##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805203_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	18735173
Sequences flagged as poor quality	0
Sequence length	53
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.18264581810907	37.0	37.0	37.0	35.0	37.0
2	36.16470160163453	37.0	37.0	37.0	35.0	37.0
3	37.93289146569396	39.0	38.0	39.0	35.0	39.0
4	37.87500323589219	39.0	38.0	39.0	35.0	39.0
5	37.84018765132299	39.0	38.0	39.0	35.0	39.0
6	37.812736503687475	39.0	38.0	39.0	35.0	39.0
7	37.73760023459618	39.0	38.0	39.0	35.0	39.0
8	38.66813885305463	40.0	39.0	40.0	36.0	40.0
9	38.54697877623014	40.0	39.0	40.0	36.0	40.0
10	38.538029139095755	40.0	38.0	40.0	36.0	40.0
11	38.54596789685369	40.0	38.0	40.0	36.0	40.0
12	38.37498949169031	40.0	38.0	40.0	35.0	40.0
13	38.5659307229242	40.0	39.0	40.0	36.0	40.0
14	38.57589508247402	40.0	39.0	40.0	36.0	40.0
15	38.51743002319754	40.0	38.0	40.0	36.0	40.0
16	38.48644861726123	40.0	38.0	40.0	36.0	40.0
17	38.039092406565985	40.0	38.0	40.0	34.0	40.0
18	38.14673139127138	40.0	38.0	40.0	34.0	40.0
19	38.24899631297774	40.0	38.0	40.0	34.0	40.0
20	38.17449841536024	40.0	38.0	40.0	35.0	40.0
21	38.1631097828667	40.0	38.0	40.0	35.0	40.0
22	38.07522359147684	40.0	38.0	40.0	34.0	40.0
23	37.75815264689576	40.0	38.0	40.0	34.0	40.0
24	37.7072484465449	40.0	38.0	40.0	34.0	40.0
25	37.66584525266994	40.0	38.0	40.0	34.0	40.0
26	37.4250314635472	39.0	37.0	40.0	33.0	40.0
27	37.398865598945896	39.0	37.0	40.0	33.0	40.0
28	37.3263019241936	39.0	37.0	40.0	33.0	40.0
29	37.10817423463344	39.0	37.0	40.0	33.0	40.0
30	37.01397969476983	38.0	36.0	40.0	32.0	40.0
31	36.87871726618164	38.0	36.0	40.0	32.0	40.0
32	36.684498830088195	38.0	36.0	40.0	31.0	40.0
33	36.59332337096647	38.0	36.0	40.0	31.0	40.0
34	36.48390927588446	38.0	36.0	40.0	31.0	40.0
35	36.23108737773598	38.0	35.0	40.0	31.0	40.0
36	36.08036611137778	38.0	35.0	39.0	30.0	40.0
37	35.92188724384878	38.0	35.0	39.0	30.0	40.0
38	35.87265369794023	38.0	35.0	39.0	30.0	40.0
39	35.749888992218004	38.0	35.0	39.0	30.0	40.0
40	35.65482982196108	38.0	35.0	39.0	30.0	40.0
41	35.42192868995659	37.0	34.0	38.0	30.0	40.0
42	35.23656333464335	37.0	34.0	38.0	30.0	40.0
43	34.93140794589941	37.0	34.0	38.0	29.0	40.0
44	34.781788991219884	36.0	33.0	38.0	29.0	40.0
45	34.69029733539156	36.0	33.0	38.0	28.0	39.0
46	34.58218512313711	36.0	33.0	38.0	28.0	39.0
47	34.3844249530015	36.0	33.0	38.0	28.0	39.0
48	34.22227198008793	36.0	33.0	38.0	28.0	39.0
49	33.90281648319981	36.0	32.0	38.0	27.0	39.0
50	33.67139652246606	35.0	32.0	38.0	27.0	39.0
51	33.37922969806577	35.0	32.0	38.0	26.0	39.0
52	32.91391795528122	34.0	31.0	37.0	26.0	38.0
53	34.98904541740821	37.0	34.0	39.0	28.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	6.0
12	16.0
13	93.0
14	344.0
15	988.0
16	2118.0
17	3664.0
18	5849.0
19	9130.0
20	14924.0
21	22869.0
22	33378.0
23	46364.0
24	61837.0
25	80514.0
26	103842.0
27	131145.0
28	164216.0
29	203543.0
30	253230.0
31	316996.0
32	407650.0
33	542382.0
34	757553.0
35	1132454.0
36	1949045.0
37	4105683.0
38	7563127.0
39	822211.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	25.048928024310214	28.89840408732815	23.273129103211375	22.77953878515026
2	25.36777749530255	25.681983294202837	26.28118245825646	22.669056752238156
3	25.366779372680465	24.347445310486325	25.285370997107954	25.000404319725256
4	23.296235375034968	26.600437583362584	24.63517684090774	25.468150200694705
