FastQCFastQC Report
Thu 26 May 2016
SRR805206_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805206_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22580394
Sequences flagged as poor quality0
Sequence length53
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3488331.5448490402780395No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCC268210.11878003545908013No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1666000.044.0307581
TCAACGC1704500.042.85837612
CAACGCA1698500.042.80638513
GTGGTAT1721550.042.608646
AACGCAG1719600.042.5749614
ACGCAGA1714150.042.52935815
GGTATCA1716700.042.4620748
GTATCAA1717050.042.461639
ATCAACG1720950.042.4309511
AGTGGTA1728000.042.3897555
TGGTATC1723100.042.267547
TATCAAC1725850.042.25329210
CGCAGAG1734150.042.02937716
AGAGTAC1712300.041.97144319
AGCAGTG1773250.041.5002142
CAGTGGT1771800.041.386954
GCAGTGG1779400.041.310553
CAGAGTA1741850.041.2202818
GAGTACT613000.040.908420
GCAGAGT1766850.040.84851517
Produced by FastQC (version 0.11.5)