FastQCFastQC Report
Thu 26 May 2016
SRR805203_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805203_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18735173
Sequences flagged as poor quality0
Sequence length53
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT970200.5178495015765266No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT731150.041.873491
AGCAGTG834400.036.8778762
CAACGCA846200.036.01648313
TCAACGC844350.035.9423212
AGTGGTA855400.035.9093365
GTGGTAT855350.035.7108766
AACGCAG855700.035.6440914
GTATCAA860200.035.6024179
GGTATCA861650.035.539788
ACGCAGA858050.035.52729815
ATCAACG861450.035.37071211
TATCAAC868700.035.26758210
TGGTATC867650.035.2181747
AGAGTAC869350.034.85736519
CGCAGAG875300.034.83788316
GCAGTGG882700.034.8173753
CAGTGGT893650.034.3960154
CAGAGTA887000.034.19819318
GCAGAGT906200.033.445117
GTACATG688350.033.1324922
Produced by FastQC (version 0.11.5)