FastQCFastQC Report
Thu 26 May 2016
SRR805204_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805204_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18931474
Sequences flagged as poor quality0
Sequence length53
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT374010.1975598941741145No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT244200.031.9107281
AGCAGTG350600.022.3203092
TCAACGC350250.022.2688112
CAACGCA351800.022.24417513
AACGCAG359550.021.75163714
ACGCAGA359000.021.74568215
AGTGGTA362900.021.6479765
GGTATCA362950.021.5737728
GTGGTAT364650.021.4925296
ATCAACG364750.021.4028811
GCAGTGG366850.021.389263
GTATCAA366250.021.3793879
TGGTATC370200.021.1195327
TATCAAC375400.020.89584510
CGCAGAG374850.020.84500716
AGAGTAC380300.020.29910919
CAGTGGT392950.019.9566124
GAGTACT154800.019.8414120
CAGAGTA407500.019.0768118
GCAGAGT415400.018.82149917
Produced by FastQC (version 0.11.5)