##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805208_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	17031937
Sequences flagged as poor quality	0
Sequence length	53
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	34.67129998191045	35.0	35.0	35.0	33.0	37.0
2	35.57008330878631	36.0	35.0	37.0	35.0	37.0
3	37.91361540381461	39.0	38.0	39.0	35.0	39.0
4	37.92237077908403	39.0	38.0	39.0	35.0	39.0
5	37.90253204905584	39.0	38.0	39.0	35.0	39.0
6	37.91322431500304	39.0	38.0	39.0	35.0	39.0
7	37.7367945877207	39.0	38.0	39.0	35.0	39.0
8	38.73257938894443	40.0	39.0	40.0	36.0	40.0
9	38.670626658611994	40.0	39.0	40.0	36.0	40.0
10	38.25958556563472	40.0	38.0	40.0	35.0	40.0
11	38.51185728317337	40.0	38.0	40.0	36.0	40.0
12	38.45511212259651	40.0	38.0	40.0	36.0	40.0
13	38.69790541146318	40.0	39.0	40.0	36.0	40.0
14	38.7219137200895	40.0	39.0	40.0	36.0	40.0
15	38.678837820971275	40.0	39.0	40.0	36.0	40.0
16	38.60323150561207	40.0	39.0	40.0	36.0	40.0
17	38.28023365751059	40.0	38.0	40.0	35.0	40.0
18	38.368234687575466	40.0	38.0	40.0	35.0	40.0
19	38.38683768029438	40.0	38.0	40.0	35.0	40.0
20	38.368709384023674	40.0	38.0	40.0	35.0	40.0
21	38.286535465696005	40.0	38.0	40.0	35.0	40.0
22	38.251224332264734	40.0	38.0	40.0	35.0	40.0
23	37.43000939940067	39.0	37.0	40.0	34.0	40.0
24	37.592784837097504	39.0	37.0	40.0	34.0	40.0
25	37.69254360205771	39.0	38.0	40.0	34.0	40.0
26	37.49446613147994	39.0	37.0	40.0	34.0	40.0
27	37.538892493554904	39.0	37.0	40.0	33.0	40.0
28	37.45641743508093	39.0	37.0	40.0	33.0	40.0
29	37.24436345672251	39.0	37.0	40.0	33.0	40.0
30	37.1744637735567	39.0	37.0	40.0	33.0	40.0
31	36.93020523737259	38.0	36.0	40.0	32.0	40.0
32	36.64726372578762	38.0	36.0	40.0	31.0	40.0
33	36.47072485061447	38.0	35.0	40.0	31.0	40.0
34	36.3607255005699	38.0	35.0	40.0	31.0	40.0
35	36.12432385112744	38.0	35.0	40.0	31.0	40.0
36	35.96320770796651	38.0	35.0	39.0	30.0	40.0
37	35.64580693317501	38.0	35.0	39.0	30.0	40.0
38	35.60734184256318	37.0	35.0	39.0	30.0	40.0
39	35.4850824072447	37.0	34.0	39.0	30.0	40.0
40	35.392332122881854	37.0	34.0	38.0	30.0	40.0
41	35.143897314791616	37.0	34.0	38.0	30.0	40.0
42	34.967851337167346	37.0	34.0	38.0	29.0	40.0
43	34.6444181304804	36.0	33.0	38.0	28.0	40.0
44	34.49155201783567	36.0	33.0	38.0	28.0	39.0
45	34.39912635891032	36.0	33.0	38.0	28.0	39.0
46	34.308863930156626	36.0	33.0	38.0	28.0	39.0
47	34.06775312755091	36.0	33.0	38.0	28.0	39.0
48	33.91080075037854	35.0	33.0	38.0	27.0	39.0
49	33.53520383500714	35.0	32.0	38.0	26.0	39.0
50	33.28829739095442	35.0	32.0	38.0	26.0	39.0
51	32.89847525856865	34.0	31.0	37.0	26.0	38.0
52	32.31302763743196	34.0	31.0	37.0	24.0	38.0
53	34.19825836603318	36.0	33.0	39.0	26.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	3.0
12	21.0
13	72.0
14	281.0
15	783.0
16	1675.0
17	2892.0
18	4679.0
19	7542.0
20	12084.0
21	19090.0
22	28602.0
23	39166.0
24	53930.0
25	72380.0
26	93554.0
27	120993.0
28	150959.0
29	186126.0
30	230135.0
31	289514.0
32	374042.0
33	504197.0
34	721953.0
35	1134484.0
36	2080651.0
37	4426950.0
38	6121986.0
39	353192.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	13.899012191038517	49.492374237880284	20.852272997487013	15.756340573594182
2	23.71363867773818	49.26904086129487	13.561869093339176	13.455451367627768
3	44.023871154525764	21.484913900280397	17.277171703958274	17.21404324123557
4	20.06648451083397	21.163958039534787	17.536308406965105	41.23324904266614
5	18.344331593053685	42.52191632695682	20.599312926063547	18.534439153925945
6	40.11443325559506	20.352294633311523	19.605791167499035	19.92748094359438
