##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805207_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	25160196
Sequences flagged as poor quality	0
Sequence length	53
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	34.70622017411947	35.0	35.0	35.0	33.0	37.0
2	35.63877125599498	36.0	35.0	37.0	35.0	37.0
3	37.76385108446691	39.0	39.0	39.0	35.0	39.0
4	37.92915881895355	39.0	39.0	39.0	35.0	39.0
5	37.85419024557678	39.0	39.0	39.0	35.0	39.0
6	37.946080229263714	39.0	39.0	39.0	35.0	39.0
7	37.8089960030518	39.0	38.0	39.0	35.0	39.0
8	38.78941360393218	40.0	39.0	40.0	36.0	40.0
9	38.798724660173555	40.0	39.0	40.0	36.0	40.0
10	38.44709115143618	40.0	38.0	40.0	36.0	40.0
11	38.65861311255286	40.0	39.0	40.0	36.0	40.0
12	38.677934822129366	40.0	39.0	40.0	36.0	40.0
13	38.6610543097518	40.0	39.0	40.0	36.0	40.0
14	38.785378937429584	40.0	39.0	40.0	36.0	40.0
15	38.70763204706354	40.0	39.0	40.0	36.0	40.0
16	38.712106336532514	40.0	39.0	40.0	36.0	40.0
17	38.67821319833916	40.0	39.0	40.0	36.0	40.0
18	38.568562701180866	40.0	39.0	40.0	36.0	40.0
19	38.52102610806371	40.0	39.0	40.0	36.0	40.0
20	38.44252024109828	40.0	38.0	40.0	36.0	40.0
21	38.035818997594454	40.0	38.0	40.0	35.0	40.0
22	38.226445334527604	40.0	38.0	40.0	35.0	40.0
23	37.90626440270974	39.0	38.0	40.0	34.0	40.0
24	37.9524808550776	40.0	38.0	40.0	34.0	40.0
25	37.84109185794896	40.0	38.0	40.0	34.0	40.0
26	37.920642907551276	40.0	38.0	40.0	34.0	40.0
27	37.57773520524244	40.0	38.0	40.0	34.0	40.0
28	37.6267497677681	40.0	38.0	40.0	34.0	40.0
29	37.4597933577306	39.0	37.0	40.0	33.0	40.0
30	37.37652449130365	39.0	37.0	40.0	33.0	40.0
31	37.110023983914914	39.0	37.0	40.0	33.0	40.0
32	36.75968716618901	38.0	36.0	40.0	31.0	40.0
33	36.623139819737496	38.0	36.0	40.0	31.0	40.0
34	36.540299686059676	38.0	36.0	40.0	31.0	40.0
35	36.31059869326932	38.0	35.0	40.0	31.0	40.0
36	36.157359147758626	38.0	35.0	39.0	31.0	40.0
37	35.641491982017946	38.0	35.0	39.0	30.0	40.0
38	35.63901676282649	38.0	35.0	39.0	30.0	40.0
39	35.470213507080786	37.0	34.0	39.0	30.0	40.0
40	35.30125429070584	37.0	34.0	38.0	30.0	40.0
41	35.131235702615356	37.0	34.0	38.0	30.0	40.0
42	34.86077214978771	36.0	34.0	38.0	29.0	40.0
43	34.665894017677765	36.0	33.0	38.0	29.0	40.0
44	34.65349113337591	36.0	33.0	38.0	29.0	40.0
45	34.37893079211307	36.0	33.0	38.0	28.0	39.0
46	33.3893159655831	35.0	32.0	38.0	24.0	39.0
47	33.26503012933603	35.0	32.0	38.0	24.0	39.0
48	33.44350373900108	35.0	32.0	38.0	26.0	39.0
49	33.40678653695703	35.0	32.0	38.0	26.0	39.0
50	33.21920620173229	35.0	32.0	38.0	26.0	39.0
51	32.60632464866331	34.0	31.0	37.0	24.0	38.0
52	32.108066805202945	34.0	31.0	37.0	23.0	38.0
53	34.05704474639228	36.0	33.0	39.0	26.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	9.0
12	57.0
13	196.0
14	621.0
15	1539.0
16	3134.0
17	5217.0
18	7474.0
19	11848.0
20	18965.0
21	29943.0
22	43577.0
23	61148.0
24	82703.0
25	109784.0
26	142415.0
27	179637.0
28	223788.0
29	273169.0
30	333769.0
31	412849.0
32	526887.0
33	702065.0
34	1009999.0
35	1606037.0
36	3014451.0
37	6516476.0
38	9331033.0
39	511404.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	13.084886143176307	51.8910067314261	19.470941323350583	15.553165802047012
2	22.344372833979513	51.32719156877792	13.154349036072693	13.174086561169872
3	45.82917796029888	20.56622293403438	16.84384334684833	16.760755758818412
4	19.052681465597484	20.30146347031637	17.077859806815496	43.56799525727065
5	17.67722715673598	44.71648392564192	19.90573920807294	17.70054970954916
