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805212_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7081132
Sequences flagged as poor quality0
Sequence length46
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT715951.011067157059069No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT155210.21918811850986536No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT202700.035.500741
AGCAGTG258600.027.9891722
GCAGTGG264750.027.4674223
AGTGGTA270800.026.9867085
CAACGCA273450.026.97385413
TCAACGC274600.026.8900212
GGTATCA275000.026.7272728
GTGGTAT273750.026.7251156
AACGCAG276200.026.70528414
ACGCAGA276450.026.65943315
TGGTATC275900.026.53867
GTATCAA278650.026.3987089
ATCAACG280600.026.37205911
CAGTGGT277800.026.2994944
CGCAGAG280550.026.28408216
GAGTACT118600.026.2394620
TATCAAC280850.026.18479510
AGAGTAC279850.025.86385519
CAGAGTA287050.025.326618
GCAGAGT292250.025.02651817
Produced by FastQC (version 0.11.5)