5	25.562368706176347	28.78602188514619	23.57171188117665	22.079897527500812
6	27.53375162321693	24.61653276433583	25.445220068157365	22.404495544289876
7	23.86849056584639	25.162826091864748	28.470753912974278	22.497929429314585
8	26.608299800594317	24.827654380346527	25.39684047753389	23.167205341525268
9	26.139811999600965	25.01561634899235	25.871808069239606	22.972763582167083
10	22.654479891912395	25.343107320119223	29.123280580328775	22.87913220763961
11	22.66450381856629	28.56295482299523	25.74643959786227	23.026101760576218
12	22.853314458318586	25.10889010739319	28.804463134661205	23.233332299627016
13	22.765559730886924	25.407659699752973	25.3254613661694	26.501319203190704
14	22.947484925813068	28.622057559863475	25.313019527495157	23.117437986828303
15	23.07988829353217	28.19752451712082	25.2611224887008	23.46146470064621
16	23.213706113095405	24.995952799581836	25.176410167122555	26.6139309202002
17	26.640885568550658	25.10226620271935	25.02917373647951	23.227674492250486
18	23.17380255842847	25.027358968075713	25.228648809381156	26.570189664114658
19	23.49640966752749	28.38765887029706	25.11073156356763	23.005199898607824
20	26.693246974554224	24.926030840494505	25.29556572549397	23.085156459457302
21	23.294271155115567	28.163337482925833	25.371951462631277	23.170439899327324
22	26.574491732742473	24.85333869081433	25.383875558555026	23.188294017888172
23	23.44014650945577	24.987455413408778	28.45427154582453	23.11812653131092
24	23.093146777988117	28.210398697679494	25.209764542873447	23.486689981458937
25	23.338332664448842	24.91771493116183	25.24784799158246	26.496104412806865
26	23.78145640822212	27.232697557690024	26.07125111681648	22.91459491727138
27	23.736145911222703	24.57843330296443	28.50936577954204	23.176055006270825
28	26.419772051210842	24.70227523386093	25.795790623337183	23.08216209159104
29	26.419291671339252	24.726134100816683	25.797215750289578	23.05735847755449
30	26.03887351347116	24.69018033620506	25.907580357010847	23.363365793312934
31	25.083985079828192	25.276174391344025	26.03747507428941	23.602365454538372
32	24.527582424779318	25.63907469656138	26.240574346444518	23.59276853221478
33	24.22487905502661	25.33938704489145	26.392395736084207	24.04333816399774
34	24.202749555608587	25.386256107696468	26.465920544208476	23.94507379248646
35	24.176851742975632	25.445310806577552	26.473115567174105	23.90472188327271
36	24.018112883185015	25.415409828347997	26.496099075252733	24.07037821321426
37	24.321291295255186	25.439652999201023	26.345030280745206	23.89402542479859
38	24.31400019631524	25.459754228050098	26.381715290272474	23.84453028536219
39	24.25922087829133	25.395965118656765	26.37820851720985	23.966605485842056
40	24.2644036433504	25.50353818456867	26.2171478213732	24.01491035070773
41	24.19104963695825	25.649098623215277	26.31423259342201	23.845619146404466
42	24.203219260371924	25.498937212909645	26.360060833171918	23.93778269354652
43	24.385592809844884	25.443079708951714	26.255802388373993	23.915525092829405
44	24.2368672015999	25.39151893606747	26.359318913148012	24.012294949184618
45	24.219039770809694	25.225131361210273	26.35430161226694	24.20152725571309
46	24.28402449232788	25.399893558495563	26.19118062053657	24.124901328639986
47	24.20056115841578	25.420309703038235	26.27789986246724	24.101229276078744
48	24.187073159132293	25.32068425522412	26.298006428870448	24.194236156773144
