##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805206_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	22580394
Sequences flagged as poor quality	0
Sequence length	53
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	34.6142844540268	35.0	35.0	35.0	33.0	37.0
2	35.60089110934025	36.0	35.0	37.0	35.0	37.0
3	38.08075120389839	39.0	39.0	39.0	35.0	39.0
4	38.03125893197435	39.0	39.0	39.0	35.0	39.0
5	37.93683453884817	39.0	39.0	39.0	35.0	39.0
6	37.99980748785872	39.0	39.0	39.0	35.0	39.0
7	38.00124705529939	39.0	39.0	39.0	35.0	39.0
8	38.98741266427858	40.0	40.0	40.0	37.0	40.0
9	38.96829771880863	40.0	40.0	40.0	37.0	40.0
10	38.87528835856451	40.0	39.0	40.0	36.0	40.0
11	38.848985008853255	40.0	39.0	40.0	37.0	40.0
12	38.94104079849094	40.0	40.0	40.0	37.0	40.0
13	38.882947082322836	40.0	40.0	40.0	37.0	40.0
14	39.020948837296636	40.0	40.0	40.0	37.0	40.0
15	39.037494474188534	40.0	40.0	40.0	37.0	40.0
16	39.0183963574772	40.0	40.0	40.0	37.0	40.0
17	39.012510632011114	40.0	40.0	40.0	37.0	40.0
18	38.88993194715734	40.0	40.0	40.0	37.0	40.0
19	38.786944860218114	40.0	40.0	40.0	37.0	40.0
20	38.8681372875956	40.0	40.0	40.0	37.0	40.0
21	38.77141032171538	40.0	40.0	40.0	37.0	40.0
22	38.813472962429266	40.0	40.0	40.0	37.0	40.0
23	38.50354240940171	40.0	39.0	40.0	36.0	40.0
24	38.65685492467492	40.0	39.0	40.0	36.0	40.0
25	38.72306856115974	40.0	39.0	40.0	36.0	40.0
26	38.699231156019685	40.0	39.0	40.0	36.0	40.0
27	38.16780592933852	40.0	39.0	40.0	35.0	40.0
28	38.406241715711424	40.0	39.0	40.0	35.0	40.0
29	38.39924471645623	40.0	39.0	40.0	35.0	40.0
30	38.41888113201213	40.0	39.0	40.0	35.0	40.0
31	38.2860197213565	40.0	39.0	40.0	35.0	40.0
32	38.30911245392795	40.0	39.0	40.0	35.0	40.0
33	38.30142016122482	40.0	38.0	40.0	35.0	40.0
34	38.29792363233343	40.0	38.0	40.0	35.0	40.0
35	38.100865910488544	40.0	38.0	40.0	35.0	40.0
36	38.0447798652229	40.0	38.0	40.0	35.0	40.0
37	38.14625998111459	40.0	38.0	40.0	35.0	40.0
38	38.20017954513991	40.0	38.0	40.0	35.0	40.0
39	38.17262130147065	40.0	38.0	40.0	35.0	40.0
40	38.08471707801024	40.0	38.0	40.0	35.0	40.0
41	38.007541985317	40.0	38.0	40.0	34.0	40.0
42	37.88113617503751	40.0	38.0	40.0	34.0	40.0
43	37.87974775816578	40.0	38.0	40.0	34.0	40.0
44	37.879561180376214	40.0	38.0	40.0	34.0	40.0
45	37.751645387587125	40.0	37.0	40.0	34.0	40.0
46	37.65880179061534	40.0	37.0	40.0	34.0	40.0
47	37.49166232440408	40.0	37.0	40.0	33.0	40.0
48	37.43380642516689	40.0	37.0	40.0	33.0	40.0
49	37.29656471007547	40.0	37.0	40.0	33.0	40.0
50	37.25977425371763	40.0	36.0	40.0	33.0	40.0
51	37.12195991797132	39.0	36.0	40.0	33.0	40.0
52	36.99928163343828	39.0	36.0	40.0	33.0	40.0
53	36.791364225088365	39.0	35.0	40.0	32.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	3.0
12	13.0
13	70.0
14	229.0
15	651.0
16	1480.0
17	2666.0
18	4334.0
19	6969.0
20	10661.0
21	15753.0
22	22389.0
23	29949.0
24	39039.0
25	50533.0
26	63987.0
27	81526.0
28	102064.0
29	128420.0
30	160571.0
31	202449.0
32	259270.0
33	339477.0
34	470930.0
35	697577.0
36	1120978.0
37	2108060.0
38	4161659.0
39	1.2498687E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	12.545197395581317	44.101396104957246	22.760360160234583	20.593046339226852
2	28.055285483503962	41.53530270552409	16.75018159559129	13.659230215380653
3	33.49659443497753	25.078415372202983	21.4086211250344	20.01636906778509
4	21.876199325839927	25.989519934860304	21.349906471959702	30.784374267340063
