FastQCFastQC Report
Thu 26 May 2016
SRR805208_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805208_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17031937
Sequences flagged as poor quality0
Sequence length53
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT6089663.5754359589282183No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCC570550.3349883222325212No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGG532510.3126538103094205No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCAGTG448470.26331121351611386No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTA284470.16702151963103198No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTG271390.15934182941141692No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTC192300.11290553740305638No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT3610000.045.901161
GTGGTAT3661400.045.111096
TCAACGC3658500.045.0684812
TGGTATC3665300.045.0592467
AGTGGTA3680400.045.058275
GTATCAA3678300.045.0539639
CAACGCA3677500.045.0477913
AGCAGTG3696200.045.0430452
GGTATCA3679800.045.0349588
TATCAAC3688800.044.96776610
ATCAACG3678600.044.96340611
ACGCAGA3686350.044.9562215
AACGCAG3686950.044.9482614
CGCAGAG3701850.044.77623416
AGAGTAC3665750.044.7639519
CAGTGGT3709500.044.7009964
GCAGTGG3720600.044.6674353
CAGAGTA3680450.044.61134318
GCAGAGT3704400.044.38952317
ACATGGG2619400.044.30854424
Produced by FastQC (version 0.11.5)