FastQCFastQC Report
Thu 26 May 2016
SRR805211_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805211_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18505719
Sequences flagged as poor quality0
Sequence length46
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT4081672.2056262715326005No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGC245610.1327211333966543No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCC231640.12517211571190506No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1295850.037.80531
AGCAGTG1374000.035.68852
GTGGTAT1375800.035.4193956
TCAACGC1384300.035.40273312
AGTGGTA1377450.035.4001925
CAACGCA1387550.035.33854713
ATCAACG1391800.035.2133911
AACGCAG1396050.035.13914514
GGTATCA1389950.035.110628
GCAGTGG1397650.035.0931933
ACGCAGA1399100.035.06968715
GTATCAA1392400.035.0531469
TGGTATC1393750.034.9847537
TATCAAC1402900.034.83783310
CGCAGAG1414250.034.69259316
CAGTGGT1409550.034.609634
AGAGTAC1401650.034.60350419
GAGTACT525700.034.32375720
GTACTTT522900.034.17479322
CAGAGTA1426500.034.13950318
Produced by FastQC (version 0.11.5)