5	20.377540799332376	33.247032801996276	25.172930994915323	21.202495403756018
6	28.638207110115083	24.027215822717707	23.99742891997367	23.337148147193535
7	23.44714179920864	24.397882517018967	30.577787969510183	21.577187714262205
8	27.836369905680126	25.256968501081072	25.168648518710523	21.738013074528283
9	28.552575300501843	24.360925677381893	25.35358771861997	21.732911303496298
10	21.930990220985517	25.52987339370606	30.92437625313358	21.614760132174844
11	21.771391588649873	32.00675772087945	24.681442671018054	21.540408019452627
12	21.639547122162707	24.840487725767762	31.312070993978224	22.207894158091307
13	21.985192109579664	25.58446500092071	24.155326961965322	28.2750159275343
14	21.90354605858516	32.4193678817119	24.22732747710248	21.449758582600463
15	21.82189115034928	31.785592403746364	24.16815667609697	22.224359769807382
16	22.20192880602526	24.953284694678047	24.232464677099966	28.612321822196723
17	28.904668359639786	24.762783147185118	24.366027448413877	21.96652104476122
18	22.183107168103444	24.722566842722056	24.13473387576851	28.95959211340599
19	22.48839413519534	31.773170122717964	24.01537369099937	21.72306205108733
20	29.077800856796387	25.12202842873335	24.243899375715056	21.556271338755206
21	22.715812664739154	31.37908045360059	24.057636018220055	21.847470863440204
22	28.78062712280397	24.84064715611251	24.499346645589977	21.879379075493546
23	22.168023286041866	25.020604157748533	31.223002574711494	21.588369981498108
24	22.211388339813734	31.241288349530127	24.156376545068255	22.390946765587884
25	21.94738497477059	25.02722937429701	24.1917169381544	28.833668712778
26	22.129755574681294	29.174300501576724	26.967687100588233	21.72825682315375
27	22.773446734366104	24.46346153215927	30.694442267039275	22.06864946643535
28	26.77430694964844	24.750861300294407	26.53090995666417	21.943921793392978
29	27.008115978844298	24.743137785815428	26.518022670463587	21.73072356487668
30	26.88523061200792	24.37123993496305	26.54762357113875	22.19590588189028
31	25.246649814879223	25.48579533200351	26.72696499449921	22.540589858618056
32	23.863755433142575	26.22294810267704	27.345842592472035	22.567453871708352
33	23.704205515634495	25.58254740816303	27.411939756232773	23.301307319969705
34	23.38298879992971	25.775214551172137	27.6405938709484	23.201202777949756
35	23.593281853275013	25.802508140469115	27.667767887486818	22.93644211876905
36	23.71623807804239	25.509329022336814	27.58615726545781	23.188275634162984
37	23.481339608157413	25.7619198318683	27.6569797674921	23.099760792482186
38	23.620522299123746	25.73981215739637	27.815249813621502	22.824415729858387
39	23.711920172872095	25.388781967223423	27.70346257022796	23.195835289676523
40	23.48458135850065	25.801967848745242	27.46355975896612	23.249891033787982
41	23.613945797402828	25.96780197900887	27.523301852040316	22.89495037154799
42	23.92640270138776	25.58067853023291	27.4160008014032	23.07691796697613
43	23.577077530179498	25.855598445270704	27.605377479241504	22.961946545308287
44	23.542113569851793	25.778323442894752	27.6155013061331	23.064061681120357
45	23.77857091421877	25.31206939967478	27.466540220688795	23.442819465417656
46	23.625203351190418	25.664029600192094	27.32651609179184	23.384250956825642
47	23.774456725600093	25.710494688445205	27.394273988310392	23.12077459764431
48	23.870438221759994	25.323229523807246	27.401156064858746	23.405176189574018
49	23.549066504331147	25.888728956633795	27.296104753530877	23.266099785504185
