##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805205_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	27141814
Sequences flagged as poor quality	0
Sequence length	53
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.617766299629054	37.0	35.0	37.0	32.0	37.0
2	35.979714030904496	37.0	35.0	37.0	35.0	37.0
3	37.94221189490135	39.0	38.0	39.0	35.0	39.0
4	37.942010360840285	39.0	38.0	39.0	35.0	39.0
5	37.90469723210099	39.0	38.0	39.0	35.0	39.0
6	37.88952109096319	39.0	38.0	39.0	35.0	39.0
7	37.85679682279158	39.0	38.0	39.0	35.0	39.0
8	38.837856563308556	40.0	39.0	40.0	36.0	40.0
9	38.84999808782125	40.0	39.0	40.0	36.0	40.0
10	38.817176331692494	40.0	39.0	40.0	36.0	40.0
11	38.831090213793374	40.0	39.0	40.0	36.0	40.0
12	38.81612367544778	40.0	39.0	40.0	36.0	40.0
13	38.90315422543239	40.0	40.0	40.0	37.0	40.0
14	38.89698529361376	40.0	40.0	40.0	37.0	40.0
15	38.87837187300746	40.0	39.0	40.0	37.0	40.0
16	38.84678717494711	40.0	39.0	40.0	36.0	40.0
17	38.868764519571165	40.0	39.0	40.0	37.0	40.0
18	38.80063303801286	40.0	39.0	40.0	36.0	40.0
19	38.79023797009293	40.0	39.0	40.0	36.0	40.0
20	38.71136984432949	40.0	39.0	40.0	36.0	40.0
21	38.699471265995705	40.0	39.0	40.0	36.0	40.0
22	38.607230489458075	40.0	39.0	40.0	36.0	40.0
23	38.551348299712025	40.0	39.0	40.0	36.0	40.0
24	38.51314912113096	40.0	39.0	40.0	36.0	40.0
25	38.460358950216076	40.0	39.0	40.0	35.0	40.0
26	38.46450627802548	40.0	39.0	40.0	35.0	40.0
27	38.429403944776865	40.0	39.0	40.0	35.0	40.0
28	38.36176052934413	40.0	39.0	40.0	35.0	40.0
29	38.30807163441618	40.0	38.0	40.0	35.0	40.0
30	38.27864541404639	40.0	38.0	40.0	35.0	40.0
31	38.31278200491684	40.0	39.0	40.0	35.0	40.0
32	38.3146583349219	40.0	39.0	40.0	35.0	40.0
33	38.24659471912968	40.0	38.0	40.0	35.0	40.0
34	38.24907708821525	40.0	38.0	40.0	35.0	40.0
35	38.19053715422263	40.0	38.0	40.0	35.0	40.0
36	38.115510812947136	40.0	38.0	40.0	34.0	40.0
37	38.0630445702708	40.0	38.0	40.0	34.0	40.0
38	38.00507445817733	40.0	38.0	40.0	34.0	40.0
39	37.929882873709175	40.0	38.0	40.0	34.0	40.0
40	37.839114548497015	40.0	38.0	40.0	33.0	40.0
41	37.78229078572272	40.0	38.0	40.0	33.0	40.0
42	37.71694412171567	40.0	38.0	40.0	33.0	40.0
43	37.64575599110656	40.0	37.0	40.0	33.0	40.0
44	37.581355874003116	40.0	37.0	40.0	33.0	40.0
45	37.52452628995247	40.0	37.0	40.0	33.0	40.0
46	37.46065764064259	40.0	37.0	40.0	33.0	40.0
47	37.51973604269781	40.0	37.0	40.0	33.0	40.0
48	37.57467319612462	40.0	37.0	40.0	33.0	40.0
49	37.5754232933731	40.0	37.0	40.0	33.0	40.0
50	37.46742321644383	40.0	37.0	40.0	33.0	40.0
51	37.34106077066183	40.0	37.0	40.0	33.0	40.0
52	37.20638473169111	40.0	36.0	40.0	33.0	40.0
53	36.89604504695228	39.0	36.0	40.0	32.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	5.0
11	6.0
12	28.0
13	89.0
14	254.0
15	775.0
16	1787.0
17	3574.0
18	6239.0
19	10266.0
20	15582.0
21	22362.0
22	31692.0
23	42966.0
24	57121.0
25	73383.0
26	94166.0
27	118599.0
28	148288.0
29	184819.0
30	229256.0
31	284024.0
32	355000.0
33	450823.0
34	598844.0
35	840936.0
36	1259883.0
37	2120279.0
38	4423287.0
39	1.576748E7
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	21.635480959378764	29.367609696242113	21.73174571161677	27.265163632762352
2	24.05455287550051	26.559890212201736	25.81354731853958	23.572009593758178
