FastQCFastQC Report
Thu 26 May 2016
SRR805207_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805207_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25160196
Sequences flagged as poor quality0
Sequence length53
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT10261334.078398276388626No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCC1013450.40279892891136465No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTA524740.20855958355809312No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGG480600.19101600003433994No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCAGTG471140.18725609291755915No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTG464380.18456930939647687No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTC360410.14324610189841128No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT6119850.046.1091271
AGCAGTG6240500.045.2045022
AGTGGTA6247750.045.123465
GTGGTAT6260750.045.111976
TCAACGC6295250.045.04429612
TGGTATC6247050.045.0397347
GTATCAA6269200.045.0222979
CAACGCA6306350.045.01717813
GGTATCA6271200.045.0053148
ACGCAGA6287800.044.96909315
TATCAAC6281750.044.96788810
AACGCAG6338900.044.9665614
ATCAACG6312600.044.94096411
CGCAGAG6306600.044.8264716
GCAGTGG6290950.044.8106043
CAGTGGT6293000.044.7930224
AGAGTAC6236550.044.77304519
CAGAGTA6289200.044.62167718
GAGTACT1866300.044.54964420
GCAGAGT6334550.044.49550217
Produced by FastQC (version 0.11.5)