50	23.646863734972914	25.796777505299506	27.494825821019774	23.061532938707803
51	23.757406535953272	25.45856817201684	27.32629023213678	23.45773505989311
52	23.686632748746543	25.85187840389322	27.083548675014264	23.377940172345973
53	23.743119805615436	25.874030364572025	27.049948729858304	23.332901099954235
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	6726.0
1	4203.5
2	1681.0
3	1349.0
4	1017.0
5	1001.0
6	985.0
7	1132.5
8	1609.0
9	1938.0
10	3303.0
11	4668.0
12	6753.0
13	8838.0
14	11148.0
15	13458.0
16	19745.0
17	26032.0
18	36108.5
19	46185.0
20	64237.5
21	82290.0
22	307447.5
23	532605.0
24	394096.5
25	269899.0
26	284210.0
27	315793.5
28	347377.0
29	383213.5
30	419050.0
31	453871.5
32	488693.0
33	544158.0
34	599623.0
35	669176.5
36	738730.0
37	816344.0
38	893958.0
39	970129.0
40	1046300.0
41	1129829.0
42	1285219.0
43	1357080.0
44	1433962.5
45	1510845.0
46	1560193.5
47	1609542.0
48	1644543.5
49	1679545.0
50	1673877.0
51	1668209.0
52	1629401.5
53	1590594.0
54	1526425.0
55	1462256.0
56	1372397.0
57	1282538.0
58	1160226.0
59	915811.0
60	793708.0
61	690545.5
62	587383.0
63	498308.0
64	409233.0
65	333829.0
66	258425.0
67	206279.0
68	154133.0
69	118712.0
70	83291.0
71	61928.5
72	40566.0
73	30640.0
74	20714.0
75	15354.5
76	7293.5
77	4592.0
78	3438.0
79	2284.0
80	1864.5
81	1445.0
82	1150.5
83	856.0
84	593.0
85	330.0
86	231.0
87	132.0
88	98.5
89	65.0
90	61.0
91	57.0
92	46.0
93	27.5
94	20.0
95	13.5
96	7.0
97	7.0
98	7.0
99	5.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	2.2580394E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.577775217405204
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.85845281821395	30.006981787304294
2	15.7011459186825	13.684420150000504
3	5.6190572197628	7.345980373696835
4	2.824686735934858	4.923742545526211
5	1.5410881803612775	3.357859715699188
6	1.024666778875611	2.6791619137550415
7	0.742426465943013	2.2647305553822017
8	0.5329155760463925	1.8578620146242963
9	0.4077564155013559	1.599220567635567
>10	2.514724335195044	20.105846918560218
>50	0.1743833861475585	5.151187442635628
>100	0.05608291698198962	4.00036825628253
>500	0.0019174217024311014	0.5663160042261226
>1k	5.835561568288918E-4	0.38958565586114213
>5k	4.082708873038634E-5	0.12634659968407375
>10k+	7.144740527817328E-5	1.940389499126073
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	348833	1.5448490402780395	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCC	26821	0.11878003545908013	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.757206893732678E-5	0.0	0.0	4.428620687486675E-6	1.3285862062460027E-5
2	6.642931031230013E-5	0.0	0.0	8.85724137497335E-6	1.3285862062460027E-5
3	7.08579309997868E-5	0.0	0.0	8.85724137497335E-6	1.77144827499467E-5
4	1.0185827581219353E-4	0.0	0.0	1.3285862062460027E-5	3.100034481240673E-5
5	1.1957275856214024E-4	0.0	0.0	2.6571724124920054E-5	3.985758618738008E-5
6	1.2400137924962692E-4	0.0	0.0	2.6571724124920054E-5	4.4286206874866754E-5
7	1.3285862062460026E-4	0.0	0.0	3.100034481240673E-5	5.314344824984011E-5
8	1.3728724131208695E-4	4.428620687486675E-6	0.0	4.4286206874866754E-5	5.314344824984011E-5
9	1.3728724131208695E-4	4.428620687486675E-6	0.0	4.4286206874866754E-5	5.314344824984011E-5
10	1.5057310337454697E-4	4.428620687486675E-6	0.0	4.871482756235343E-5	5.314344824984011E-5
11	1.594303447495203E-4	4.428620687486675E-6	0.0	4.871482756235343E-5	6.200068962481346E-5