6	42.42542069227124	19.552729239470153	19.047896129267038	18.97395393899157
7	19.741670533886143	19.542065570554378	43.01638985642242	17.699874039137057
8	41.96770565698296	20.521545221666795	19.84427704776227	17.66647207358798
9	42.152227271997404	19.953787323437385	20.051215022331302	17.842770382233905
10	18.298382890180985	20.54686298946161	43.45222906848579	17.702525051871614
11	17.759547660121566	44.36418142370592	19.969395309957044	17.90687560621547
12	17.88166117624839	20.20690935793982	43.687739952423264	18.22368951338853
13	17.99023346241023	20.70679815053905	19.587605756330355	41.715362630720364
14	17.970420421208168	44.708034070958746	19.54220865370047	17.779336854132616
15	18.04512572159613	44.171134437903426	19.557840487411145	18.2258993530893
16	18.233260186049424	20.10827340136778	19.585034234232516	42.07343217835028
17	42.096083035283186	20.04320236615009	19.739504414035565	18.121210184531154
18	18.16264070438879	20.033436941429233	19.66736666121361	42.13655569296837
19	18.30861333512664	44.22964749559185	19.545738037970768	17.91600113131074
20	42.36958249450839	20.305724168444474	19.628936912892094	17.695756424155043
21	18.58001026701064	43.71838359287821	19.65612271064979	18.04548342946136
22	42.08517691992542	20.118595260545664	19.877722733161537	17.91850508636737
23	18.147104259442177	20.202863284530853	43.70475492321284	17.94527753281413
24	18.18192115832484	43.735720500746496	19.652271389300783	18.43008695162788
25	18.228594085674054	20.249933665063658	19.69921458481484	41.82225766444745
26	18.357702777832095	36.6221073953478	27.075464753931172	17.94472507288894
27	19.128928884337785	19.807158894946607	43.106897895390006	17.957014325325606
28	35.30181561383703	19.89437999608588	26.849858403328813	17.953945986748273
29	35.26689935165847	19.95256714216376	26.873546613070896	17.906986893106875
30	34.997346602546344	19.78409468670276	26.92362173967166	18.294936971079238
31	28.30461654591244	22.45785764149055	28.461888770659815	20.77563704193719
32	24.46583484484779	25.406590632282832	29.60036161880456	20.527212904064818
33	23.57608024993128	24.065404736910633	30.36495820620793	21.993556806950153
34	22.911419291010294	23.549295879889012	31.159844700732858	22.37944012836784
35	22.406411301406397	24.049486736907774	31.136128669267922	22.407973292417914
36	22.14108347963585	24.16334117587955	31.359827244589034	22.335748099895568
37	23.22631747383844	23.609239769038364	31.09041360409116	22.074029153032036
38	23.46413756077258	23.803053839485194	31.098418311208704	21.634390288533524
39	23.567169349555147	23.586942645438853	30.972727716429553	21.87316028857645
40	22.735578053525497	24.080853742156858	30.53829151410426	22.645276690213382
41	22.84850245204767	24.959642603738065	30.3991670017197	21.792687942494567
42	23.205069626643606	24.579236187190276	30.5862283425773	21.629465843588818
43	23.23591199369035	24.032467791586363	31.177483673020674	21.55413654170262
44	22.819540038559317	23.941995523405303	30.914214658741134	22.32424977929425
45	22.97739651948657	23.346876948017417	30.759478185305074	22.916248347190937
46	23.114978913518797	23.737493936851685	30.163950233138088	22.98357691649143
47	23.07918030527266	23.865601046987077	30.22616755449759	22.829051093242676
48	23.091743800406007	23.660845090395956	30.638485487155982	22.608925622042054
49	22.345072351582633	24.68243490630995	30.27383014027395	22.698662601833465
50	23.092045864825536	24.34047413621102	30.294696432412533	22.272783566550913
51	23.13579751127535	23.967380858241327	30.033017230867358	22.863804399615965
