##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805211_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	18505719
Sequences flagged as poor quality	0
Sequence length	46
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.15790556422044	37.0	35.0	37.0	35.0	37.0
2	36.19022940962196	37.0	37.0	37.0	35.0	37.0
3	37.98197946267313	39.0	39.0	39.0	35.0	39.0
4	37.95840928958232	39.0	38.0	39.0	35.0	39.0
5	37.93350790639369	39.0	38.0	39.0	35.0	39.0
6	37.90925453909681	39.0	38.0	39.0	35.0	39.0
7	37.83109297185373	39.0	38.0	39.0	35.0	39.0
8	38.796252282875365	40.0	39.0	40.0	36.0	40.0
9	38.81446065402809	40.0	39.0	40.0	36.0	40.0
10	38.62962411782001	40.0	39.0	40.0	36.0	40.0
11	38.669674979934584	40.0	39.0	40.0	36.0	40.0
12	38.71197044546067	40.0	39.0	40.0	36.0	40.0
13	38.784552656397736	40.0	39.0	40.0	36.0	40.0
14	38.73602506338716	40.0	39.0	40.0	36.0	40.0
15	38.67179562166701	40.0	39.0	40.0	36.0	40.0
16	38.6502208857705	40.0	39.0	40.0	36.0	40.0
17	38.53980880180878	40.0	39.0	40.0	36.0	40.0
18	38.59355072883145	40.0	39.0	40.0	36.0	40.0
19	38.57524006497667	40.0	39.0	40.0	36.0	40.0
20	38.44043762903781	40.0	38.0	40.0	35.0	40.0
21	38.32921817304153	40.0	38.0	40.0	35.0	40.0
22	38.35290031151992	40.0	38.0	40.0	35.0	40.0
23	38.14543947198161	40.0	38.0	40.0	35.0	40.0
24	38.09534571447886	40.0	38.0	40.0	34.0	40.0
25	38.093445815317956	40.0	38.0	40.0	34.0	40.0
26	38.061473915171845	40.0	38.0	40.0	34.0	40.0
27	37.973544988984216	40.0	38.0	40.0	34.0	40.0
28	37.88664887865205	40.0	38.0	40.0	34.0	40.0
29	37.80844056910191	40.0	38.0	40.0	34.0	40.0
30	37.795170238994764	40.0	38.0	40.0	34.0	40.0
31	37.63960919324453	40.0	38.0	40.0	33.0	40.0
32	37.601427212852414	40.0	37.0	40.0	33.0	40.0
33	37.49148530786618	40.0	37.0	40.0	33.0	40.0
34	37.382535906872896	39.0	37.0	40.0	33.0	40.0
35	37.2893908094033	39.0	37.0	40.0	33.0	40.0
36	37.156184690797474	39.0	37.0	40.0	33.0	40.0
37	37.04391982824337	38.0	36.0	40.0	33.0	40.0
38	36.85975692163055	38.0	36.0	40.0	32.0	40.0
39	36.790406036101594	38.0	36.0	40.0	32.0	40.0
40	36.63460182227991	38.0	35.0	40.0	32.0	40.0
41	36.42128214526547	38.0	35.0	40.0	31.0	40.0
42	36.28468291342801	38.0	35.0	40.0	31.0	40.0
43	36.112435782689666	38.0	35.0	40.0	31.0	40.0
44	36.01555232736431	38.0	35.0	40.0	31.0	40.0
45	35.95434535669757	38.0	35.0	40.0	31.0	40.0
46	36.591488609548215	38.0	35.0	40.0	31.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	5.0
10	5.0
11	12.0
12	25.0
13	51.0
14	178.0
15	543.0
16	1130.0
17	1782.0
18	2674.0
19	3913.0
20	6012.0
21	9740.0
22	15608.0
23	24145.0
24	35714.0
25	51072.0
26	69731.0
27	92615.0
28	120577.0
29	152941.0
30	192974.0
31	241270.0
32	301687.0
33	379943.0
34	495039.0
35	680273.0
36	1013926.0
37	1838107.0
38	5148190.0
39	7625837.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	15.624375362016465	35.20379835012085	24.72239527683307	24.449431011029617
2	26.96298371330506	35.80530969912598	18.96878473081754	18.26292185675142
3	30.670080962539203	25.327051599562278	21.226394932290933	22.77647250560759