12	1.9928793093690038E-4	4.428620687486675E-6	0.0	4.871482756235343E-5	6.642931031230013E-5
13	2.2585965506182045E-4	4.428620687486675E-6	0.0	4.871482756235343E-5	7.528655168727348E-5
14	2.302882757493071E-4	4.428620687486675E-6	0.0	4.871482756235343E-5	8.414379306224684E-5
15	2.3914551712428048E-4	4.428620687486675E-6	0.0	4.871482756235343E-5	8.857241374973351E-5
16	2.3914551712428048E-4	4.428620687486675E-6	0.0	6.642931031230013E-5	9.742965512470686E-5
17	2.6128862056171387E-4	4.428620687486675E-6	0.0	6.642931031230013E-5	9.742965512470686E-5
18	2.834317239991472E-4	4.428620687486675E-6	0.0	7.08579309997868E-5	1.0185827581219353E-4
19	3.188606894990406E-4	4.428620687486675E-6	0.0	7.08579309997868E-5	1.1514413787465356E-4
20	3.3214655156150064E-4	4.428620687486675E-6	0.0	7.971517237476015E-5	1.3728724131208695E-4
21	3.41003792936474E-4	4.428620687486675E-6	0.0	7.971517237476015E-5	1.417158619995736E-4
22	3.8528999981134074E-4	4.428620687486675E-6	0.0	7.971517237476015E-5	1.5057310337454697E-4
23	4.118617239362608E-4	4.428620687486675E-6	0.0	7.971517237476015E-5	1.63858965437007E-4
24	4.118617239362608E-4	4.428620687486675E-6	0.0	7.971517237476015E-5	1.7271620681198033E-4
25	4.694337928735876E-4	4.428620687486675E-6	0.0	8.414379306224684E-5	1.7271620681198033E-4
26	4.915768963110209E-4	8.85724137497335E-6	0.0	8.857241374973351E-5	1.7271620681198033E-4
27	5.624348273108078E-4	8.85724137497335E-6	0.0	1.0185827581219353E-4	1.815734481869537E-4
28	6.332927583105946E-4	8.85724137497335E-6	0.0	1.0185827581219353E-4	1.9928793093690038E-4
29	7.085793099978681E-4	8.85724137497335E-6	0.0	1.0628689649968022E-4	1.9928793093690038E-4
30	7.794372409976549E-4	8.85724137497335E-6	0.0	1.0628689649968022E-4	1.9928793093690038E-4
31	8.59152413372415E-4	1.3285862062460027E-5	0.0	1.0628689649968022E-4	2.0371655162438706E-4
32	9.610106891846086E-4	1.3285862062460027E-5	0.0	1.0628689649968022E-4	2.1257379299936043E-4
33	0.0011071551718716689	1.3285862062460027E-5	0.0	1.0628689649968022E-4	2.1257379299936043E-4
34	0.001271014137308676	1.3285862062460027E-5	0.0	1.1071551718716689E-4	2.1257379299936043E-4
35	0.0013861582751833294	1.3285862062460027E-5	0.0	1.1514413787465356E-4	2.2585965506182045E-4
36	0.0014880165509955229	1.3285862062460027E-5	0.0	1.1957275856214024E-4	2.2585965506182045E-4
37	0.0016031606888701766	1.3285862062460027E-5	0.0	1.1957275856214024E-4	2.4357413781176713E-4
38	0.0017360193094947767	1.3285862062460027E-5	0.0	1.1957275856214024E-4	2.524313791867405E-4
39	0.001855592068056917	1.3285862062460027E-5	0.0	1.1957275856214024E-4	2.5685999987422716E-4
40	0.002006165171431464	1.3285862062460027E-5	0.0	1.2400137924962692E-4	2.6128862056171387E-4
41	0.002178881378243444	1.3285862062460027E-5	0.0	1.2400137924962692E-4	2.7900310331166055E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	166600	0.0	44.030758	1
TCAACGC	170450	0.0	42.858376	12
CAACGCA	169850	0.0	42.806385	13
GTGGTAT	172155	0.0	42.60864	6
AACGCAG	171960	0.0	42.57496	14
ACGCAGA	171415	0.0	42.529358	15
GGTATCA	171670	0.0	42.462074	8
GTATCAA	171705	0.0	42.46163	9
ATCAACG	172095	0.0	42.43095	11
AGTGGTA	172800	0.0	42.389755	5
TGGTATC	172310	0.0	42.26754	7
TATCAAC	172585	0.0	42.253292	10
CGCAGAG	173415	0.0	42.029377	16
AGAGTAC	171230	0.0	41.971443	19
AGCAGTG	177325	0.0	41.500214	2
CAGTGGT	177180	0.0	41.38695	4
GCAGTGG	177940	0.0	41.31055	3
CAGAGTA	174185	0.0	41.22028	18
GAGTACT	61300	0.0	40.9084	20
GCAGAGT	176685	0.0	40.848515	17
>>END_MODULE