##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805209_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	20128486
Sequences flagged as poor quality	0
Sequence length	53
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	34.68605785849964	35.0	35.0	35.0	33.0	37.0
2	35.51502497505277	36.0	35.0	37.0	35.0	37.0
3	37.873725624470715	39.0	37.0	39.0	35.0	39.0
4	37.83901963615147	39.0	38.0	39.0	35.0	39.0
5	37.8645274661989	39.0	38.0	39.0	35.0	39.0
6	37.79062647831536	39.0	38.0	39.0	35.0	39.0
7	37.69156423389221	39.0	37.0	39.0	35.0	39.0
8	38.701122329816556	40.0	39.0	40.0	36.0	40.0
9	38.64719527340506	40.0	39.0	40.0	36.0	40.0
10	38.545714913680044	40.0	38.0	40.0	36.0	40.0
11	38.49403094698727	40.0	38.0	40.0	36.0	40.0
12	38.529529791758804	40.0	38.0	40.0	36.0	40.0
13	38.58034126362013	40.0	38.0	40.0	36.0	40.0
14	38.48478340596506	40.0	38.0	40.0	36.0	40.0
15	38.42015072569293	40.0	38.0	40.0	36.0	40.0
16	38.350049228739806	40.0	38.0	40.0	35.0	40.0
17	38.16003260255143	40.0	38.0	40.0	35.0	40.0
18	38.10591988885801	40.0	38.0	40.0	34.0	40.0
19	38.0373187034534	40.0	38.0	40.0	34.0	40.0
20	37.702497992149034	39.0	37.0	40.0	34.0	40.0
21	37.52154026885082	39.0	37.0	40.0	34.0	40.0
22	37.512926605607596	39.0	37.0	40.0	34.0	40.0
23	37.22623122275565	38.0	37.0	40.0	33.0	40.0
24	37.09123483008111	38.0	36.0	40.0	33.0	40.0
25	36.84936656438045	38.0	36.0	40.0	32.0	40.0
26	36.85823653105355	38.0	36.0	40.0	32.0	40.0
27	36.67061864464123	38.0	36.0	40.0	32.0	40.0
28	36.47477803347952	38.0	36.0	40.0	31.0	40.0
29	36.2123563590426	38.0	35.0	39.0	31.0	40.0
30	35.97746989018449	38.0	35.0	39.0	31.0	40.0
31	35.74574660011687	38.0	34.0	39.0	30.0	40.0
32	35.48969361133272	37.0	34.0	39.0	30.0	40.0
33	35.173856543408185	37.0	34.0	39.0	30.0	40.0
34	34.91965545744474	37.0	33.0	38.0	29.0	40.0
35	34.651807493121936	36.0	33.0	38.0	29.0	40.0
36	34.452123572532976	36.0	33.0	38.0	29.0	39.0
37	34.04273997557491	35.0	32.0	38.0	27.0	39.0
38	33.763510131859896	35.0	32.0	38.0	27.0	39.0
39	33.61246379881726	35.0	31.0	38.0	27.0	39.0
40	33.45348338667896	35.0	31.0	38.0	27.0	39.0
41	33.16988928029659	35.0	31.0	38.0	26.0	39.0
42	32.83909410772375	34.0	31.0	37.0	25.0	38.0
43	32.390039668159844	34.0	30.0	37.0	24.0	38.0
44	32.12044398172819	34.0	30.0	37.0	24.0	38.0
45	32.02346251973447	34.0	30.0	37.0	24.0	38.0
46	31.791539612070178	33.0	29.0	36.0	24.0	38.0
47	31.585019111720573	33.0	29.0	36.0	24.0	38.0
48	31.226079894930994	33.0	29.0	36.0	23.0	38.0
49	30.922170450375653	32.0	28.0	36.0	23.0	38.0
50	30.498905133749254	32.0	28.0	35.0	22.0	38.0
51	30.09641013238651	32.0	27.0	35.0	21.0	37.0
52	29.817119827094796	31.0	27.0	35.0	20.0	37.0
53	31.676303175509574	33.0	30.0	36.0	24.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	13.0
12	58.0
13	241.0
14	814.0
15	1795.0
16	3125.0
17	4260.0
18	5980.0
19	9291.0
20	15780.0
21	25867.0
22	39130.0
23	56587.0
24	78124.0
25	102964.0
26	134892.0
27	172490.0
28	219050.0
29	276442.0
30	351918.0
31	458515.0
32	620859.0
33	902010.0
34	1468877.0
35	2960887.0
36	6146976.0
37	5407050.0
38	661508.0
39	2981.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	12.38831375593773	44.32846067011697	22.85327868176474	20.429946892180563
2	27.47985616007086	41.81855008866538	17.1010626432609	13.600531108002858
3	33.804812741504755	24.83774487559571	21.50593939355399	19.851502989345548
4	21.312531901306436	25.861612244457927	21.58273602892935	31.243119825306287
5	20.135637623217164	34.034164318170774	24.990066316959954	20.840131741652105