7	20.573255995486598	20.289700460963424	40.55946190970528	18.5775816338447
8	39.54898377090051	21.301065169510665	20.57002676794777	18.57992429164105
9	39.92154855903941	20.680055357179867	20.66234157629869	18.736054507482034
10	19.047821748048975	21.272325044415087	41.027388722727196	18.652464484808746
11	18.66703123667026	41.916676887661104	20.65109799314077	18.765193882527864
12	18.976150510655366	20.926639172044847	41.107872815640405	18.98933750165938
13	18.770906679610196	21.376940274027554	20.36438955827514	39.48776348808711
14	18.92867499451178	42.15248682519199	20.300673963272644	18.618164217023583
15	18.941315952495597	41.699079793449215	20.26784739750975	19.09175685654544
16	19.050440358016825	20.83987863506071	20.39464448465257	39.7150365222699
17	39.715377058992175	20.86052807734082	20.354754717563832	19.06934014610317
18	19.083231695842933	20.67822937578973	20.39502024931163	39.843518679055705
19	19.116692364467998	41.753348430069934	20.288338314074316	18.841620891387752
20	40.06642344907687	20.95331846283837	20.386618386387877	18.593639701696876
21	19.347353151905153	41.364426136616174	20.411823975159137	18.87639673631954
22	39.840659344853144	20.840823918031166	20.50069231702771	18.81782442008798
23	19.34956664059995	20.86042239353046	41.036912008305336	18.75309895756425
24	19.140559291641342	41.22275111750355	20.34030539215827	19.296384198696835
25	19.033536819681753	20.95911345843987	20.367213664540916	39.64013605733746
26	19.475277533025164	35.71378874874889	26.022002077626283	18.788931640599657
27	19.60014882629028	20.47769434562845	40.9509734565129	18.971183371568365
28	34.572339012292026	20.650522603506577	25.888382513392337	18.888755870809057
29	34.69939443763795	20.696694685989034	25.855996296839283	18.74791457953373
30	34.38180871617832	20.4907991381133	25.937813180027614	19.189578965680766
31	29.003906014917742	22.892716195462675	27.108948324550518	20.994429465069064
32	25.09668160468184	25.251073908974654	28.243587326561858	21.40865715978165
33	24.330732317762802	24.023656264111356	28.96909494204916	22.676516476076678
34	23.486377386200992	23.76147821589523	29.699557953977873	23.0525864439259
35	23.028103027858783	23.98209317002523	29.985608800690137	23.00419500142585
36	22.931478668574222	24.047987025785734	30.08490461184773	22.935629693792315
37	23.994593216261897	23.81509513568539	29.722127318812884	22.468184329239826
38	24.142133686849593	23.89877909952344	29.75349779652191	22.205589417105053
39	24.136009897171416	23.699265679528995	29.64049831795409	22.524226105345505
40	23.48032405239639	24.32961089510841	29.159636980808468	23.030428071686735
41	23.64778592123726	25.015768905204382	29.043590285708547	22.29285488784981
42	23.913774457949206	24.67799757596567	29.16095215711519	22.24727580896994
43	23.908173216000037	24.33685023611818	29.735337795108098	22.019638752773687
44	23.752230882488586	24.08859309425581	29.46552702725474	22.693648996000864
45	23.923426912628905	23.439782568477092	29.309437910673342	23.32735260822066
46	23.70823706076414	23.744474865072597	28.980315039915894	23.56697303424737
47	23.549135955587435	23.93819328946555	29.163817362640547	23.348853392306467
48	23.575621492728633	23.68857987203687	29.290602707137776	23.44519592809673
49	22.88936954146789	24.80487686162766	28.903770604600055	23.401982992304397
50	23.837224151310565	24.477985093533402	28.87294615991123	22.811844595244803
51	23.65352807493358	23.970520792790627	29.007604948280395	23.3683461839954