49	24.193029869540037	25.5924351485839	26.116796466197563	24.097738515678504
50	24.27362159933084	25.54741821706157	26.203729210293385	23.975230973314204
51	24.202706855175556	25.372314416312037	26.23276550475408	24.19221322375833
52	24.43676394127772	25.46218815273283	26.02844393270348	24.07260397328597
53	24.48638718201321	25.524786987555437	25.96524195426431	24.023583876167038
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1994.0
1	1442.0
2	890.0
3	922.0
4	954.0
5	1018.5
6	1083.0
7	1257.0
8	1716.0
9	2001.0
10	2796.5
11	3592.0
12	5342.5
13	7093.0
14	8974.5
15	10856.0
16	15622.0
17	20388.0
18	28357.0
19	36326.0
20	49151.0
21	61976.0
22	138667.0
23	215358.0
24	187042.0
25	179398.0
26	200070.0
27	225231.5
28	250393.0
29	278599.0
30	306805.0
31	334688.5
32	362572.0
33	403075.0
34	443578.0
35	495098.0
36	546618.0
37	608386.0
38	670154.0
39	729180.0
40	788206.0
41	858076.5
42	995925.5
43	1063904.0
44	1132435.0
45	1200966.0
46	1261030.0
47	1321094.0
48	1362074.5
49	1403055.0
50	1420105.5
51	1437156.0
52	1419153.0
53	1401150.0
54	1361134.0
55	1321118.0
56	1248854.0
57	1176590.0
58	1073145.0
59	864180.0
60	758660.0
61	671099.5
62	583539.0
63	505295.5
64	427052.0
65	354008.5
66	280965.0
67	227285.5
68	173606.0
69	138046.0
70	102486.0
71	75130.5
72	47775.0
73	35388.0
74	23001.0
75	17831.0
76	9385.5
77	6110.0
78	4387.5
79	2665.0
80	2198.0
81	1731.0
82	1233.5
83	736.0
84	480.0
85	224.0
86	162.5
87	101.0
88	79.0
89	57.0
90	47.5
91	38.0
92	27.0
93	9.5
94	3.0
95	2.0
96	1.0
97	1.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	1.8735173E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.104159196481405
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.23008454778784	34.26463326665617
2	14.90922322400125	14.343912949264679
3	5.307830557155892	7.65986178328127
4	2.479557024143569	4.771080233046238
5	1.431002871236831	3.4418594964299247
6	0.8854173974261406	2.555535566467279
7	0.6338305498604403	2.134291997185598
8	0.4637153548246381	1.7845309800269802
9	0.3440039081742207	1.489321688672204
>10	2.169361463503087	19.51593333459913
>50	0.11254496585576339	3.6137967349296725
>100	0.030685370914336003	2.6665946939765686
>500	0.0022746949647909688	0.7115940555155532
>1k	4.124099274986696E-4	0.32311394353451794
>5k	2.2264089485999396E-5	0.08618834716981245
>10k+	3.3396134228999094E-5	0.6377509292444122
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	97020	0.5178495015765266	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.963925339787361E-4	5.337554128803615E-6	0.0	0.0	1.6012662386410843E-5
2	5.017300881075397E-4	5.337554128803615E-6	0.0	0.0	2.135021651521446E-5
3	5.070676422363434E-4	5.337554128803615E-6	0.0	0.0	2.6687770644018073E-5
4	5.12405196365147E-4	5.337554128803615E-6	0.0	0.0	2.6687770644018073E-5
5	5.230803046227542E-4	1.067510825760723E-5	0.0	0.0	2.6687770644018073E-5
6	5.497680752667723E-4	1.067510825760723E-5	0.0	1.067510825760723E-5	3.2025324772821686E-5
7	5.551056293955759E-4	1.067510825760723E-5	0.0	1.067510825760723E-5	4.803798715923253E-5
8	5.657807376531831E-4	1.067510825760723E-5	0.0	1.067510825760723E-5	4.803798715923253E-5
9	5.817934000395939E-4	1.6012662386410843E-5	0.0	1.067510825760723E-5	6.938820367444698E-5
10	5.978060624260048E-4	1.6012662386410843E-5	0.0	1.067510825760723E-5	6.938820367444698E-5
11	6.191562789412192E-4	1.6012662386410843E-5	0.0	1.067510825760723E-5	6.938820367444698E-5