4	21.936656446582813	24.70232040160126	22.481201622049916	30.879821529766016
5	22.63870968752957	31.21156762404098	24.023454587200852	22.1262681012286
6	29.470932742467344	24.10747185775381	23.331425274532698	23.090170125246146
7	23.321741781554124	23.91719013997781	30.339026546334136	22.42204153213393
8	28.520334713825495	24.817003867831346	24.393043037128145	22.269618381215018
9	28.575528462309407	24.322924172792206	24.85805063829187	22.243496726606516
10	22.054225507260757	24.971972177898085	30.6607487123305	22.313053602510664
11	22.042645303324882	31.456897189458026	24.377550529109406	22.122906978107686
12	21.94110912415778	24.769407770646467	30.700449952795672	22.589033152400077
13	21.9821288759437	25.03758432730984	24.103597379815398	28.876689416931058
14	22.455290713103338	31.540125514712507	23.955481005628585	22.04910276655557
15	22.201790700485617	31.123032831094	23.965531952581795	22.70964451583859
16	22.545716813272698	24.578812636245043	24.045328906161387	28.83014164432087
17	29.185048146467587	24.38531029245608	24.025902479120102	22.403739081956232
18	22.339072586155666	24.707794385076312	24.005930274851792	28.947202753916233
19	22.728411687219502	31.01845434916633	23.979965328556	22.27316863505817
20	29.091255519442395	24.667487926299973	24.153970996749706	22.087285557507926
21	22.61175585774322	30.9786882638821	24.113410562432076	22.2961453159426
22	28.816918704968984	24.32529641242256	24.46390221314827	22.393882669460183
23	22.448373932404355	24.646748391672865	30.753082330926993	22.15179534499578
24	22.406894863150143	30.736865722428835	24.215470903886523	22.6407685105345
25	22.353873416104502	24.53347530025718	24.236199631043785	28.876451652594533
26	22.64831212448433	28.339514935896304	26.865813751954192	22.146359187665176
27	22.83078544529937	24.209191763908226	30.66033262474157	22.29969016605083
28	26.721415147393085	24.305048617673272	26.6430555873025	22.330480647631145
29	26.83307252206737	24.369893436726237	26.56538230154689	22.231651739659508
30	26.677709739351386	24.25026014930844	26.5846358090707	22.487394302269475
31	25.151684190168456	24.895703863221957	26.94782623685143	23.004785709758156
32	24.232287326960925	25.59982133090857	27.198365002732398	22.969526339398108
33	23.982580736257802	25.254301116319773	27.356872759172447	23.406245388249978
34	23.646376560672948	25.203484393121933	27.588109383915317	23.562029662289806
35	23.604459788890125	25.33292545942149	27.666587826174165	23.396026925514217
36	23.642210281048794	25.256954350166023	27.635413679414455	23.46542168937073
37	23.83402125580746	25.20286296360601	27.61408513768095	23.34903064290558
38	23.98691993539943	25.240159542031304	27.617802907306654	23.15511761526261
39	23.94400347265621	25.178135472607146	27.567996682539057	23.309864372197588
40	23.669012806257353	25.380364848293656	27.465082550967086	23.48553979448191
41	23.87224187290426	25.56477270621044	27.39234827892934	23.170637141955954
42	23.828028513779984	25.41585657925531	27.469070507338838	23.287044399625866
43	23.765604567971664	25.31602257658835	27.667166025810726	23.25120682962926
