FastQCFastQC Report
Thu 26 May 2016
SRR805209_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805209_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20128486
Sequences flagged as poor quality0
Sequence length53
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3291881.63543348466447No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGG331590.16473668213297313No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCAGTG260530.12943348049127987No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1693100.044.416961
TCAACGC1754050.042.81326312
CAACGCA1757200.042.75256313
GTGGTAT1759650.042.6396186
GTATCAA1765950.042.5101249
GGTATCA1767100.042.5090568
ACGCAGA1766900.042.4965815
AGTGGTA1767400.042.4832275
ATCAACG1768550.042.47952311
AACGCAG1769600.042.4569714
TATCAAC1773900.042.33550610
TGGTATC1772250.042.3218847
CGCAGAG1788550.041.9834816
AGCAGTG1792750.041.971642
GCAGTGG1796700.041.830973
AGAGTAC1776950.041.8065819
CAGTGGT1803150.041.6213874
CAGAGTA1803000.041.3394118
GCAGAGT1822800.041.0592517
ACATGGG1151200.040.6248724