##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805210_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	20665156
Sequences flagged as poor quality	0
Sequence length	53
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.97721885090052	37.0	35.0	37.0	35.0	37.0
2	36.04363359270068	37.0	35.0	37.0	35.0	37.0
3	37.95743927604514	39.0	38.0	39.0	35.0	39.0
4	37.86662621854875	39.0	38.0	39.0	35.0	39.0
5	37.86825398269435	39.0	38.0	39.0	35.0	39.0
6	37.83863097863863	39.0	38.0	39.0	35.0	39.0
7	37.66570409630588	39.0	37.0	39.0	35.0	39.0
8	38.68917955422161	40.0	39.0	40.0	36.0	40.0
9	38.656682872367384	40.0	39.0	40.0	36.0	40.0
10	38.38998776491211	40.0	38.0	40.0	36.0	40.0
11	38.360938286650246	40.0	38.0	40.0	36.0	40.0
12	38.55144790583724	40.0	38.0	40.0	36.0	40.0
13	38.64575796088837	40.0	39.0	40.0	36.0	40.0
14	38.55014489123624	40.0	38.0	40.0	36.0	40.0
15	38.488955950780145	40.0	38.0	40.0	36.0	40.0
16	38.445777713945155	40.0	38.0	40.0	36.0	40.0
17	38.10463777771627	40.0	38.0	40.0	35.0	40.0
18	38.204956691350404	40.0	38.0	40.0	35.0	40.0
19	38.14487323492743	40.0	38.0	40.0	35.0	40.0
20	37.76073139733376	40.0	37.0	40.0	34.0	40.0
21	37.33678986018784	39.0	37.0	40.0	33.0	40.0
22	37.65863640226089	39.0	37.0	40.0	34.0	40.0
23	37.079795768297124	38.0	36.0	40.0	33.0	40.0
24	37.115778221079	38.0	36.0	40.0	33.0	40.0
25	36.808379138294434	38.0	36.0	40.0	32.0	40.0
26	36.98182805878649	38.0	36.0	40.0	33.0	40.0
27	36.876648741485425	38.0	36.0	40.0	32.0	40.0
28	36.56064290054234	38.0	36.0	40.0	32.0	40.0
29	36.092468984990965	38.0	35.0	39.0	31.0	40.0
30	35.89831056683047	38.0	35.0	39.0	30.0	40.0
31	35.61839436392351	38.0	34.0	39.0	30.0	40.0
32	35.33685359065279	37.0	34.0	39.0	30.0	40.0
33	34.93071317729225	37.0	33.0	39.0	29.0	40.0
34	34.69170399681474	37.0	33.0	38.0	28.0	40.0
35	34.47629415427592	36.0	33.0	38.0	27.0	40.0
36	34.270560599687705	36.0	33.0	38.0	27.0	39.0
37	33.872779619955445	36.0	32.0	38.0	27.0	39.0
38	33.50674923528281	35.0	31.0	38.0	26.0	39.0
39	33.35639779346452	35.0	31.0	38.0	26.0	39.0
40	33.20557657537161	35.0	31.0	38.0	26.0	39.0
41	32.882439164746685	35.0	31.0	37.0	25.0	38.0
42	32.569888753803745	34.0	31.0	37.0	24.0	38.0
43	32.14270683463507	34.0	30.0	37.0	24.0	38.0
44	31.853302776906208	34.0	29.0	37.0	24.0	38.0
45	31.740184153461023	33.0	29.0	36.0	24.0	38.0
46	31.541547375688815	33.0	29.0	36.0	23.0	38.0
47	31.310868836412364	33.0	29.0	36.0	23.0	38.0
48	30.968393028342007	33.0	28.0	36.0	23.0	38.0
49	30.657359325039696	32.0	28.0	35.0	22.0	38.0
50	30.21171091086852	32.0	27.0	35.0	21.0	37.0
51	29.733869998368267	31.0	27.0	35.0	20.0	37.0
52	29.336304453738457	31.0	26.0	34.0	19.0	37.0
53	30.90843635538004	33.0	29.0	35.0	22.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	10.0
12	42.0
13	179.0
14	599.0
15	1399.0
16	2612.0
17	3851.0
18	5777.0
19	9943.0
20	17661.0
21	29570.0
22	45446.0
23	65317.0
24	90066.0
25	121280.0
26	158425.0
27	201547.0
28	254017.0
29	315191.0
30	391225.0
31	497920.0
32	661703.0
33	944028.0
34	1521932.0
35	2978357.0
36	6011400.0
37	5575784.0
38	756311.0
39	3564.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	12.973712852687877	43.32823328311676	26.672428700755997	17.02562516343937
2	22.065320968300455	48.2332047239324	11.995951058874175	17.70552324889297
3	44.242608185488656	22.036030117556336	15.48651265928019	18.23484903767482
4	20.414634179388724	19.38752361704891	17.18063972030988	43.01720248325249
