##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805202_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	19849068
Sequences flagged as poor quality	0
Sequence length	53
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.88184246232619	37.0	35.0	37.0	35.0	37.0
2	35.95332586900302	37.0	35.0	37.0	35.0	37.0
3	37.800861884296026	39.0	38.0	39.0	35.0	39.0
4	37.883558311150935	39.0	38.0	39.0	35.0	39.0
5	37.88370008103151	39.0	38.0	39.0	35.0	39.0
6	37.86314249112351	39.0	38.0	39.0	35.0	39.0
7	37.872913881901155	39.0	38.0	39.0	35.0	39.0
8	38.84266052189453	40.0	39.0	40.0	36.0	40.0
9	38.81618421580298	40.0	39.0	40.0	36.0	40.0
10	38.84122246948824	40.0	39.0	40.0	36.0	40.0
11	38.822918436271166	40.0	39.0	40.0	36.0	40.0
12	38.80729926463046	40.0	39.0	40.0	36.0	40.0
13	38.8875082195295	40.0	40.0	40.0	36.0	40.0
14	38.82538253181459	40.0	39.0	40.0	36.0	40.0
15	38.83090581381453	40.0	39.0	40.0	36.0	40.0
16	38.86435000373821	40.0	39.0	40.0	36.0	40.0
17	38.82319764333519	40.0	39.0	40.0	36.0	40.0
18	38.7622467211055	40.0	39.0	40.0	36.0	40.0
19	38.685687912399715	40.0	39.0	40.0	36.0	40.0
20	38.66218832037857	40.0	39.0	40.0	36.0	40.0
21	38.58793203791735	40.0	39.0	40.0	36.0	40.0
22	38.607637547516084	40.0	39.0	40.0	36.0	40.0
23	38.561241716739545	40.0	39.0	40.0	36.0	40.0
24	38.21348251716403	40.0	39.0	40.0	35.0	40.0
25	38.30081372082558	40.0	39.0	40.0	35.0	40.0
26	38.38226686512435	40.0	39.0	40.0	35.0	40.0
27	38.238800884756905	40.0	39.0	40.0	35.0	40.0
28	38.358299442573326	40.0	39.0	40.0	35.0	40.0
29	38.40106794938684	40.0	39.0	40.0	35.0	40.0
30	38.39071849620345	40.0	39.0	40.0	35.0	40.0
31	38.266177333867766	40.0	39.0	40.0	35.0	40.0
32	38.061010622765764	40.0	38.0	40.0	34.0	40.0
33	38.18918858054192	40.0	38.0	40.0	35.0	40.0
34	38.14021953071046	40.0	38.0	40.0	35.0	40.0
35	38.126045212802936	40.0	38.0	40.0	35.0	40.0
36	38.09296678312553	40.0	38.0	40.0	35.0	40.0
37	38.08854390543677	40.0	38.0	40.0	35.0	40.0
38	38.064462069453334	40.0	38.0	40.0	34.0	40.0
39	38.0068929684759	40.0	38.0	40.0	34.0	40.0
40	37.94499993652095	40.0	38.0	40.0	34.0	40.0
41	37.89800503479559	40.0	38.0	40.0	34.0	40.0
42	37.81297459407162	40.0	38.0	40.0	34.0	40.0
43	37.60858645856823	40.0	38.0	40.0	33.0	40.0
44	37.547816149352705	40.0	37.0	40.0	33.0	40.0
45	37.67571978694415	40.0	38.0	40.0	33.0	40.0
46	37.7275327486409	40.0	38.0	40.0	33.0	40.0
47	37.70697541063389	40.0	37.0	40.0	33.0	40.0
48	37.67094404634011	40.0	37.0	40.0	33.0	40.0
49	37.52907602513125	40.0	37.0	40.0	33.0	40.0
50	37.51024239526007	40.0	37.0	40.0	33.0	40.0
51	37.17817179123977	40.0	37.0	40.0	33.0	40.0
52	37.13001038638187	40.0	36.0	40.0	32.0	40.0
53	36.9076189874507	39.0	36.0	40.0	32.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	8.0
11	37.0
12	93.0
13	214.0
14	456.0
15	999.0
16	1834.0
17	3119.0
18	5164.0
19	8368.0
20	12696.0
21	18814.0
22	26317.0
23	35120.0
24	45388.0
25	58431.0
26	72730.0
27	90942.0
28	112172.0
29	137930.0
30	172214.0
31	211465.0
32	262501.0
33	332313.0
34	432469.0
35	597376.0
36	888411.0
37	1510883.0
38	3292058.0
39	1.1518543E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	15.027682911862664	29.790900005985165	20.130632833743125	35.050784248409045
2	23.98193204839643	26.40891753708537	27.778160667291786	21.830989747226422
3	18.74671898952636	23.88106585155535	31.310351700140277	26.061863458778014
4	17.662829307653137	27.054756424835663	30.43657263907807	24.84584162843313