52	23.281631033398945	24.096398136167142	29.644506743906128	22.977464086527785
53	23.670960273918375	24.457297550464233	28.977898264385537	22.89384391123185
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	6076.0
1	4028.0
2	1980.0
3	1561.0
4	1142.0
5	1065.5
6	989.0
7	1088.5
8	1470.0
9	1752.0
10	2670.0
11	3588.0
12	5297.5
13	7007.0
14	9087.0
15	11167.0
16	16515.5
17	21864.0
18	31094.0
19	40324.0
20	61503.5
21	82683.0
22	698122.0
23	1313561.0
24	903676.5
25	425569.5
26	357347.0
27	355717.0
28	354087.0
29	371253.0
30	388419.0
31	411318.0
32	434217.0
33	479486.0
34	524755.0
35	587058.5
36	649362.0
37	724758.5
38	800155.0
39	881555.5
40	962956.0
41	1056386.0
42	1246574.0
43	1343332.0
44	1453290.5
45	1563249.0
46	1656337.0
47	1749425.0
48	1857818.5
49	1966212.0
50	1941364.0
51	1916516.0
52	1888600.0
53	1860684.0
54	1803860.0
55	1747036.0
56	1673742.5
57	1600449.0
58	1405967.5
59	1048470.5
60	885455.0
61	764629.5
62	643804.0
63	549264.5
64	454725.0
65	367764.0
66	280803.0
67	221904.5
68	163006.0
69	125407.0
70	87808.0
71	64150.5
72	40493.0
73	29972.0
74	19451.0
75	14373.5
76	6865.0
77	4434.0
78	3252.5
79	2071.0
80	1684.5
81	1298.0
82	950.5
83	603.0
84	403.0
85	203.0
86	128.5
87	54.0
88	48.0
89	42.0
90	28.0
91	14.0
92	12.0
93	7.0
94	4.0
95	4.0
96	4.0
97	3.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	2.5160196E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.01975555786717
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.94207480860418	26.591805874331904
2	15.228270088520137	11.579502126694317
3	5.4534365261639035	6.220149710252877
4	2.525233454544146	3.8403503467326767
5	1.3721286130612684	2.608399723127237
6	0.8899164802412503	2.0300644227413915
7	0.6424033565503535	1.7096813009918481
8	0.45974785617385855	1.3983600887986862
9	0.356437341574581	1.219649453852542
>10	2.841033022066053	23.640609104249737
>50	0.21899737348433976	5.541105649228439
>100	0.06359835006814278	4.253443685531469
>500	0.004175726181758707	1.0833103964834379
>1k	0.0021899990754274913	1.5481722200815733
>5k	1.7850184533062946E-4	0.47367898778410567
>10k+	1.7850184532978086E-4	6.261716909117672
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1026133	4.078398276388626	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCC	101345	0.40279892891136465	No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTA	52474	0.20855958355809312	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGG	48060	0.19101600003433994	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCAGTG	47114	0.18725609291755915	No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTG	46438	0.18456930939647687	No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTC	36041	0.14324610189841128	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.141423222617185E-5	0.0	0.0	3.974531835920515E-6	0.0
2	1.073123595698539E-4	0.0	0.0	3.974531835920515E-6	0.0
3	1.1923595507761546E-4	0.0	0.0	3.974531835920515E-6	1.1923595507761546E-5
4	1.6295580527274112E-4	0.0	0.0	3.974531835920515E-6	1.1923595507761546E-5
5	1.788539326164232E-4	0.0	0.0	3.974531835920515E-6	1.1923595507761546E-5
6	1.868029962882642E-4	0.0	0.0	7.94906367184103E-6	1.1923595507761546E-5
7	1.9872659179602577E-4	0.0	0.0	1.1923595507761546E-5	1.1923595507761546E-5
8	2.106501873037873E-4	0.0	0.0	1.589812734368206E-5	1.1923595507761546E-5
9	2.106501873037873E-4	0.0	0.0	1.589812734368206E-5	1.1923595507761546E-5
10	2.2654831464746935E-4	0.0	0.0	2.7821722851443605E-5	1.1923595507761546E-5