5	18.90802566406951	42.174905430184026	19.729485710149007	19.187583195597462
6	41.95092938083796	20.167043500663627	18.259992811087418	19.62203430741099
7	19.949745358805902	19.421111556089873	41.744369120658945	18.884773964445273
8	41.42770081193677	21.14065821714581	19.241146788342657	18.190494182574763
9	41.11855240773406	20.8081903664313	19.533339114401073	18.539918111433565
10	18.513032275197922	20.80624022388217	42.121453135896964	18.55927436502294
11	18.48150093810083	42.83949755811183	19.993684054453787	18.685317449333557
12	18.364235914793	20.698145225712306	42.193143860128615	18.74447499936608
13	18.307638229297666	20.787382393822725	20.147851775229764	40.75712760164985
14	18.711530655756967	42.93897418437103	19.877749773580224	18.471745386291786
15	18.595712512404937	42.683040960348904	19.904451725406766	18.816794801839386
16	18.599036948958915	20.33221041254177	20.195434285615846	40.87331835288347
17	40.89900410139657	20.32170964496953	20.10520994857237	18.674076305061522
18	18.63893018760662	20.456196894908512	20.11583169272954	40.78904122475534
19	18.7233089360661	42.65142735917406	20.153798984145098	18.47146472061474
20	41.08874861626982	20.387617688441356	20.15729762698138	18.366336068307447
21	18.730252992041287	42.50078247655135	20.223152440755833	18.545812090651527
22	40.74077640643022	20.259077647417712	20.435456669187495	18.564689276964568
23	18.732846730022263	20.40090575653046	42.17784758072961	18.68839993271766
24	18.685322288396954	42.32720043342523	20.11358636731317	18.873890910864645
25	18.635049258761946	20.410046747288042	20.311421796186778	40.643482197763234
26	18.996866028981344	36.0396263159107	26.34711782480616	18.616389830301788
27	19.183963576176247	20.26724114736903	41.98301237116236	18.565782905292366
28	34.86576147791964	20.195560101264178	26.364340051437306	18.57433836937887
29	34.82259703241534	20.29600454020284	26.2532303167709	18.628168110610925
30	34.53762942800916	20.352457053796254	26.264858586114713	18.845054932079876
31	28.32643024809491	22.687063189844782	27.89819249368357	21.08831406837674
32	24.500168302624957	25.303568964105573	28.848855532472147	21.34740720079732
33	23.849062644385555	24.132549495392148	29.68563121420424	22.33275664601806
34	23.088086051709457	23.49436897548705	30.468615867211458	22.948929105592043
35	22.67367350142433	23.92181312350122	30.512719091014844	22.891794284059603
36	22.567359278584686	24.18592436466485	30.614416847373427	22.63229950937704
37	23.55394752403514	23.676554873333643	30.50693641025502	22.26256119237619
38	23.838692531525048	23.842505713482154	30.14596163706676	22.17284011792604
39	23.854400131312826	23.84564626562703	30.111943021383436	22.18801058167671
40	23.049755830539098	24.18995046541144	29.873140081787913	22.88715362226155
41	23.38151717799759	24.886112642943516	29.494822105383573	22.23754807367532
42	23.503480931864246	24.860025252168434	29.708132859001886	21.928360956965435
43	23.474625596825884	24.108068673664985	30.545426320517493	21.87187940899164
44	23.427551188096523	24.016489398870252	29.919483791944273	22.636475621088948
45	23.523369482427327	23.5536620192947	29.900911466625267	23.0220570316527
46	23.263294020137085	23.633414623146322	29.73490255771599	23.3683887990006
47	23.328408457211744	23.80090428545519	29.696775577208324	23.173911680124746
48	23.27390124710406	23.885045919808203	29.730842583525625	23.110210249562112
49	22.618890464703	24.76386822339981	29.602704184763954	23.014537127133227