5	22.75125965612088	28.625696682584795	28.678122317884146	19.94492134341018
6	23.66728755224175	25.406406990998263	29.530978482213875	21.39532697454611
7	23.175425667341155	26.471404098167227	29.658994568409963	20.69417566608165
8	23.38097184210362	25.104448229005012	28.741767623547865	22.772812305343507
9	23.27864461948541	24.500450096699755	29.97907005003963	22.24183523377521
10	21.888226691550454	25.798062659667448	29.62074592116869	22.692964727613408
11	21.81845515366263	28.58828938467035	27.11278433828732	22.480471123379697
12	21.866386875192326	26.0070397259962	29.264119605011178	22.862453793800295
13	22.167418641520094	26.435981780101713	26.174004744202602	25.222594834175588
14	22.561165088456548	29.001396942163733	26.06640775274688	22.371030216632843
15	22.166909801508062	28.60535315814324	26.235841400714634	22.991895639634063
16	22.670792401940485	26.05580272081289	25.988011124754067	25.285393752492556
17	25.403726764400226	25.830180036664697	25.98261036739861	22.78348283153647
18	22.353104941753436	26.16150037875834	26.050598446234353	25.434796233253877
19	23.100243296058032	28.404346239329726	25.85024143199066	22.64516903262158
20	25.30207967447137	26.05847790939101	26.034597694964823	22.604844721172803
21	22.89292877630325	28.419535869391954	26.107759820259567	22.579775534045226
22	25.123441564107697	25.69566994279026	26.228868781143778	22.952019711958265
23	22.57661165753475	26.155424526733444	28.750533778210645	22.51743003752116
24	22.524009691538165	28.378591881492877	26.01124647263035	23.086151954338614
25	22.715716425577263	25.87126005110164	25.967047923862218	25.445975599458876
26	22.715922984394027	27.3798296222271	27.391825147659326	22.512422245719545
27	23.083431423581196	25.54626746202895	28.74599956028162	22.624301554108232
28	24.538084105510645	25.62442730308547	26.937995275143393	22.899493316260493
29	24.611593854179954	25.759950038964046	26.98329715027426	22.64515895658174
30	24.34954124798202	25.638604291143547	27.094647466571224	22.917206994303207
31	23.930393104603198	25.917902039531526	26.936957443039645	23.214747412825627
32	23.53541738080599	26.405093679965226	27.059592923959958	22.999896015268828
33	23.20773952711533	26.13267786678951	27.31864790830481	23.340934697790345
34	23.302343465194436	26.087305459379756	27.151098479787567	23.459252595638244
35	23.324415030468938	26.220551010253985	27.231439783469934	23.223594175807147
36	23.196585351009933	26.08267048105231	27.343344281958228	23.377399885979532
37	23.37100160067969	26.11454099507342	27.077004320807408	23.437453083439486
38	23.49288641663175	26.252643197151627	27.196924308990226	23.057546077226394
39	23.321558473173653	26.11139223262271	27.281215420290767	23.28583387391287
40	23.32150305495452	26.1617069375751	27.071956224846428	23.44483378262395
41	23.388176210590846	26.29857482477263	27.186022033880885	23.12722693075564
42	23.248345967679693	26.132259711136058	27.328859974684956	23.290534346499292
43	23.55633523951855	26.080176661191345	27.03926955159809	23.324218547692013
44	23.387561572160468	26.182574416088457	27.185946463582066	23.243917548169012
45	23.290982730272273	25.964387849343858	27.310128616618172	23.434500803765697
46	23.30382968107117	26.07292191250491	27.05830822888007	23.564940177543853
47	23.420359081847067	26.155459792872893	27.11875439189387	23.305426733386174