44	23.902799993882972	25.25865112293124	27.465114973376608	23.37343390980918
45	23.883762635756007	25.037649172128894	27.434043497580397	23.6445446945347
46	23.73418184940558	25.165744708433106	27.361601027228392	23.738472414932918
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	5372.0
1	3397.0
2	1422.0
3	1226.5
4	1031.0
5	1162.5
6	1294.0
7	1294.0
8	1856.0
9	2418.0
10	3664.5
11	4911.0
12	8236.0
13	11561.0
14	15238.5
15	18916.0
16	25894.0
17	32872.0
18	47647.5
19	62423.0
20	62423.0
21	80440.5
22	98458.0
23	128874.0
24	159290.0
25	403061.0
26	646832.0
27	475197.5
28	303563.0
29	328379.5
30	353196.0
31	353196.0
32	401560.5
33	449925.0
34	507417.0
35	564909.0
36	634968.0
37	705027.0
38	781601.5
39	858176.0
40	940293.5
41	1022411.0
42	1111443.5
43	1200476.0
44	1200476.0
45	1295615.5
46	1390755.0
47	1451220.0
48	1511685.0
49	1543361.5
50	1575038.0
51	1565870.0
52	1556702.0
53	1503420.0
54	1450138.0
55	1379331.0
56	1308524.0
57	1308524.0
58	1168180.0
59	1027836.0
60	907786.0
61	787736.0
62	674230.5
63	560725.0
64	465102.5
65	369480.0
66	300267.0
67	231054.0
68	177278.5
69	123503.0
70	123503.0
71	91250.5
72	58998.0
73	42910.0
74	26822.0
75	19676.5
76	12531.0
77	8935.0
78	5339.0
79	3872.0
80	2405.0
81	2405.0
82	1810.5
83	1216.0
84	823.5
85	431.0
86	343.0
87	255.0
88	146.5
89	38.0
90	24.5
91	11.0
92	10.0
93	9.0
94	9.0
95	6.5
96	4.0
97	2.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
46	1.8505719E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.0398583811774
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.56194705890343	26.90653022491647
2	16.464384841620884	13.5139204446665
3	6.7856204578595865	8.354427078569328
4	3.2292431714332896	5.3011072973602555
5	1.8626152260453295	3.822073254776253
6	1.1442753639663077	2.81765393317485
7	0.786002516489772	2.258020237479251
8	0.5771936483478405	1.895035646936839
9	0.44173880106142555	1.6316008053328799
>10	2.9514785839928552	22.71299291798701
>50	0.15164030679910365	4.124238611075381
>100	0.04082754244872412	2.962813124184886
>500	0.0025321336171735664	0.6635648713175113
>1k	4.082373419140893E-4	0.32767609294531375
>5k	2.6317163511554074E-5	0.06575360615439559
>10k+	6.579290877888516E-5	2.642591853122757
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	408167	2.2056262715326005	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGC	24561	0.1327211333966543	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCC	23164	0.12517211571190506	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.242240952648206E-4	0.0	0.0	0.0	1.621120476324103E-5
2	5.349697571869539E-4	0.0	0.0	0.0	1.621120476324103E-5
3	5.457772270291146E-4	0.0	0.0	0.0	4.8633614289723086E-5
4	5.457772270291146E-4	0.0	0.0	5.403734921080343E-6	6.484481905296411E-5
5	5.781996365555967E-4	0.0	0.0	5.403734921080343E-6	9.186349365836582E-5
6	6.052183111609984E-4	0.0	0.0	5.403734921080343E-6	1.0267096350052652E-4
7	6.106220460820787E-4	0.0	0.0	1.0807469842160686E-5	1.0267096350052652E-4