6	29.361284301263396	23.91715402738189	23.935371989726402	22.786189681628315
7	23.131431743053106	24.229537184267112	31.446910612154337	21.192120460525445
8	28.571478252263983	25.042231194139493	25.020123222382452	21.36616733121408
9	29.26251880047014	24.152467304297005	25.227406571959758	21.357607323273097
10	21.580579880672595	25.30510243045602	31.828563757850443	21.285753931020942
11	21.19815668202765	32.92000203095255	24.645738382906693	21.236102904113103
12	21.33726302117308	24.68006784017437	32.13018604578606	21.8524830928665
13	21.391141887174225	25.41910504346924	24.102190298863015	29.08756277049352
14	21.600621129676618	33.21562784205429	24.0689736923085	21.11477733596059
15	21.50899973301519	32.60869198011216	23.992847748211165	21.889460538661474
16	21.91283537172145	24.759596921497227	24.083545081333988	29.244022625447343
17	29.58283101868665	24.569980076991385	24.20884511631923	21.638343788002736
18	21.656879707693864	24.565548546472893	24.115037762899803	29.662533982933443
19	21.949350785747125	32.56936463080234	24.008482307114402	21.472802276336132
20	29.73853075685871	24.91914692441349	24.123235100742303	21.219087217985496
21	22.19649803765668	32.1891522293331	24.012670401539392	21.601679331470834
22	29.42650530198844	24.665352376726197	24.37626456356429	21.53187775772107
23	21.711762126570274	24.848987648648784	32.13188513035705	21.30736509442389
24	21.877149627647107	32.03944896799491	24.025766269753223	22.05763513460476
25	21.662543322930496	24.84474987338839	24.02759452449628	29.465112279184837
26	21.819266486311985	29.59102338844561	27.194752749908762	21.394957375333647
27	22.24064442800119	24.180194178538812	31.873112562961765	21.706048830498233
28	27.113350701090983	24.509796712976822	26.762852407279915	21.614000178652283
29	27.140605607396402	24.553168082288952	26.82936014164205	21.476866168672597
30	27.13321806717107	24.21887070890478	26.791746781153837	21.85616444277031
31	25.28063958710059	25.24319017336922	27.119158390750304	22.357011848779884
32	24.074229924694784	26.00549787996971	27.60520090780797	22.315071287527537
33	23.92499366320944	25.374287961846708	27.666238782191567	23.034479592752284
34	23.419804152185115	25.55842997829047	27.85805151962249	23.163714349901927
35	23.210498792606657	25.627794360688625	28.20223041116952	22.95947643553519
36	23.432159775951355	25.369533505898055	28.138827729020456	23.05947898913013
37	23.66365259662351	25.56176853042996	27.946632449156883	22.827946423789648
38	23.671119626185497	25.592893573813747	28.173177058622294	22.562809741378462
39	23.69482235275917	25.324651839189492	28.11064875917642	22.869877048874912
40	23.36346111674768	25.90142646595477	27.79962685718141	22.935485560116145
41	23.528396522222288	26.03460588143589	27.821128722746458	22.61586887359536
42	23.61261547440776	25.71046823889288	27.818813595816394	22.858102690882962
43	23.41112491023915	25.85090602442727	28.042993397516337	22.69497566781724
44	23.535088530751892	25.672348133883492	28.01861997966464	22.773943355699977
45	23.743107156693256	25.172712940257902	27.868653409898787	23.21552649315006
46	23.44350191067525	25.523395053159987	27.68114800089783	23.351955035266936
47	23.364668361048118	25.574531537046553	27.91062874773592	23.15017135416941
48	23.369994146603972	25.211647810967996	27.900359719056862	23.517998323371167
49	23.08399648140451	25.938244933076437	27.656436753365355	23.321321832153696