50	23.620683047347914	24.37777871117934	29.231664159709226	22.769874081763525
51	23.279848456019398	24.194963735091086	29.392858200538143	23.13232960835137
52	23.79126487116768	24.070091704122632	28.979311842601142	23.15933158210855
53	24.41893494537375	24.385090535972726	28.162816675567313	23.033157843086208
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2380.0
1	1513.5
2	647.0
3	535.0
4	423.0
5	425.5
6	428.0
7	503.5
8	760.5
9	942.0
10	1543.0
11	2144.0
12	3278.5
13	4413.0
14	5866.0
15	7319.0
16	10800.0
17	14281.0
18	20156.5
19	26032.0
20	39494.5
21	52957.0
22	485917.0
23	918877.0
24	624424.0
25	293888.5
26	257806.0
27	259293.5
28	260781.0
29	274932.5
30	289084.0
31	309592.0
32	330100.0
33	370195.0
34	410290.0
35	462173.0
36	514056.0
37	576540.0
38	639024.0
39	709017.5
40	779011.0
41	860974.5
42	1027793.5
43	1112649.0
44	1214406.5
45	1316164.0
46	1394302.5
47	1472441.0
48	1557818.5
49	1643196.0
50	1637244.5
51	1631293.0
52	1602506.0
53	1573719.0
54	1534453.5
55	1495188.0
56	1446365.5
57	1397543.0
58	1216402.0
59	894164.0
60	753067.0
61	648325.5
62	543584.0
63	461794.0
64	380004.0
65	308926.0
66	237848.0
67	189473.0
68	141098.0
69	109839.5
70	78581.0
71	57206.5
72	35832.0
73	26482.5
74	17133.0
75	13105.5
76	6579.5
77	4081.0
78	2811.0
79	1541.0
80	1208.0
81	875.0
82	604.0
83	333.0
84	217.0
85	101.0
86	66.5
87	32.0
88	24.0
89	16.0
90	12.5
91	9.0
92	5.0
93	2.5
94	4.0
95	2.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	2.0665156E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.36644055858586
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.84857305559385	30.13486167082022
2	13.907367733908035	11.505966013822032
3	4.794317031409992	5.949714914965118
4	2.181557356487349	3.609730508491185
5	1.2392435145995673	2.5631546592148013
6	0.8232074414401361	2.0431897016231364
7	0.5643880314798273	1.6342706769331292
8	0.42614266991176625	1.4102404339505723
9	0.32676262741937234	1.2165306123519724
>10	2.6653069359146913	23.658441174410335
>50	0.17081505967422447	4.660844415623082
>100	0.04649935564253375	3.497738493634777
>500	0.0037642624360717893	1.0411112259397763
>1k	0.0018161267763469162	1.4559823318467542
>5k	1.0745878785272108E-4	0.33139367171073975
>10k+	1.3133851848665673E-4	5.286829494662246
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	733453	3.549225565972016	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCC	66560	0.32208805972720456	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGG	61524	0.29771853645818114	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCAGTG	43310	0.20957983573896077	No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTA	41586	0.20123729044194005	No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTG	37113	0.17959215986562113	No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTC	28847	0.13959246182317714	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGGCTGGTGAGATGGCTCAGT	22060	0.10674973854540465	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.113203887742246E-4	0.0	0.0	9.678126794687637E-6	9.678126794687637E-6
2	4.935844665290695E-4	0.0	0.0	9.678126794687637E-6	1.4517190192031456E-5
3	5.516532272971953E-4	0.0	0.0	9.678126794687637E-6	3.387344378140673E-5
4	5.758485442839144E-4	0.0	0.0	1.4517190192031456E-5	3.387344378140673E-5
5	5.903657344759459E-4	0.0	0.0	1.4517190192031456E-5	4.839063397343819E-5
6	6.097219880653212E-4	0.0	0.0	1.4517190192031456E-5	4.839063397343819E-5