3	23.276572450168583	26.10182945030866	25.634362537448673	24.987235562074076
4	22.67190763299756	26.355327613695973	26.186145848615716	24.786618904690748
5	25.030902503421476	27.932911926962582	25.464267053042217	21.571918516573728
6	25.735159042796475	25.47319792258542	26.06749865723787	22.724144377380227
7	24.06702440743275	26.118751679604024	27.696737587251906	22.117486325711315
8	24.633976196285186	26.0915022113113	26.266085236602095	23.00843635580142
9	24.180631405108	25.848725512598385	27.134590193566282	22.836052888727334
10	22.59685001157255	26.278833094943472	28.24596027369431	22.87835661978967
11	22.50723919926649	28.113459918338545	26.47303529528277	22.906265587112195
12	22.55844064070294	26.227782711944016	27.991677343305057	23.22209930404799
13	22.662000410142078	26.524045150408888	25.90792568249123	24.906028756957806
14	22.94501023402489	28.300418682406413	25.912608494038018	22.841962589530677
15	23.01450079939388	27.774473732669453	25.92006930708463	23.290956160852033
16	23.16050798962811	26.093952305472286	25.81208094639511	24.933458758504496
17	24.99950445463962	26.080482314115038	25.888295454386355	23.031717776858983
18	23.034481040950322	26.214519044305582	25.900004325429393	24.8509955893147
19	23.421297485864432	27.933858805457884	25.72408388031839	22.920759828359298
20	25.09115271366903	26.079391745886994	25.92963388519279	22.89982165525119
21	23.226428417791087	27.746365810332353	25.995506416778184	23.03169935509837
22	24.878698232918403	25.924203150165276	26.01221126929836	23.18488734761796
23	22.992512585931067	26.228766433960534	27.69816343152304	23.080557548585368
24	22.96327356749258	27.749291185917052	25.924693168997475	23.3627420775929
25	23.17724600131738	25.965025771674654	25.964395747461833	24.893332479546135
26	23.410329169597876	26.806734435657102	26.955630157954808	22.827306236790214
27	23.54881659715154	25.6540185560184	27.777509638817804	23.019655208012257
28	24.56035178783555	25.789514289649173	26.635194685218906	23.01493923729637
29	24.550407721458853	25.851551410675793	26.677059978378747	22.920980889486607
30	24.318065107954833	25.720292681985075	26.74644369753621	23.215198512523884
31	24.02068631079706	25.988815633325025	26.72023690089395	23.270261154983967
32	23.727452409776294	26.175280694208574	26.87357963620265	23.223687259812483
33	23.588353379770417	25.989150909368107	26.955921221772428	23.466574489089048
34	23.592188790329192	26.079358586717895	26.928115416309318	23.400337206643595
35	23.550743513311232	26.136226561717653	26.9930042258782	23.32002569909292
36	23.513863148572163	26.040164448846344	27.00079663061577	23.445175771965722
37	23.700129254441133	26.084417202181108	26.80601230264123	23.409441240736527
38	23.68562764448979	26.171378965311604	26.888353888211007	23.254639501987597
39	23.660640368399843	26.053428116484774	26.873513317864457	23.41241819725093
40	23.739415501115733	26.115104171003455	26.706483214423326	23.438997113457486
41	23.645040821516204	26.239808437269517	26.814589474380746	23.300561266833526
42	23.615776012612862	26.072914654856895	26.86914367624802	23.44216565628222
43	23.70630791294937	26.097190850987335	26.740202405041902	23.456298831021392
44	23.64420447358456	26.11148245286774	26.853573604181356	23.390739469366345
45	23.600692275026276	25.955037493072496	26.916594447224497	23.527675784676735