50	23.55789203420466	25.833910210633825	27.70770737550753	22.90049037965399
51	23.571097200256393	25.375788323076065	27.781607618178533	23.27150685848901
52	23.709026103602625	25.644213876791326	27.41697015861004	23.229789860996004
53	23.974058456259453	25.802959050173968	27.253773582374752	22.969208911191828
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	6535.0
1	4196.5
2	1858.0
3	1511.5
4	1165.0
5	1143.0
6	1121.0
7	1210.5
8	1619.5
9	1939.0
10	2985.5
11	4032.0
12	5915.5
13	7799.0
14	9886.5
15	11974.0
16	17505.0
17	23036.0
18	32477.5
19	41919.0
20	57928.0
21	73937.0
22	285634.5
23	497332.0
24	375364.0
25	257530.0
26	261664.0
27	287598.5
28	313533.0
29	345789.5
30	378046.0
31	409724.0
32	441402.0
33	490363.0
34	539324.0
35	602781.5
36	666239.0
37	734102.0
38	801965.0
39	869616.0
40	937267.0
41	1012500.5
42	1155918.5
43	1224103.0
44	1291280.5
45	1358458.0
46	1407173.0
47	1455888.0
48	1483145.0
49	1510402.0
50	1501414.5
51	1492427.0
52	1457795.0
53	1423163.0
54	1364002.5
55	1304842.0
56	1242559.5
57	1180277.0
58	1042750.0
59	786874.5
60	668526.0
61	576742.5
62	484959.0
63	409371.5
64	333784.0
65	269927.0
66	206070.0
67	160309.0
68	114548.0
69	86612.5
70	58677.0
71	43003.0
72	27329.0
73	20624.5
74	13920.0
75	10189.0
76	4569.0
77	2680.0
78	1930.0
79	1180.0
80	917.0
81	654.0
82	460.0
83	266.0
84	172.5
85	79.0
86	53.0
87	27.0
88	21.0
89	15.0
90	11.5
91	8.0
92	6.0
93	2.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	2.0128486E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.99154963725196
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.53942989685315	31.736782615877228
2	14.681326540852535	13.21071263606943
3	5.362394630985157	7.237873326435064
4	2.5833154965451306	4.649094695659698
5	1.5135730790194228	3.404899915715532
6	1.0130916439273274	2.734833779090493
7	0.7285419445478081	2.2944761742659936
8	0.546965411005216	1.9687057151280878
9	0.426262732865682	1.7260398813813305
>10	2.4063317133481905	19.710478589771586
>50	0.15075688489444805	4.576105387726033
>100	0.04591463776871688	3.348747971304945
>500	0.0014197145999501162	0.42282009631428097
>1k	5.383258272574765E-4	0.46965386192235925
>5k	4.578232005990286E-5	0.1396659273154201
>10k+	9.156464011980373E-5	2.3691094260226255
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	329188	1.63543348466447	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGG	33159	0.16473668213297313	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCAGTG	26053	0.12943348049127987	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.439358727725473E-5	4.968083540908144E-6	0.0	0.0	0.0
2	9.936167081816287E-5	4.968083540908144E-6	0.0	0.0	0.0
3	1.0432975435907101E-4	4.968083540908144E-6	0.0	0.0	4.968083540908144E-6
4	1.4407442268633616E-4	4.968083540908144E-6	0.0	4.968083540908144E-6	9.936167081816288E-6
5	1.5401058976815247E-4	4.968083540908144E-6	0.0	1.4904250622724432E-5	1.4904250622724432E-5
6	1.589786733090606E-4	4.968083540908144E-6	0.0	1.4904250622724432E-5	1.9872334163632575E-5
7	1.6394675684996876E-4	4.968083540908144E-6	0.0	1.4904250622724432E-5	1.9872334163632575E-5
8	1.7885100747269317E-4	4.968083540908144E-6	0.0	1.4904250622724432E-5	2.4840417704540718E-5
9	1.8381909101360133E-4	4.968083540908144E-6	0.0	1.9872334163632575E-5	3.974466832726515E-5
10	1.8381909101360133E-4	4.968083540908144E-6	0.0	2.4840417704540718E-5	4.9680835409081436E-5
11	1.8878717455450946E-4	9.936167081816288E-6	0.0	2.4840417704540718E-5	6.458508603180587E-5