8	6.214295159242394E-4	0.0	0.0	1.0807469842160686E-5	1.0807469842160685E-4
9	6.268332508453198E-4	0.0	0.0	1.0807469842160686E-5	1.0807469842160685E-4
10	6.268332508453198E-4	0.0	0.0	1.0807469842160686E-5	1.242859031848479E-4
11	6.322369857664001E-4	0.0	0.0	1.0807469842160686E-5	1.4049710794808892E-4
12	6.430444556085608E-4	0.0	0.0	1.0807469842160686E-5	1.513045777902496E-4
13	6.646593952928821E-4	0.0	0.0	1.0807469842160686E-5	1.621120476324103E-4
14	7.132930095826053E-4	5.403734921080343E-6	0.0	1.0807469842160686E-5	2.0534192700105304E-4
15	7.403116841880069E-4	5.403734921080343E-6	0.0	1.0807469842160686E-5	2.1074566192213337E-4
16	7.997527683198907E-4	5.403734921080343E-6	0.0	1.621120476324103E-5	2.3236060160645473E-4
17	8.483863826096138E-4	5.403734921080343E-6	0.0	1.621120476324103E-5	2.377643365275351E-4
18	8.754050572150155E-4	5.403734921080343E-6	0.0	2.7018674605401713E-5	2.539755412907761E-4
19	9.078274667414976E-4	5.403734921080343E-6	0.0	3.78261444475624E-5	2.7559048097509746E-4
20	9.564610810312207E-4	5.403734921080343E-6	0.0	3.78261444475624E-5	3.242240952648206E-4
21	0.0010159021651631045	5.403734921080343E-6	0.0	3.78261444475624E-5	3.296278301859009E-4
22	0.0010267096350052652	5.403734921080343E-6	0.0	4.8633614289723086E-5	3.4583903494914196E-4
23	0.0011077656588214702	5.403734921080343E-6	0.0	4.8633614289723086E-5	3.6205023971238296E-4
24	0.0011726104778744344	5.403734921080343E-6	0.0	5.4037349210803427E-5	3.78261444475624E-4
25	0.0012374552969273984	5.403734921080343E-6	0.0	6.484481905296411E-5	3.78261444475624E-4
26	0.001291492646138202	5.403734921080343E-6	0.0	7.56522888951248E-5	3.8366517939670435E-4
27	0.0013833561397965679	5.403734921080343E-6	0.0	7.56522888951248E-5	3.890689143177847E-4
28	0.0014590084286916925	5.403734921080343E-6	0.0	8.645975873728549E-5	3.94472649238865E-4
29	0.0015184495128235764	5.403734921080343E-6	0.0	8.645975873728549E-5	3.9987638415994536E-4
30	0.0016427354160084243	5.403734921080343E-6	0.0	8.645975873728549E-5	4.052801190810257E-4
31	0.0018210586684040755	5.403734921080343E-6	0.0	8.645975873728549E-5	4.106838540021061E-4
32	0.002031804330326209	5.403734921080343E-6	0.0	9.726722857944617E-5	4.106838540021061E-4
33	0.002253357462090503	5.403734921080343E-6	0.0	1.0267096350052652E-4	4.4850999844966847E-4
34	0.0024208732446439935	5.403734921080343E-6	0.0	1.0267096350052652E-4	4.539137333707488E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	129585	0.0	37.8053	1
AGCAGTG	137400	0.0	35.6885	2
GTGGTAT	137580	0.0	35.419395	6
TCAACGC	138430	0.0	35.402733	12
AGTGGTA	137745	0.0	35.400192	5
CAACGCA	138755	0.0	35.338547	13
ATCAACG	139180	0.0	35.21339	11
AACGCAG	139605	0.0	35.139145	14
GGTATCA	138995	0.0	35.11062	8
GCAGTGG	139765	0.0	35.093193	3
ACGCAGA	139910	0.0	35.069687	15
GTATCAA	139240	0.0	35.053146	9
TGGTATC	139375	0.0	34.984753	7
TATCAAC	140290	0.0	34.837833	10
CGCAGAG	141425	0.0	34.692593	16
CAGTGGT	140955	0.0	34.60963	4
AGAGTAC	140165	0.0	34.603504	19
GAGTACT	52570	0.0	34.323757	20
GTACTTT	52290	0.0	34.174793	22
CAGAGTA	142650	0.0	34.139503	18
>>END_MODULE