46	23.673303486642418	26.094626541910575	26.720900084275872	23.51116988717114
47	23.581290476752955	26.179473486923165	26.84697124517912	23.39226479114476
48	23.562363959903344	26.042986662571632	26.843375317508254	23.55127406001677
49	23.65264532429557	26.175763344336527	26.660690401901654	23.510900929466246
50	23.671494469750623	26.227112159857853	26.74040136005648	23.36099201033505
51	23.67105971620025	26.075309483736053	26.732940547009864	23.52069025305383
52	23.75827938397927	26.179396115528608	26.557067998476448	23.505256502015673
53	23.70543840584863	26.272672858195843	26.56904951157649	23.452839224379034
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	48214.0
1	28584.5
2	8955.0
3	7510.5
4	6066.0
5	6155.5
6	6245.0
7	6655.5
8	7972.0
9	8878.0
10	11329.5
11	13781.0
12	18492.0
13	23203.0
14	26997.5
15	30792.0
16	40208.0
17	49624.0
18	64489.0
19	79354.0
20	104942.0
21	130530.0
22	280068.5
23	429607.0
24	356235.5
25	312250.0
26	341636.0
27	380032.5
28	418429.0
29	466441.0
30	514453.0
31	559426.0
32	604399.0
33	664576.0
34	724753.0
35	798922.5
36	873092.0
37	961949.0
38	1050806.0
39	1136868.0
40	1222930.0
41	1316604.5
42	1496548.0
43	1582817.0
44	1668093.5
45	1753370.0
46	1822941.0
47	1892512.0
48	1926792.5
49	1961073.0
50	1969755.5
51	1978438.0
52	1945219.5
53	1912001.0
54	1839838.5
55	1767676.0
56	1661425.0
57	1555174.0
58	1422873.5
59	1142591.5
60	994610.0
61	875894.5
62	757179.0
63	651441.5
64	545704.0
65	454019.5
66	362335.0
67	294355.0
68	226375.0
69	179251.0
70	132127.0
71	100226.5
72	68326.0
73	51894.5
74	35463.0
75	27622.5
76	14856.0
77	9930.0
78	7316.0
79	4702.0
80	3790.0
81	2878.0
82	2226.5
83	1575.0
84	1074.5
85	574.0
86	416.0
87	258.0
88	212.0
89	166.0
90	148.5
91	131.0
92	99.0
93	46.0
94	25.0
95	16.5
96	8.0
97	7.5
98	7.0
99	4.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	2.7141814E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.04716210326304
#Duplication Level	Percentage of deduplicated	Percentage of total
1	42.12804594724447	11.815721337760793
2	19.873873396573856	11.148114975468678
3	11.782947529454079	9.914347182385237
4	7.553293856659041	8.473938288451878
5	4.961259874307257	6.957462996555503
6	3.269118876477232	5.501370423803643
7	2.2631004182821384	4.443148100126507
8	1.6054610409498822	3.602290085279581
9	1.0906993208670528	2.753191859244942
>10	5.12732571924773	25.26819575355237
>50	0.2671286301120243	5.023085532691011
>100	0.07508904704945817	3.3926749046876954
>500	0.0021754845189096857	0.3908359951250998
>1k	3.4421779754916686E-4	0.14744887118901237
>5k	8.198427557632254E-5	0.14250694288882954
>10k+	5.465618369816334E-5	1.0256667507892359
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	184073	0.6781897481133723	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	43599	0.16063406815771414	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.473740848714091E-4	0.0	0.0	0.0	2.947481697428182E-5
2	1.5105843699319435E-4	0.0	0.0	0.0	3.315916909606705E-5
3	1.5105843699319435E-4	0.0	0.0	0.0	4.0527873339637506E-5
4	1.5105843699319435E-4	0.0	0.0	0.0	6.263398607034888E-5
5	1.5474278911497955E-4	0.0	0.0	0.0	8.474009880106023E-5
6	1.584271412367648E-4	0.0	0.0	0.0	1.0316185940998638E-4
7	1.584271412367648E-4	0.0	0.0	0.0	1.1421491577534206E-4