12	6.191562789412192E-4	1.6012662386410843E-5	0.0	1.067510825760723E-5	6.938820367444698E-5
13	6.298313871988265E-4	2.135021651521446E-5	0.0	1.6012662386410843E-5	7.47257578032506E-5
14	6.618567119716481E-4	2.135021651521446E-5	0.0	2.135021651521446E-5	8.006331193205421E-5
15	6.778693743580591E-4	2.135021651521446E-5	0.0	2.135021651521446E-5	9.073842018966145E-5
16	6.992195908732735E-4	2.6687770644018073E-5	0.0	2.135021651521446E-5	1.0675108257607229E-4
17	7.632702404189169E-4	2.6687770644018073E-5	0.0	2.135021651521446E-5	1.120886367048759E-4
18	7.952955651917386E-4	2.6687770644018073E-5	0.0	2.6687770644018073E-5	1.2810129909128674E-4
19	8.273208899645603E-4	2.6687770644018073E-5	0.0	2.6687770644018073E-5	1.3877640734889397E-4
20	9.287344184118289E-4	2.6687770644018073E-5	0.0	2.6687770644018073E-5	1.3877640734889397E-4
21	0.0013183758698144927	3.2025324772821686E-5	0.0	2.6687770644018073E-5	1.3877640734889397E-4
22	0.0014197893982617615	3.2025324772821686E-5	0.0	2.6687770644018073E-5	1.4411396147769758E-4
23	0.0014624898312921904	3.2025324772821686E-5	0.0	2.6687770644018073E-5	1.494515156065012E-4
24	0.001537215589095441	3.73628789016253E-5	0.0	2.6687770644018073E-5	1.494515156065012E-4
25	0.0016813295505731386	4.270043303042892E-5	0.0	2.6687770644018073E-5	1.6012662386410842E-4
26	0.0018094308496644253	4.270043303042892E-5	0.0	3.73628789016253E-5	1.6012662386410842E-4
27	0.0018841566074676759	4.270043303042892E-5	0.0	3.73628789016253E-5	1.761392862505193E-4
28	0.0020015827983013556	4.270043303042892E-5	0.0	3.73628789016253E-5	1.8681439450812652E-4
29	0.0021510343139078568	4.270043303042892E-5	0.0	4.270043303042892E-5	1.9748950276573374E-4
30	0.0022898107212567507	4.270043303042892E-5	0.0	4.270043303042892E-5	1.9748950276573374E-4
31	0.0024979753322800914	4.270043303042892E-5	0.0	4.270043303042892E-5	2.241772734097518E-4
32	0.0027061399433034325	4.270043303042892E-5	0.0	4.270043303042892E-5	2.508650440537699E-4
33	0.0029143045543267737	4.803798715923253E-5	0.0	4.270043303042892E-5	2.7755281469778794E-4
34	0.0030690936240620782	5.3375541288036145E-5	0.0	4.270043303042892E-5	2.7755281469778794E-4
35	0.0032452329103125977	5.3375541288036145E-5	0.0	4.270043303042892E-5	2.8822792295539517E-4
36	0.003458735075464742	5.871309541683976E-5	0.0	4.270043303042892E-5	2.935654770841988E-4
37	0.003682912348874494	5.871309541683976E-5	0.0	4.270043303042892E-5	2.935654770841988E-4
38	0.003949790055314675	5.871309541683976E-5	0.0	4.270043303042892E-5	2.935654770841988E-4
39	0.004275380857171695	5.871309541683976E-5	0.0	4.270043303042892E-5	3.095781394706096E-4
40	0.004595634104899912	6.938820367444698E-5	0.0	4.270043303042892E-5	3.2025324772821684E-4
41	0.005011963326946594	6.938820367444698E-5	0.0	4.270043303042892E-5	3.2025324772821684E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	73115	0.0	41.87349	1
AGCAGTG	83440	0.0	36.877876	2
CAACGCA	84620	0.0	36.016483	13
TCAACGC	84435	0.0	35.94232	12
AGTGGTA	85540	0.0	35.909336	5
GTGGTAT	85535	0.0	35.710876	6
AACGCAG	85570	0.0	35.64409	14
GTATCAA	86020	0.0	35.602417	9
GGTATCA	86165	0.0	35.53978	8
ACGCAGA	85805	0.0	35.527298	15
ATCAACG	86145	0.0	35.370712	11
TATCAAC	86870	0.0	35.267582	10
TGGTATC	86765	0.0	35.218174	7
AGAGTAC	86935	0.0	34.857365	19
CGCAGAG	87530	0.0	34.837883	16
GCAGTGG	88270	0.0	34.817375	3
CAGTGGT	89365	0.0	34.396015	4
CAGAGTA	88700	0.0	34.198193	18
GCAGAGT	90620	0.0	33.4451	17
GTACATG	68835	0.0	33.13249	22
>>END_MODULE