11	2.3449737831931038E-4	0.0	0.0	2.7821722851443605E-5	2.7821722851443605E-5
12	2.4642097382707195E-4	0.0	0.0	2.7821722851443605E-5	2.7821722851443605E-5
13	2.662936330066745E-4	0.0	0.0	2.7821722851443605E-5	2.7821722851443605E-5
14	2.9808988769403864E-4	0.0	0.0	2.7821722851443605E-5	2.7821722851443605E-5
15	3.179625468736412E-4	0.0	0.0	3.179625468736412E-5	2.7821722851443605E-5
16	3.418097378891643E-4	0.0	0.0	3.179625468736412E-5	3.974531835920515E-5
17	3.736059925765284E-4	0.0	0.0	3.179625468736412E-5	3.974531835920515E-5
18	3.8950411992021047E-4	0.0	0.0	3.179625468736412E-5	4.3719850195125665E-5
19	4.213003746075746E-4	0.0	0.0	3.179625468736412E-5	5.1668913866966693E-5
20	4.491220974590182E-4	0.0	0.0	3.179625468736412E-5	5.564344570288721E-5
21	4.968164794900644E-4	0.0	0.0	3.179625468736412E-5	6.359250937472824E-5
22	5.4053632968519E-4	0.0	0.0	3.179625468736412E-5	6.359250937472824E-5
23	5.922052435521568E-4	3.974531835920515E-6	0.0	3.179625468736412E-5	6.359250937472824E-5
24	6.39899625583203E-4	3.974531835920515E-6	0.0	3.577078652328464E-5	6.756704121064876E-5
25	6.875940076142491E-4	3.974531835920515E-6	0.0	3.974531835920515E-5	7.154157304656927E-5
26	7.472119851530568E-4	3.974531835920515E-6	0.0	3.974531835920515E-5	7.551610488248979E-5
27	8.147790263637056E-4	3.974531835920515E-6	0.0	3.974531835920515E-5	7.94906367184103E-5
28	9.379895132772415E-4	3.974531835920515E-6	0.0	5.1668913866966693E-5	8.743970039025133E-5
29	0.001057225468354857	3.974531835920515E-6	0.0	5.1668913866966693E-5	9.141423222617185E-5
30	0.0011367161050732672	3.974531835920515E-6	0.0	5.1668913866966693E-5	1.0333782773393339E-4
31	0.0012082576781198367	3.974531835920515E-6	3.974531835920515E-6	5.1668913866966693E-5	1.0333782773393339E-4
32	0.0012837737830023264	3.974531835920515E-6	3.974531835920515E-6	5.1668913866966693E-5	1.1526142324169494E-4
33	0.0014626277156187496	3.974531835920515E-6	3.974531835920515E-6	5.1668913866966693E-5	1.1923595507761546E-4
34	0.0016136599253837292	3.974531835920515E-6	3.974531835920515E-6	5.564344570288721E-5	1.1923595507761546E-4
35	0.0017646921351487086	3.974531835920515E-6	3.974531835920515E-6	9.141423222617185E-5	1.2718501874945648E-4
36	0.0018839280902263242	3.974531835920515E-6	3.974531835920515E-6	9.538876406209237E-5	1.351340824212975E-4
37	0.0020270112363194627	7.94906367184103E-6	3.974531835920515E-6	1.0333782773393339E-4	1.4308314609313855E-4
38	0.002170094382412601	7.94906367184103E-6	3.974531835920515E-6	1.0333782773393339E-4	1.4705767792905905E-4
39	0.0022893303374902168	7.94906367184103E-6	3.974531835920515E-6	1.0333782773393339E-4	1.5103220976497958E-4
40	0.0025238277158095273	7.94906367184103E-6	3.974531835920515E-6	1.1128689140577442E-4	1.5103220976497958E-4
41	0.002722554307605553	7.94906367184103E-6	3.974531835920515E-6	1.1128689140577442E-4	1.6693033710866163E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	611985	0.0	46.109127	1
AGCAGTG	624050	0.0	45.204502	2
AGTGGTA	624775	0.0	45.12346	5
GTGGTAT	626075	0.0	45.11197	6
TCAACGC	629525	0.0	45.044296	12
TGGTATC	624705	0.0	45.039734	7
GTATCAA	626920	0.0	45.022297	9
CAACGCA	630635	0.0	45.017178	13
GGTATCA	627120	0.0	45.005314	8
ACGCAGA	628780	0.0	44.969093	15
TATCAAC	628175	0.0	44.967888	10
AACGCAG	633890	0.0	44.96656	14
ATCAACG	631260	0.0	44.940964	11
CGCAGAG	630660	0.0	44.82647	16
GCAGTGG	629095	0.0	44.810604	3
CAGTGGT	629300	0.0	44.793022	4
AGAGTAC	623655	0.0	44.773045	19
CAGAGTA	628920	0.0	44.621677	18
GAGTACT	186630	0.0	44.549644	20
GCAGAGT	633455	0.0	44.495502	17
>>END_MODULE