48	23.25958579012375	25.987099243148343	27.27502873182761	23.4782862349003
49	23.500287267895903	26.1367737769854	26.923581500149023	23.439357454969674
50	23.467383959790958	26.227856139139632	27.066535315411283	23.238224585658127
51	23.629648505410934	26.031393514294976	27.0247802063049	23.314177773989186
52	23.469439471918783	26.110031967244	26.823203991240298	23.59732456959692
53	23.49219620790256	26.248688351513533	26.832524328094397	23.426591112489515
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	5073.0
1	3550.5
2	2028.0
3	1642.5
4	1257.0
5	1308.0
6	1359.0
7	1633.0
8	2382.0
9	2857.0
10	4179.0
11	5501.0
12	8375.5
13	11250.0
14	14378.0
15	17506.0
16	24802.0
17	32098.0
18	44810.0
19	57522.0
20	77992.5
21	98463.0
22	199598.5
23	300734.0
24	266561.5
25	260648.5
26	288908.0
27	325762.0
28	362616.0
29	400189.0
30	437762.0
31	473201.0
32	508640.0
33	557818.0
34	606996.0
35	669978.5
36	732961.0
37	798848.0
38	864735.0
39	923190.0
40	981645.0
41	1048188.5
42	1172744.0
43	1230756.0
44	1279514.5
45	1328273.0
46	1360532.5
47	1392792.0
48	1406590.5
49	1420389.0
50	1407672.5
51	1394956.0
52	1358356.5
53	1321757.0
54	1267042.5
55	1212328.0
56	1131410.0
57	1050492.0
58	951054.5
59	752751.0
60	653885.0
61	572668.5
62	491452.0
63	418195.0
64	344938.0
65	281853.5
66	218769.0
67	173719.5
68	128670.0
69	99545.0
70	70420.0
71	52535.5
72	34651.0
73	25641.0
74	16631.0
75	12809.5
76	6648.5
77	4309.0
78	3117.0
79	1925.0
80	1536.5
81	1148.0
82	851.5
83	555.0
84	373.0
85	191.0
86	145.5
87	100.0
88	80.5
89	61.0
90	49.0
91	37.0
92	28.0
93	15.5
94	12.0
95	9.5
96	7.0
97	4.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	1.9849068E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.81976876174729
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.06323969089354	32.33524912264764
2	15.936402119602477	14.922773242680151
3	5.7919699078724305	8.13536075284758
4	2.7674957272338	5.182940399928407
5	1.5790551696131372	3.696549895166437
6	0.9841653965329613	2.764703777339195
7	0.6680930472649101	2.189597338899194
8	0.4799334285341577	1.7976297716001473
9	0.38435498487528486	1.6195870362857145
>10	2.1735729186561987	18.911299588173623
>50	0.13156900419924272	4.150647493589625
>100	0.03890491206856265	2.9938542654939595
>500	9.731978505790326E-4	0.31067430874966184
>1k	2.16461229936831E-4	0.2088141715250548
>5k	3.242014353364478E-5	0.09507136361653871
>10k+	2.1613429022427757E-5	0.6852474714570297
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	121108	0.6101445166090418	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0012998091396533077	0.0	0.0	0.0	5.038019921136851E-6
2	0.0013753794384703604	0.0	0.0	5.038019921136851E-6	5.038019921136851E-6
3	0.0014005695380760446	0.0	0.0	1.5114059763410554E-5	1.5114059763410554E-5
4	0.001425759637681729	0.0	0.0	2.0152079684547405E-5	1.5114059763410554E-5
5	0.003178990570237353	0.0	0.0	2.5190099605684257E-5	1.5114059763410554E-5
6	0.0031991426499219005	0.0	0.0	2.5190099605684257E-5	2.0152079684547405E-5
7	0.0031991426499219005	0.0	0.0	4.030415936909481E-5	2.0152079684547405E-5
8	0.0032041806698430374	0.0	0.0	4.534217929023166E-5	2.5190099605684257E-5
9	0.0032092186897641744	0.0	0.0	4.534217929023166E-5	3.526613944795796E-5
10	0.003219294729606448	0.0	0.0	4.534217929023166E-5	4.030415936909481E-5
11	0.0034107394866096483	0.0	0.0	4.534217929023166E-5	5.038019921136851E-5
12	0.0034711957256632906	0.0	0.0	4.534217929023166E-5	7.053227889591592E-5