52	24.144505701259934	24.106254033231806	28.51602257570586	23.233217689802398
53	24.704148447707386	24.276152501033792	27.90933291967907	23.11036613157975
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3836.0
1	2368.5
2	901.0
3	738.0
4	575.0
5	571.0
6	567.0
7	635.0
8	890.0
9	1077.0
10	1671.5
11	2266.0
12	3365.5
13	4465.0
14	5716.0
15	6967.0
16	10221.5
17	13476.0
18	19199.5
19	24923.0
20	36935.0
21	48947.0
22	402535.0
23	756123.0
24	496561.5
25	216348.0
26	195696.0
27	203487.5
28	211279.0
29	227605.5
30	243932.0
31	262470.0
32	281008.0
33	314004.0
34	347000.0
35	390550.5
36	434101.0
37	485788.5
38	537476.0
39	593047.0
40	648618.0
41	713399.0
42	842061.5
43	905943.0
44	982005.5
45	1058068.0
46	1119712.0
47	1181356.0
48	1254249.5
49	1327143.0
50	1325091.0
51	1323039.0
52	1308555.0
53	1294071.0
54	1269628.5
55	1245186.0
56	1215743.5
57	1186301.0
58	1031453.0
59	757364.0
60	638123.0
61	549944.5
62	461766.0
63	390535.5
64	319305.0
65	258535.0
66	197765.0
67	156191.5
68	114618.0
69	88568.0
70	62518.0
71	46130.0
72	29742.0
73	22727.5
74	15713.0
75	12045.0
76	6059.5
77	3742.0
78	2702.5
79	1663.0
80	1329.5
81	996.0
82	713.0
83	430.0
84	310.0
85	190.0
86	130.5
87	71.0
88	50.5
89	30.0
90	29.5
91	29.0
92	22.0
93	11.5
94	8.0
95	5.5
96	3.0
97	2.5
98	2.0
99	2.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	1.7031937E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.16005264286793
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.5769273352937	32.933210370699065
2	12.695919964247132	11.213049879415827
3	4.433067795623066	5.872935216723513
4	2.053831786681957	3.6278927927788294
5	1.2161922879683857	2.685355773026696
6	0.7873571061943516	2.0861838754967232
7	0.5254990004848821	1.624424446763081
8	0.4399620999320228	1.554299959509188
9	0.3517297549309914	1.3979164044434054
>10	2.7576665480035443	22.84029134135731
>50	0.1168263876907633	3.4647820494417405
>100	0.04093272861780396	3.268839662281881
>500	0.00234052549607581	0.7037725341501047
>1k	0.0014667297305980902	1.1983172566039757
>5k	1.7330182681624053E-4	0.5022557840326879
>10k+	1.0664727804076336E-4	5.026472653276016
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	608966	3.5754359589282183	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCC	57055	0.3349883222325212	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGG	53251	0.3126538103094205	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCAGTG	44847	0.26331121351611386	No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTA	28447	0.16702151963103198	No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTG	27139	0.15934182941141692	No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTC	19230	0.11290553740305638	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.9375365232973796E-4	0.0	0.0	5.871322797870847E-6	5.871322797870847E-6
2	1.9962497512760879E-4	0.0	0.0	5.871322797870847E-6	1.761396839361254E-5
3	2.0549629792547964E-4	0.0	0.0	5.871322797870847E-6	1.761396839361254E-5
4	3.229227538828966E-4	0.0	0.0	1.1742645595741694E-5	1.761396839361254E-5
5	3.229227538828966E-4	0.0	0.0	1.1742645595741694E-5	2.3485291191483387E-5
6	3.3466539947863826E-4	0.0	0.0	1.761396839361254E-5	2.3485291191483387E-5
7	3.4640804507438E-4	0.0	0.0	1.761396839361254E-5	2.3485291191483387E-5
8	3.4640804507438E-4	0.0	0.0	2.3485291191483387E-5	2.9356613989354234E-5
9	3.581506906701217E-4	0.0	0.0	2.9356613989354234E-5	3.522793678722508E-5
10	3.640220134679925E-4	5.871322797870847E-6	0.0	3.522793678722508E-5	3.522793678722508E-5
