FastQCFastQC Report
Thu 26 May 2016
SRR805202_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805202_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19849068
Sequences flagged as poor quality0
Sequence length53
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1211080.6101445166090418No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT606750.040.698481
TCAACGC685750.035.96879612
CAACGCA685800.035.81882513
ACGCAGA690850.035.53318815
AACGCAG694050.035.44384814
GGTATCA698600.035.2970968
GTGGTAT699300.035.295376
ATCAACG701050.035.21731611
GTATCAA701900.035.12119
AGTGGTA703600.035.0930255
TGGTATC707800.034.8549047
TATCAAC709600.034.7664910
AGCAGTG711700.034.6804162
CGCAGAG709550.034.60996616
AGAGTAC696700.034.28362719
GCAGTGG725300.034.0787283
CAGTGGT732650.033.730434
CAGAGTA725600.033.1513218
GCAGAGT747600.032.75414717
GAGTACT264950.032.5070820
Produced by FastQC (version 0.11.5)