12	2.036914251772339E-4	9.936167081816288E-6	0.0	2.4840417704540718E-5	7.94893366545303E-5
13	2.2356375934086647E-4	9.936167081816288E-6	0.0	3.974466832726515E-5	7.94893366545303E-5
14	2.3846800996359091E-4	9.936167081816288E-6	0.0	3.974466832726515E-5	7.94893366545303E-5
15	2.3846800996359091E-4	1.4904250622724432E-5	0.0	3.974466832726515E-5	7.94893366545303E-5
16	2.583403441272235E-4	1.4904250622724432E-5	0.0	5.464891894998958E-5	7.94893366545303E-5
17	2.831807618317642E-4	1.4904250622724432E-5	0.0	5.464891894998958E-5	9.439358727725473E-5
18	3.0305309599539676E-4	1.4904250622724432E-5	0.0	5.464891894998958E-5	9.439358727725473E-5
19	3.179573466181212E-4	2.9808501245448864E-5	0.0	5.464891894998958E-5	1.0432975435907101E-4
20	3.427977643226619E-4	3.477658478635701E-5	0.0	5.464891894998958E-5	1.2917017206361174E-4
21	3.9247859973174336E-4	3.974466832726515E-5	0.0	5.464891894998958E-5	1.3910633914542803E-4
22	4.3719135159991663E-4	3.974466832726515E-5	0.0	5.464891894998958E-5	1.4407442268633616E-4
23	4.7693601992718183E-4	3.974466832726515E-5	0.0	6.458508603180587E-5	1.4904250622724431E-4
24	5.017764376317225E-4	3.974466832726515E-5	0.0	6.458508603180587E-5	1.7885100747269317E-4
25	5.464891894998959E-4	3.974466832726515E-5	0.0	7.452125311362216E-5	1.7885100747269317E-4
26	6.061061919907935E-4	3.974466832726515E-5	0.0	7.94893366545303E-5	1.7885100747269317E-4
27	6.90563612186232E-4	4.471275186817329E-5	0.0	8.445742019543844E-5	1.8878717455450946E-4
28	7.452125311362216E-4	4.471275186817329E-5	0.0	8.445742019543844E-5	2.1362759225905019E-4
29	8.097976171680274E-4	4.471275186817329E-5	0.0	8.445742019543844E-5	2.1859567579995832E-4
30	9.141273715270985E-4	4.471275186817329E-5	0.0	8.445742019543844E-5	2.2853184288177463E-4
31	0.0010134890423452614	4.471275186817329E-5	0.0	8.942550373634659E-5	2.2853184288177463E-4
32	0.0011128507131634242	4.471275186817329E-5	0.0	9.439358727725473E-5	2.3349992642268276E-4
33	0.001246988968767944	4.471275186817329E-5	0.0	9.439358727725473E-5	2.3846800996359091E-4
34	0.0014009995585360965	4.471275186817329E-5	0.0	9.936167081816287E-5	2.3846800996359091E-4
35	0.001515265479976984	4.9680835409081436E-5	0.0	9.936167081816287E-5	2.4343609350449904E-4
36	0.0016444356520405956	4.9680835409081436E-5	0.0	1.1923400498179546E-4	2.633084276681316E-4
37	0.0017487654063996665	4.9680835409081436E-5	0.0	1.1923400498179546E-4	2.633084276681316E-4
38	0.001932584497413268	4.9680835409081436E-5	0.0	1.1923400498179546E-4	2.6827651120903974E-4
39	0.0021014993378041446	4.9680835409081436E-5	0.0	1.2420208852270359E-4	2.7324459474994793E-4
40	0.002349903514849552	4.9680835409081436E-5	0.0	1.2917017206361174E-4	2.7324459474994793E-4
41	0.002523786438781337	4.9680835409081436E-5	0.0	1.3413825560451987E-4	3.0305309599539676E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	169310	0.0	44.41696	1
TCAACGC	175405	0.0	42.813263	12
CAACGCA	175720	0.0	42.752563	13
GTGGTAT	175965	0.0	42.639618	6
GTATCAA	176595	0.0	42.510124	9
GGTATCA	176710	0.0	42.509056	8
ACGCAGA	176690	0.0	42.49658	15
AGTGGTA	176740	0.0	42.483227	5
ATCAACG	176855	0.0	42.479523	11
AACGCAG	176960	0.0	42.45697	14
TATCAAC	177390	0.0	42.335506	10
TGGTATC	177225	0.0	42.321884	7
CGCAGAG	178855	0.0	41.98348	16
AGCAGTG	179275	0.0	41.97164	2
GCAGTGG	179670	0.0	41.83097	3
AGAGTAC	177695	0.0	41.80658	19
CAGTGGT	180315	0.0	41.621387	4
CAGAGTA	180300	0.0	41.33941	18
GCAGAGT	182280	0.0	41.05925	17
ACATGGG	115120	0.0	40.62487	24
>>END_MODULE