8	1.584271412367648E-4	0.0	0.0	0.0	1.2158362001891252E-4
9	1.584271412367648E-4	0.0	0.0	0.0	1.4000538062783867E-4
10	1.584271412367648E-4	0.0	0.0	0.0	1.584271412367648E-4
11	1.584271412367648E-4	3.6843521217852276E-6	0.0	0.0	1.8053325396747617E-4
12	1.6579584548033526E-4	3.6843521217852276E-6	0.0	0.0	2.1000807094175797E-4
13	1.6948019760212047E-4	3.6843521217852276E-6	0.0	0.0	2.505359442813955E-4
14	1.7684890184569094E-4	3.6843521217852276E-6	0.0	0.0	2.91063817621033E-4
15	1.8421760608926138E-4	3.6843521217852276E-6	0.0	0.0	3.1316993035174434E-4
16	1.9527066245461706E-4	3.6843521217852276E-6	0.0	0.0	3.4264474732602617E-4
17	2.1000807094175797E-4	3.6843521217852276E-6	0.0	0.0	3.4264474732602617E-4
18	2.136924230635432E-4	3.6843521217852276E-6	0.0	0.0	3.5001345156959664E-4
19	2.136924230635432E-4	3.6843521217852276E-6	0.0	0.0	3.8685697278744894E-4
20	2.247454794288989E-4	3.6843521217852276E-6	0.0	0.0	3.9422567703101936E-4
21	2.4316724003782504E-4	3.6843521217852276E-6	0.0	0.0	4.015943812745898E-4
22	2.6158900064675116E-4	3.6843521217852276E-6	0.0	0.0	4.1264743763994553E-4
23	2.8737946549924775E-4	3.6843521217852276E-6	0.0	0.0	4.384379024924421E-4
24	2.9843252186460346E-4	7.368704243570455E-6	0.0	0.0	4.384379024924421E-4
25	3.2422298671710005E-4	7.368704243570455E-6	0.0	0.0	4.494909588577978E-4
26	3.463290994478114E-4	7.368704243570455E-6	0.0	0.0	4.494909588577978E-4
27	3.7948826854387847E-4	7.368704243570455E-6	0.0	0.0	4.494909588577978E-4
28	4.1264743763994553E-4	7.368704243570455E-6	0.0	0.0	4.7159707158850913E-4
29	4.789657758320796E-4	7.368704243570455E-6	0.0	0.0	5.01071888562791E-4
30	5.158092970499318E-4	7.368704243570455E-6	0.0	0.0	5.047562406845762E-4
31	5.858119873638513E-4	7.368704243570455E-6	0.0	0.0	5.121249449281467E-4
32	6.263398607034887E-4	7.368704243570455E-6	0.0	0.0	5.379154097806433E-4
33	7.184486637481194E-4	7.368704243570455E-6	0.0	0.0	5.526528182677842E-4
34	7.995044104273945E-4	7.368704243570455E-6	0.0	0.0	5.526528182677842E-4
35	8.695071007413138E-4	1.1053056365355684E-5	1.842176060892614E-5	0.0	6.116024522163479E-4
36	9.137193262027365E-4	1.1053056365355684E-5	1.842176060892614E-5	0.0	6.30024212825274E-4
37	9.83722016516656E-4	1.473740848714091E-5	1.842176060892614E-5	0.0	6.410772691906296E-4
38	0.0010831995238048569	1.473740848714091E-5	1.842176060892614E-5	0.0	6.558146776777706E-4
39	0.0012232049044326957	1.473740848714091E-5	1.842176060892614E-5	0.0	6.63183381921341E-4
40	0.0013116293553555411	1.473740848714091E-5	1.842176060892614E-5	0.0	6.779207904084819E-4
41	0.0014921626093230172	1.473740848714091E-5	1.842176060892614E-5	0.0	7.110799595045489E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	65555	0.0	37.91976	1
AGCAGTG	78045	0.0	31.836184	2
TCAACGC	82220	0.0	30.025236	12
CAACGCA	82305	0.0	29.974243	13
AGTGGTA	83255	0.0	29.770525	5
ATCAACG	83065	0.0	29.748089	11
GTGGTAT	83700	0.0	29.595402	6
ACGCAGA	83720	0.0	29.520962	15
AACGCAG	83715	0.0	29.511497	14
GGTATCA	84170	0.0	29.365925	8
GTATCAA	84175	0.0	29.3586	9
TATCAAC	85580	0.0	28.904066	10
CGCAGAG	85485	0.0	28.883957	16
GAGTACT	39905	0.0	28.856031	20
TGGTATC	85850	0.0	28.821375	7
GCAGTGG	86530	0.0	28.755114	3
AGAGTAC	85515	0.0	28.593521	19
CAGTGGT	89280	0.0	27.850975	4
GTACTTT	41450	0.0	27.678408	22
CAGAGTA	89825	0.0	27.347118	18
>>END_MODULE