7	6.14561051462665E-4	0.0	0.0	2.4195316986719094E-5	5.3229697370782006E-5
8	6.242391782573526E-4	0.0	0.0	2.9034380384062912E-5	5.3229697370782006E-5
9	6.290782416546964E-4	0.0	0.0	2.9034380384062912E-5	7.258595096015728E-5
10	6.290782416546964E-4	0.0	0.0	3.871250717875055E-5	7.74250143575011E-5
11	6.339173050520403E-4	0.0	0.0	4.355157057609437E-5	9.678126794687638E-5
12	6.435954318467279E-4	0.0	0.0	4.839063397343819E-5	1.0645939474156401E-4
13	6.581126220387593E-4	0.0	0.0	5.3229697370782006E-5	1.0645939474156401E-4
14	6.67790748833447E-4	0.0	0.0	5.3229697370782006E-5	1.4517190192031457E-4
15	6.726298122307908E-4	0.0	0.0	6.290782416546965E-5	1.500109653176584E-4
16	6.774688756281346E-4	0.0	0.0	6.774688756281346E-5	1.6452815550968984E-4
17	7.065032560121976E-4	0.0	0.0	6.774688756281346E-5	1.9356253589375275E-4
18	7.258595096015728E-4	0.0	0.0	8.710314115218874E-5	2.1775785288047185E-4
19	7.403766997936043E-4	0.0	0.0	9.194220454953255E-5	2.322750430725033E-4
20	7.645720167803234E-4	0.0	0.0	9.678126794687638E-5	2.564703600592224E-4
21	8.371579677404807E-4	0.0	0.0	9.678126794687638E-5	2.564703600592224E-4
22	8.613532847271997E-4	0.0	0.0	1.0645939474156401E-4	2.613094234565662E-4
23	9.387782990847008E-4	0.0	0.0	1.1129845813890784E-4	2.855047404432853E-4
24	0.0010016861232501704	0.0	0.0	1.1129845813890784E-4	2.9518286723797296E-4
25	0.00106459394741564	0.0	0.0	1.1129845813890784E-4	3.000219306353168E-4
26	0.001122662708183766	0.0	0.0	1.1613752153625165E-4	3.048609940326606E-4
27	0.0011420189617731412	0.0	0.0	1.1613752153625165E-4	3.097000574300044E-4
28	0.0011904095957465794	0.0	0.0	1.2097658493359547E-4	3.4357350121141115E-4
29	0.0012871908636934559	0.0	0.0	1.3549377512562692E-4	3.5809069140344256E-4
30	0.0013694549414483008	0.0	0.0	1.4033283852297074E-4	3.677688181981302E-4
31	0.0015339830969579905	0.0	0.0	1.4517190192031457E-4	3.7260788159547404E-4
32	0.0016694768720836174	0.0	0.0	1.59689092112346E-4	3.7260788159547404E-4
33	0.0018630394079773701	0.0	0.0	1.838844090990651E-4	3.871250717875055E-4
34	0.001974337866116278	4.839063397343818E-6	0.0	1.9356253589375275E-4	3.9196413518484933E-4
35	0.0021146707046392488	4.839063397343818E-6	0.0	1.9840159929109658E-4	3.9196413518484933E-4
36	0.0022501644797648756	4.839063397343818E-6	0.0	2.080797260857842E-4	4.113203887742246E-4
37	0.0023953363816851905	4.839063397343818E-6	0.0	2.1291878948312803E-4	4.209985155689122E-4
38	0.0025985770443736306	4.839063397343818E-6	0.0	2.2259691627781567E-4	4.2583757896625605E-4
39	0.002806656770459415	4.839063397343818E-6	0.0	2.4195316986719094E-4	4.306766423635999E-4
40	0.003097000574300044	4.839063397343818E-6	0.0	2.4679223326453477E-4	4.403547691582875E-4
41	0.003416378758524736	4.839063397343818E-6	0.0	2.564703600592224E-4	4.5487195935031894E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	470045	0.0	46.076088	1
AGCAGTG	478085	0.0	45.306137	2
GTGGTAT	478090	0.0	45.213253	6
AGTGGTA	478720	0.0	45.155716	5
TCAACGC	480220	0.0	45.09492	12
GTATCAA	479815	0.0	45.079113	9
TGGTATC	479310	0.0	45.07856	7
CAACGCA	480605	0.0	45.071514	13
GGTATCA	480185	0.0	45.054165	8
TATCAAC	480770	0.0	45.010098	10
ATCAACG	481365	0.0	45.00035	11
AACGCAG	481760	0.0	44.96346	14
ACGCAGA	481535	0.0	44.95518	15
GCAGTGG	481810	0.0	44.91928	3
CAGTGGT	482475	0.0	44.783333	4
AGAGTAC	478100	0.0	44.757645	19
CGCAGAG	483965	0.0	44.75762	16
CAGAGTA	481485	0.0	44.592823	18
GCAGAGT	486035	0.0	44.37892	17
GTACATG	343785	0.0	44.321785	22
>>END_MODULE