13	0.0036777545424299014	1.0076039842273703E-5	0.0	4.534217929023166E-5	7.557029881705278E-5
14	0.0037230967217201333	1.0076039842273703E-5	0.0	4.534217929023166E-5	8.060831873818962E-5
15	0.0037734769209315016	1.0076039842273703E-5	0.0	4.534217929023166E-5	8.060831873818962E-5
16	0.003793629000616049	1.0076039842273703E-5	0.0	4.534217929023166E-5	9.068435858046332E-5
17	0.00382385712014287	1.0076039842273703E-5	0.0	4.534217929023166E-5	9.068435858046332E-5
18	0.003838971179906281	1.0076039842273703E-5	0.0	6.549425897477907E-5	9.068435858046332E-5
19	0.0038591232595908283	1.0076039842273703E-5	0.0	7.053227889591592E-5	1.0076039842273703E-4
20	0.003874237319354239	1.0076039842273703E-5	0.0	7.557029881705278E-5	1.0076039842273703E-4
21	0.0039044654388810597	1.0076039842273703E-5	0.0	7.557029881705278E-5	1.1083643826501073E-4
22	0.003919579498644471	1.0076039842273703E-5	0.0	7.557029881705278E-5	1.1587445818614759E-4
23	0.0039498076181712916	1.0076039842273703E-5	0.0	8.060831873818962E-5	1.1587445818614759E-4
24	0.003985073757619249	1.0076039842273703E-5	0.0	8.060831873818962E-5	1.259504980284213E-4
25	0.004020339897067207	1.0076039842273703E-5	0.0	9.068435858046332E-5	1.259504980284213E-4
26	0.004050568016594028	1.0076039842273703E-5	0.0	9.572237850160018E-5	1.3098851794955815E-4
27	0.0040959101958842606	1.0076039842273703E-5	0.0	9.572237850160018E-5	1.3098851794955815E-4
28	0.004166442474780176	1.0076039842273703E-5	0.0	1.0076039842273703E-4	1.3098851794955815E-4
29	0.004236974753676092	1.0076039842273703E-5	0.0	1.0076039842273703E-4	1.3098851794955815E-4
30	0.004327659112256555	1.0076039842273703E-5	0.0	1.1083643826501073E-4	1.461025777129687E-4
31	0.004403229411073608	1.0076039842273703E-5	0.0	1.1083643826501073E-4	1.461025777129687E-4
32	0.004433457530600429	1.0076039842273703E-5	0.0	1.1587445818614759E-4	1.6121663747637924E-4
33	0.004574522088392261	1.0076039842273703E-5	0.0	1.1587445818614759E-4	1.763306972397898E-4
34	0.004700472586420683	1.0076039842273703E-5	0.0	1.1587445818614759E-4	1.8136871716092665E-4
35	0.004826423084449104	1.0076039842273703E-5	0.0	1.259504980284213E-4	1.964827769243372E-4
36	0.004962449622319799	1.0076039842273703E-5	0.0	1.259504980284213E-4	2.166348566088846E-4
37	0.005199236558613231	1.0076039842273703E-5	0.0	1.3098851794955815E-4	2.166348566088846E-4
38	0.00536045319608961	1.0076039842273703E-5	0.0	1.3602653787069498E-4	2.166348566088846E-4
39	0.005491441714039168	1.0076039842273703E-5	0.0	1.3602653787069498E-4	2.166348566088846E-4
40	0.0057181526104903265	1.0076039842273703E-5	0.0	1.4106455779183184E-4	2.166348566088846E-4
41	0.005944863506941484	1.0076039842273703E-5	0.0	1.4106455779183184E-4	2.166348566088846E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	60675	0.0	40.69848	1
TCAACGC	68575	0.0	35.968796	12
CAACGCA	68580	0.0	35.818825	13
ACGCAGA	69085	0.0	35.533188	15
AACGCAG	69405	0.0	35.443848	14
GGTATCA	69860	0.0	35.297096	8
GTGGTAT	69930	0.0	35.29537	6
ATCAACG	70105	0.0	35.217316	11
GTATCAA	70190	0.0	35.1211	9
AGTGGTA	70360	0.0	35.093025	5
TGGTATC	70780	0.0	34.854904	7
TATCAAC	70960	0.0	34.76649	10
AGCAGTG	71170	0.0	34.680416	2
CGCAGAG	70955	0.0	34.609966	16
AGAGTAC	69670	0.0	34.283627	19
GCAGTGG	72530	0.0	34.078728	3
CAGTGGT	73265	0.0	33.73043	4
CAGAGTA	72560	0.0	33.15132	18
GCAGAGT	74760	0.0	32.754147	17
GAGTACT	26495	0.0	32.50708	20
>>END_MODULE