FastQCFastQC Report
Thu 26 May 2016
SRR805205_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805205_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27141814
Sequences flagged as poor quality0
Sequence length53
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1840730.6781897481133723No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA435990.16063406815771414No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT655550.037.919761
AGCAGTG780450.031.8361842
TCAACGC822200.030.02523612
CAACGCA823050.029.97424313
AGTGGTA832550.029.7705255
ATCAACG830650.029.74808911
GTGGTAT837000.029.5954026
ACGCAGA837200.029.52096215
AACGCAG837150.029.51149714
GGTATCA841700.029.3659258
GTATCAA841750.029.35869
TATCAAC855800.028.90406610
CGCAGAG854850.028.88395716
GAGTACT399050.028.85603120
TGGTATC858500.028.8213757
GCAGTGG865300.028.7551143
AGAGTAC855150.028.59352119
CAGTGGT892800.027.8509754
GTACTTT414500.027.67840822
CAGAGTA898250.027.34711818
Produced by FastQC (version 0.11.5)