11	3.8163598186160506E-4	5.871322797870847E-6	0.0	4.1099259585095926E-5	5.2841905180837624E-5
12	3.9337862745734677E-4	5.871322797870847E-6	0.0	4.1099259585095926E-5	5.2841905180837624E-5
13	4.051212730530884E-4	5.871322797870847E-6	0.0	4.6970582382966775E-5	5.871322797870847E-5
14	4.3447788704244265E-4	5.871322797870847E-6	0.0	5.871322797870847E-5	7.632719637232101E-5
15	4.6970582382966774E-4	5.871322797870847E-6	0.0	6.458455077657932E-5	8.219851917019185E-5
16	4.8144846942540945E-4	5.871322797870847E-6	0.0	6.458455077657932E-5	9.394116476593355E-5
17	4.873197922232803E-4	5.871322797870847E-6	0.0	6.458455077657932E-5	1.0568381036167525E-4
18	5.166764062126346E-4	5.871322797870847E-6	0.0	6.458455077657932E-5	1.1742645595741693E-4
19	5.460330202019888E-4	5.871322797870847E-6	0.0	6.458455077657932E-5	1.232977787552878E-4
20	5.695183113934722E-4	5.871322797870847E-6	0.0	6.458455077657932E-5	1.3504042435102948E-4
21	5.988749253828264E-4	5.871322797870847E-6	0.0	7.045587357445017E-5	1.3504042435102948E-4
22	6.164888937764389E-4	5.871322797870847E-6	0.0	7.045587357445017E-5	1.467830699467712E-4
23	6.810734445530182E-4	5.871322797870847E-6	0.0	7.045587357445017E-5	1.5852571554251287E-4
24	7.574006409253393E-4	5.871322797870847E-6	0.0	7.045587357445017E-5	1.643970383403837E-4
25	7.926285777125644E-4	5.871322797870847E-6	0.0	7.632719637232101E-5	1.7026836113825456E-4
26	8.865697424784979E-4	5.871322797870847E-6	0.0	7.632719637232101E-5	1.7613968393612541E-4
27	9.863822300423023E-4	5.871322797870847E-6	0.0	8.219851917019185E-5	1.7613968393612541E-4
28	0.0010627094264146232	5.871322797870847E-6	0.0	8.219851917019185E-5	1.9375365232973796E-4
29	0.0011625219139784278	1.1742645595741694E-5	0.0	8.806984196806271E-5	1.9375365232973796E-4
30	0.0012564630787443613	1.1742645595741694E-5	0.0	9.981248756380439E-5	2.0549629792547964E-4
31	0.001379760857499649	1.1742645595741694E-5	0.0	9.981248756380439E-5	2.0549629792547964E-4
32	0.001497187313457066	1.761396839361254E-5	0.0	1.0568381036167525E-4	2.1723894352122133E-4
33	0.0016968122885846748	1.761396839361254E-5	0.0	1.1155513315954609E-4	2.2898158911696304E-4
34	0.0018494666813293169	1.761396839361254E-5	0.0	1.1155513315954609E-4	2.4072423471270472E-4
35	0.0020138637196697004	1.761396839361254E-5	0.0	1.1155513315954609E-4	2.583382031063173E-4
36	0.0021430328212228593	1.761396839361254E-5	0.0	1.1742645595741693E-4	2.7008084870205895E-4
37	0.0023191725051589845	1.761396839361254E-5	0.0	1.1742645595741693E-4	2.7008084870205895E-4
38	0.002501183511892981	1.761396839361254E-5	0.0	1.1742645595741693E-4	2.8182349429780066E-4
39	0.0027771356833929104	2.3485291191483387E-5	0.0	1.1742645595741693E-4	2.8182349429780066E-4
40	0.0030413452092970987	2.3485291191483387E-5	0.0	1.232977787552878E-4	2.8182349429780066E-4
41	0.0033877532543714786	2.3485291191483387E-5	0.0	1.2916910155315865E-4	2.8182349429780066E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	361000	0.0	45.90116	1
GTGGTAT	366140	0.0	45.11109	6
TCAACGC	365850	0.0	45.06848	12
TGGTATC	366530	0.0	45.059246	7
AGTGGTA	368040	0.0	45.05827	5
GTATCAA	367830	0.0	45.053963	9
CAACGCA	367750	0.0	45.04779	13
AGCAGTG	369620	0.0	45.043045	2
GGTATCA	367980	0.0	45.034958	8
TATCAAC	368880	0.0	44.967766	10
ATCAACG	367860	0.0	44.963406	11
ACGCAGA	368635	0.0	44.95622	15
AACGCAG	368695	0.0	44.94826	14
CGCAGAG	370185	0.0	44.776234	16
AGAGTAC	366575	0.0	44.76395	19
CAGTGGT	370950	0.0	44.700996	4
GCAGTGG	372060	0.0	44.667435	3
CAGAGTA	368045	0.0	44.611343	18
GCAGAGT	370440	0.0	44.389523	17
ACATGGG	261940	0.0	44.308544	24
>>END_MODULE