FastQCFastQC Report
Thu 26 May 2016
SRR805210_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805210_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20665156
Sequences flagged as poor quality0
Sequence length53
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT7334533.549225565972016No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCC665600.32208805972720456No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGG615240.29771853645818114No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCAGTG433100.20957983573896077No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTA415860.20123729044194005No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTG371130.17959215986562113No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTC288470.13959246182317714No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGGCTGGTGAGATGGCTCAGT220600.10674973854540465No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT4700450.046.0760881
AGCAGTG4780850.045.3061372
GTGGTAT4780900.045.2132536
AGTGGTA4787200.045.1557165
TCAACGC4802200.045.0949212
GTATCAA4798150.045.0791139
TGGTATC4793100.045.078567
CAACGCA4806050.045.07151413
GGTATCA4801850.045.0541658
TATCAAC4807700.045.01009810
ATCAACG4813650.045.0003511
AACGCAG4817600.044.9634614
ACGCAGA4815350.044.9551815
GCAGTGG4818100.044.919283
CAGTGGT4824750.044.7833334
AGAGTAC4781000.044.75764519
CGCAGAG4839650.044.7576216
CAGAGTA4814850.044.59282318
GCAGAGT4860350.044.3789217
GTACATG3437850.044.32178522